Moderately Thermophilic Magnetotactic Bacteria from Hot Springs in Nevada

ABSTRACT Populations of a moderately thermophilic magnetotactic bacterium were discovered in Great Boiling Springs, Nevada, ranging from 32 to 63°C. Cells were small, Gram-negative, vibrioid to helicoid in morphology, and biomineralized a chain of bullet-shaped magnetite magnetosomes. Phylogenetically, based on 16S rRNA gene sequencing, the organism belongs to the phylum Nitrospirae.

[1]  R. Thauer,et al.  A ‘capillary racetrack’ method for isolation of magnetotactic bacteria , 1987 .

[2]  E. Stackebrandt,et al.  Nucleic acid techniques in bacterial systematics , 1991 .

[3]  K. Schleifer,et al.  Dominating Role of an Unusual Magnetotactic Bacterium in the Microaerobic Zone of a Freshwater Sediment , 1993, Applied and environmental microbiology.

[4]  D. Schüler,et al.  Combined Approach for Characterization of Uncultivated Magnetotactic Bacteria from Various Aquatic Environments , 2005, Applied and Environmental Microbiology.

[5]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[6]  T. A. Hall,et al.  BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT , 1999 .

[7]  R. Kopp,et al.  The identification and biogeochemical interpretation of fossil magnetotactic bacteria , 2008 .

[8]  R. Amann,et al.  Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes , 2001 .

[9]  J. Anderson.  A Geochemical Study of the Southwest Part of the Black Rock Desert and Its Geothermal Areas ; Washoe , Pershing , and Humboldt Counties , Nevada , 2010 .

[10]  Robert F. Butler,et al.  Theoretical single‐domain grain size range in magnetite and titanomagnetite , 1975 .

[11]  R. Amann,et al.  Fluorescence in situ hybridization with rRNA-targeted oligonucleotide probes , 2001 .

[12]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[13]  D. Lane.  16S/23S rRNA sequencing , 1991 .

[14]  C. Romanek,et al.  Magnetite as a prokaryotic biomarker: A review , 2010 .

[15]  D. Schüler,et al.  The biomineralization of magnetosomes in Magnetospirillum gryphiswaldense , 2002, International microbiology : the official journal of the Spanish Society for Microbiology.

[16]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[17]  E. Shock,et al.  Microbiology and geochemistry of great boiling and mud hot springs in the United States Great Basin , 2009, Extremophiles.

[18]  F. Elbaz-Poulichet,et al.  Thermodesulfovibrio hydrogeniphilus sp. nov., a new thermophilic sulphate-reducing bacterium isolated from a Tunisian hot spring. , 2008, Systematic and applied microbiology.

[19]  R. Frankel,et al.  Magnetosome formation in prokaryotes , 2004, Nature Reviews Microbiology.