Attenuating effect of daidzein on polychlorinated biphenyls-induced oxidative toxicity in mouse testicular cells

The attenuating effect of daidzein (DAI) on oxidative toxicity induced by Aroelor 1254 (A1254) was investigated in mouse testicular cells. Cells were exposed to A1254 alone or with DAI. The oxidative damage was estimated by measuring malondialdehyde (MDA) formation, superoxide dismutase (SOD) activity and glutathione (GSH) content. Results show that A1254 induced a decrease of germ cell number, an elevation in thiobarbituric acid reactive substances (TBARS) but a decrease in SOD activity and GSH content. However, simultaneous supplementation with DAI decreased TBARS level and increased SOD activity and GSH content. Consequently, dietary DAI may restore the intracellular antioxidant system to attenuate the oxidative toxicity of A1254 in testicular cells.

[1]  Jianying Zhang,et al.  Occurrence and congeners specific of polychlorinated biphenyls in agricultural soils from Southern Jiangsu, China. , 2007, Journal of environmental sciences.

[2]  J. Arunakaran,et al.  Ameliorative effect of vitamins (alpha-tocopherol and ascorbic acid) on PCB (Aroclor 1254) induced oxidative stress in rat epididymal sperm. , 2007, Reproductive toxicology.

[3]  Yuling Zhang.  Protective effect of quercetin on aroclor 1254-induced oxidative damage in cultured chicken spermatogonial cells. , 2005, Toxicological sciences : an official journal of the Society of Toxicology.

[4]  Caiqiao Zhang,et al.  Protective effects of antioxidant vitamins on Aroclor 1254-induced toxicity in cultured chicken embryo hepatocytes. , 2005, Toxicology in vitro : an international journal published in association with BIBRA.

[5]  Y. Mi,et al.  Toxic and hormonal effects of polychlorinated biphenyls on cultured testicular germ cells of embryonic chickens. , 2005, Toxicology letters.

[6]  K. Balasubramanian,et al.  Impact of polychlorinated biphenyl Aroclor 1254 on testicular antioxidant system in adult rats , 2005, Human & experimental toxicology.

[7]  K. Balasubramanian,et al.  Effects of Vitamin C and E on PCB (Aroclor 1254) induced oxidative stress, androgen binding protein and lactate in rat Sertoli cells. , 2004, Reproductive toxicology.

[8]  R Hoogerbrugge,et al.  Dioxins, dioxin-like PCBs and non-dioxin-like PCBs in foodstuffs: occurrence and dietary intake in The Netherlands. , 2004, Toxicology letters.

[9]  Zhang Cai-qiao,et al.  Effect of polychlorinated biphenyls on spermatogenesis and testosterone secretion in adult cocks , 2004, Journal of Zhejiang University. Science.

[10]  Caiqiao Zhang,et al.  Effect of polychlorinated biphenyls on spermatogenesis and testosterone secretion in adult cocks. , 2004, Journal of Zhejiang University. Science.

[11]  S. Stojilkovic,et al.  Inhibition of Rat Testicular Androgenesis by a Polychlorinated Biphenyl Mixture Aroclor 12481 , 2000, Biology of reproduction.

[12]  P. Renard,et al.  Reactive oxygen species, lipid peroxidation and enzymatic defence systems in human spermatozoa. , 1995, Journal of reproduction and fertility.

[13]  H. Issaq,et al.  Promotion by polychlorinated biphenyls of lung and liver tumors in mice. , 1994, Carcinogenesis.