Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles

Degradation products of titanium implants include free ions, organo-metallic complexes, and particles, ranging from nano to macro sizes. The biological effects, especially of nanoparticles, is yet unknown. The main objective of this study was to develop Ti-protein antigens in physiological solutions that can be used in testing of cellular responses. For this purpose, 0.1% TiO2 nanoparticles less than 100 nm were mixed with human serum albumin (HSA), 0.1% and 1%, in cell culture medium (DMEM, pH 7.2). The Ti concentrations in the resulting solutions were analyzed by inductively coupled plasma mass spectrometry. The stability of the nanoparticles in suspension was analyzed by UV-vis spectrophotometer and Dynamic Light Scattering. The concentration of Ti in suspension was dependent on the presence and concentration of HSA. Albumin prevented high aggregation rate of TiO2 nanoparticles in cell culture medium. It is shown that nano TiO2-protein stable aggregates can be produced under physiological conditions at high concentrations, and are candidates for use in cellular tests.

[1]  Lothar Borchers,et al.  Nonalloyed titanium as a bioinert metal--a review. , 2005, Quintessence international.

[2]  Charles DiMarzio,et al.  Surface functionalization of gold nanoparticles using hetero-bifunctional poly(ethylene glycol) spacer for intracellular tracking and delivery , 2006, International journal of nanomedicine.

[3]  J. Verbov.  Pacemaker contact sensitivity , 1985, Contact dermatitis.

[4]  Robert N Grass,et al.  Oxide nanoparticle uptake in human lung fibroblasts: effects of particle size, agglomeration, and diffusion at low concentrations. , 2005, Environmental science & technology.

[5]  John R. Adams,et al.  Granulomatous reaction to titanium alloy: an unusual reaction to ear piercing. , 2006, Journal of the American Academy of Dermatology.

[6]  T. Wright,et al.  Metal levels in cemented total hip arthroplasty. A comparison of well-fixed and loose implants. , 1992, Clinical orthopaedics and related research.

[7]  W. B. Stern,et al.  Allergic contact dermatitis from “titanium” spectacle frames , 2001, Contact dermatitis.

[8]  N. Baldini,et al.  Sensitivity to implant materials in patients undergoing total hip replacement. , 2006, Journal of biomedical materials research. Part B, Applied biomaterials.

[9]  Navid B. Saleh,et al.  Aggregation and sedimentation of aqueous nanoscale zerovalent iron dispersions. , 2007, Environmental science & technology.

[10]  A CAUSE OF IMPLANT FAILURE , 1991 .

[11]  P. Wooley,et al.  The Immune Response to Implant Materials in Humans , 1996, Clinical orthopaedics and related research.

[12]  R. Mann,et al.  Human Physiology , 1839, Nature.

[13]  T. Tsuji,et al.  Pacemaker dermatitis from titanium. , 2000, Contact dermatitis.

[14]  J. Jacobs,et al.  Metal sensitivity in patients with orthopaedic implants. , 2001, The Journal of bone and joint surgery. American volume.

[15]  J. Galante,et al.  Systemic metal-protein binding associated with total joint replacement arthroplasty. , 2000, Journal of biomedical materials research.

[16]  K. Mikecz,et al.  A triple assay technique for the evaluation of metal-induced, delayed-type hypersensitivity responses in patients with or receiving total joint arthroplasty. , 2000, Journal of biomedical materials research.

[17]  M. Hiraoka,et al.  A case of allergic reaction to surgical metal clips inserted for postoperative boost irradiation in a patient undergoing Breast-conserving therapy , 2001, Breast cancer.

[18]  K. Mikecz,et al.  Differential lymphocyte reactivity to serum-derived metal-protein complexes produced from cobalt-based and titanium-based implant alloy degradation. , 2001, Journal of biomedical materials research.

[19]  N. Gjerdet,et al.  Metal particles and tissue changes adjacent to miniplates. A retrieval study. , 1995, Acta odontologica Scandinavica.

[20]  H. Mckellop,et al.  In vivo wear of titanium-alloy hip prostheses. , 1990, The Journal of bone and joint surgery. American volume.

[21]  Jeremy J. W. Chen,et al.  Titanium dioxide nanoparticles induce emphysema‐like lung injury in mice , 2006, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[22]  R. Schmelzeisen,et al.  Ultrastructural findings in soft tissues adjacent to titanium plates used in jaw fracture treatment. , 1993, International journal of oral and maxillofacial surgery.

[23]  Buddy D. Ratner,et al.  Biomaterials Science: An Introduction to Materials in Medicine , 1996 .

[24]  J. Frame,et al.  Allergic responses to titanium. , 1998, Journal of oral and maxillofacial surgery.

[25]  N. Balato,et al.  Acne and allergic contact dermatitis , 1996, Contact dermatitis.

[26]  S. Fukuda,et al.  Lymphocytoma cutis due to pierced earrings , 2006, Journal of cutaneous pathology.

[27]  M. Freeman,et al.  Sensitivity to titanium. A cause of implant failure? , 1991, The Journal of bone and joint surgery. British volume.

[28]  M. Wimmer,et al.  Subsurface microstructure of metal-on-metal hip joints and its relationship to wear particle generation. , 2005, Journal of biomedical materials research. Part B, Applied biomaterials.

[29]  A. Fernandez-Barbero,et al.  The aggregation behaviour of protein-coated particles: a light scattering study , 2003, European Biophysics Journal.

[30]  P. Campbell,et al.  Immune responses correlate with serum-metal in metal-on-metal hip arthroplasty. , 2004, The Journal of arthroplasty.

[31]  D. Scharnweber,et al.  Electrochemical behavior of titanium-based materials – are there relations to biocompatibility? , 2002, Journal of materials science. Materials in medicine.

[32]  J. Griffin.  Human Physiology, The Mechanisms of Body Function , 1971 .

[33]  Thomas Jay Webster,et al.  Nanomedicine for implants: a review of studies and necessary experimental tools. , 2007, Biomaterials.

[34]  O. Cámara,et al.  Adsorption of human serum albumin (HSA) onto colloidal TiO2 particles, Part I. , 2003, Journal of colloid and interface science.

[35]  Navid B. Saleh,et al.  Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): implications for nanoparticle neurotoxicity. , 2006, Environmental science & technology.

[36]  N. H. Tkachenko,et al.  Effect of 1-1-charged ions on aggregative stability and electrical surface properties of aqueous suspensions of titanium dioxide , 2006 .

[37]  N. Dourov,et al.  Analysis of titanium dental implants after failure of osseointegration: combined histological, electron microscopy, and X-ray photoelectron spectroscopy approach. , 1998, Journal of biomedical materials research.

[38]  B. Przybilla,et al.  Hypersensitivity to titanium osteosynthesis with impaired fracture healing, eczema, and T‐cell hyperresponsiveness in vitro: case report and review of the literature , 2006, Contact dermatitis.

[39]  S. A. Gómez-Lopera,et al.  Colloidal stability of magnetite/poly(lactic acid) core/shell nanoparticles. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[40]  M. Sela,et al.  Mechanism of adsorption of human albumin to titanium in vitro. , 1997, Journal of biomedical materials research.

[41]  D. S. Luciano,et al.  Human Physiology: The Mechanism of Body Function , 1975 .

[42]  Tom Duerig,et al.  Self-expanding nitinol stents: material and design considerations , 2004, European Radiology.

[43]  A. C. Johannessen,et al.  Host response to titanium dental implant placement evaluated in a human oral model. , 2006, Journal of periodontology.

[44]  Claude Degueldre,et al.  Colloid analysis by single particle inductively coupled plasma-mass spectroscopy: a feasibility study , 2003 .