Fast numerical reconstruction technique for high-resolution hybrid holographic microscopy.

A numerical reconstruction method believed to be new is proposed for hybrid holographic microscopy in which the hologram of a microscopic object is recorded by an image sensor and is then reconstructed by a computer. Because the Fresnel-Kirchhoff integral must be used for numerical reconstruction to achieve high resolution, we propose an approximation technique for reducing the calculation time. This approximation technique is suitable for microscopic application. The numerical reconstruction of 1-microm-sized objects was demonstrated with a He-Ne laser (lambda = 0.6328 microm).