Low cost lab-on-a-chip prototyping with a consumer grade 3D printer.

Versatile prototyping of 3D printed lab-on-a-chip devices, supporting different forms of sample delivery, transport, functionalization and readout, is demonstrated with a consumer grade printer, which centralizes all critical fabrication tasks. Devices cost 0.57US$ and are demonstrated in chemical sensing and micromixing examples, which exploit established principles from reference technologies.

[1]  Luke P. Lee,et al.  Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS). , 2011, Lab on a chip.

[2]  M. Tabrizian,et al.  Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics. , 2012, Lab on a chip.

[3]  Philip J. Kitson,et al.  Configurable 3D-Printed millifluidic and microfluidic 'lab on a chip' reactionware devices. , 2012, Lab on a chip.

[4]  J. González-López,et al.  Xeroprotectants for the stabilization of biomaterials. , 2012, Biotechnology advances.

[5]  Nicholas X. Fang,et al.  Projection micro-stereolithography using digital micro-mirror dynamic mask , 2005 .

[6]  Aliaa I. Shallan,et al.  Cost-effective three-dimensional printing of visibly transparent microchips within minutes. , 2014, Analytical chemistry.

[7]  R H Gadsden,et al.  Semi-automated vs. visual reading of urinalysis dipsticks. , 1977, Clinical chemistry.

[8]  J. Lewis,et al.  Chaotic mixing in three-dimensional microvascular networks fabricated by direct-write assembly , 2003, Nature materials.

[9]  Bastian E. Rapp,et al.  Let there be chip—towards rapid prototyping of microfluidic devices: one-step manufacturing processes , 2011 .

[10]  Qinjian Zhao,et al.  Complexities in horseradish peroxidase-catalyzed oxidation of dihydroxyphenoxazine derivatives: appropriate ranges for pH values and hydrogen peroxide concentrations in quantitative analysis. , 2004, Analytical biochemistry.

[11]  R. Haugland,et al.  A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. , 1997, Analytical biochemistry.

[12]  George M Whitesides,et al.  Prototyping of microfluidic devices in poly(dimethylsiloxane) using solid-object printing. , 2002, Analytical chemistry.

[13]  George M. Whitesides,et al.  Microscope Projection Photolithography for Rapid Prototyping of Masters with Micron-Scale Features for Use in Soft Lithography , 2001 .

[14]  A. Turner,et al.  Home blood glucose biosensors: a commercial perspective. , 2005, Biosensors & bioelectronics.

[15]  I. Benzie,et al.  Hydrogen peroxide in urine as a potential biomarker of whole body oxidative stress , 2003, Free radical research.

[16]  Samuel K Sia,et al.  Commercialization of microfluidic point-of-care diagnostic devices. , 2012, Lab on a chip.

[17]  Daniel Filippini,et al.  Fabrication of monolithic 3D micro-systems. , 2011, Lab on a chip.

[18]  Pawan K. Singal,et al.  Oxidative stress and heart failure , 1995, Molecular and Cellular Biochemistry.

[19]  F. He,et al.  Direct laser writing of sub-50 nm nanofluidic channels buried in glass for three-dimensional micro-nanofluidic integration. , 2013, Lab on a chip.

[20]  Brian Evans,et al.  Practical 3D Printers , 2012, Apress.

[21]  Barry Halliwell,et al.  Oxidative stress and cancer: have we moved forward? , 2007, The Biochemical journal.

[22]  Keith R. Lykke,et al.  Projection photolithography utilizing a Schwarzschild microscope and self-assembled alkanethiol monolayers as simple photoresists , 1996 .

[23]  S. Clarke,et al.  A history of blood glucose meters and their role in self-monitoring of diabetes mellitus , 2012, British journal of biomedical science.

[24]  Xuefei Sun,et al.  Surface-reactive acrylic copolymer for fabrication of microfluidic devices. , 2005, Analytical chemistry.

[25]  Daniel Filippini,et al.  PDMS lab-on-a-chip fabrication using 3D printed templates. , 2014, Lab on a chip.

[26]  D. K. Chattopadhyay,et al.  THERMAL AND MECHANICAL PROPERTIES OF EPOXY ACRYLATE/METHACRYLATES UV CURED COATINGS , 2005 .

[27]  E. Delamarche,et al.  Capillary pumps for autonomous capillary systems. , 2007, Lab on a chip.

[28]  Sangmo Kang,et al.  A Review on Mixing in Microfluidics , 2010, Micromachines.

[29]  Nam-Trung Nguyen,et al.  Micromixers?a review , 2005 .

[30]  João Paulo Davim,et al.  State of the Art on Micromilling of Materials, a Review , 2012 .

[31]  Nae Yoon Lee,et al.  Microfluidic immunoassay platform using antibody-immobilized glass beads and its application for detection of Escherichia coli O157:H7 , 2006 .

[32]  Philip J. Kitson,et al.  Integrated 3D-printed reactionware for chemical synthesis and analysis. , 2012, Nature chemistry.

[33]  D. Bodas,et al.  Hydrophilization and hydrophobic recovery of PDMS by oxygen plasma and chemical treatment—An SEM investigation , 2007 .