Characterization of a Novel IncHI2 Plasmid Carrying Tandem Copies of blaCTX-M-2 in a fosA6-Harboring Escherichia coli Sequence Type 410 Strain

ABSTRACT The extended-spectrum β-lactamase gene blaCTX-M-2 is mainly associated with ISCR1 embedded in complex sul1-type integrons, but information on the genetic context of plasmids harboring the ISCR1-blaCTX-M-2 module remains limited. In this study, a blaCTX-M-2-harboring plasmid (pYD786-1) belonging to the sequence type 2 (ST2)-IncHI2 plasmid type and isolated from an Escherichia coli ST410 clinical strain was sequenced and analyzed. pYD786-1 belongs to the APEC-O1-R-type IncHI2 plasmids, which are widely distributed in human, poultry, and livestock strains. It contains a multidrug resistance mosaic region (MRR) consisting of a Tn21::In2 transposon backbone augmented by acquisition of duplicate ISCR1-blaCTX-M-2 modules. Tn2411, a Tn21::In2 precursor, likely played a role in the generation of the MRR in pN13-01290_23, the putative progenitor plasmid of pYD786-1, found in a foodborne Salmonella strain. Tn21/Tn2411::In::ISCR1-blaCTX-M-2 derivatives, including pYD786-1, have been identified in strains from Europe, South America, and the United States, suggesting potential global dissemination of the blaCTX-M-2 modules mediated by this vehicle.

[1]  T. Chakraborty,et al.  Comparative genome analysis of IncHI2 VIM-1 carbapenemase-encoding plasmids of Escherichia coli and Salmonella enterica isolated from a livestock farm in Germany. , 2017, Veterinary microbiology.

[2]  V. Cooper,et al.  Glutathione-S-transferase FosA6 of Klebsiella pneumoniae origin conferring fosfomycin resistance in ESBL-producing Escherichia coli. , 2016, The Journal of antimicrobial chemotherapy.

[3]  J. Klumpp,et al.  Full-Length Nucleotide Sequences of mcr-1-Harboring Plasmids Isolated from Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates of Different Origins , 2016, Antimicrobial Agents and Chemotherapy.

[4]  Jian Sun,et al.  Co-spread of metal and antibiotic resistance within ST3-IncHI2 plasmids from E. coli isolates of food-producing animals , 2016, Scientific Reports.

[5]  John H. E. Nash,et al.  Dissemination of the mcr-1 colistin resistance gene. , 2016, The Lancet. Infectious diseases.

[6]  J. Rolain,et al.  Dissemination of the mcr-1 colistin resistance gene. , 2016, The Lancet. Infectious diseases.

[7]  Jian-Hua Liu,et al.  Dissemination of the mcr-1 colistin resistance gene. , 2016, The Lancet. Infectious diseases.

[8]  Jian Sun,et al.  Prevalence of extended-spectrum cephalosporin-resistant Escherichia coli in a farrowing farm: ST1121 clone harboring IncHI2 plasmid contributes to the dissemination of blaCMY-2 , 2015, Front. Microbiol..

[9]  A. Butt,et al.  Fosfomycin Resistance in Escherichia coli, Pennsylvania, USA , 2015, Emerging infectious diseases.

[10]  M. Hattori,et al.  Complete Nucleotide Sequence of the IncN Plasmid Encoding IMP-6 and CTX-M-2 from Emerging Carbapenem-Resistant Enterobacteriaceae in Japan , 2014, Antimicrobial Agents and Chemotherapy.

[11]  J. Boncy,et al.  Multidrug-resistant IncA/C plasmid in Vibrio cholerae from Haiti. , 2014, Emerging infectious diseases.

[12]  Ole Lund,et al.  In Silico Detection and Typing of Plasmids using PlasmidFinder and Plasmid Multilocus Sequence Typing , 2014, Antimicrobial Agents and Chemotherapy.

[13]  Jian-Hua Liu,et al.  Escherichia coli from chickens in China , 2014 .

[14]  Ana Tereza Ribeiro de Vasconcelos,et al.  Comparative analysis of the complete genome of KPC-2-producing Klebsiella pneumoniae Kp13 reveals remarkable genome plasticity and a wide repertoire of virulence and resistance mechanisms , 2014, BMC Genomics.

[15]  C. Woods,et al.  Widespread Dissemination of CTX-M-15 Genotype Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae among Patients Presenting to Community Hospitals in the Southeastern United States , 2013, Antimicrobial Agents and Chemotherapy.

[16]  P. H. Roy,et al.  "Distribution and functional identification of complex class 1 integrons". , 2013, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[17]  M. Inoue,et al.  Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan. , 2012, Journal of medical microbiology.

[18]  D. Pakstis,et al.  Clinical characteristics of bacteraemia caused by extended-spectrum β-lactamase-producing Enterobacteriaceae in the era of CTX-M-type and KPC-type β-lactamases. , 2012, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[19]  J. Quinn,et al.  Molecular Epidemiological Analysis of Escherichia coli Sequence Type ST131 (O25:H4) and blaCTX-M-15 among Extended-Spectrum-β-Lactamase-Producing E. coli from the United States, 2000 to 2009 , 2012, Antimicrobial Agents and Chemotherapy.

[20]  K. Shutt,et al.  Clinical and Microbiologic Characteristics of Cephalosporin-Resistant Escherichia coli at Three Centers in the United States , 2012, Antimicrobial Agents and Chemotherapy.

[21]  S. Partridge,et al.  Analysis of antibiotic resistance regions in Gram-negative bacteria. , 2011, FEMS microbiology reviews.

[22]  E. Clímaco,et al.  CTX-M-producing Klebsiella spp. in a Brazilian hospital: what has changed in 6 years? , 2010, Diagnostic microbiology and infectious disease.

[23]  A. Carattoli,et al.  Plasmid double locus sequence typing for IncHI2 plasmids, a subtyping scheme for the characterization of IncHI2 plasmids carrying extended-spectrum beta-lactamase and quinolone resistance genes. , 2010, The Journal of antimicrobial chemotherapy.

[24]  D. Mazel,et al.  Inverse Correlation between Promoter Strength and Excision Activity in Class 1 Integrons , 2010, PLoS genetics.

[25]  M. Labbate,et al.  Urinary Tract Infections in a South American Population: Dynamic Spread of Class 1 Integrons and Multidrug Resistance by Homologous and Site-Specific Recombination , 2008, Journal of Clinical Microbiology.

[26]  F. Weill,et al.  Comparative Analysis of IncHI2 Plasmids Carrying blaCTX-M-2 or blaCTX-M-9 from Escherichia coli and Salmonella enterica Strains Isolated from Poultry and Humans , 2007, Antimicrobial Agents and Chemotherapy.

[27]  T. Johnson,et al.  Complete DNA Sequence, Comparative Genomics, and Prevalence of an IncHI2 Plasmid Occurring among Extraintestinal Pathogenic Escherichia coli Isolates , 2006, Antimicrobial Agents and Chemotherapy.

[28]  P. Bennett,et al.  Common regions e.g. orf513 and antibiotic resistance: IS91-like elements evolving complex class 1 integrons. , 2006, The Journal of antimicrobial chemotherapy.

[29]  M. Galas,et al.  Vibrio cholerae InV117, a Class 1 Integron Harboring aac(6′)-Ib and blaCTX-M-2, Is Linked to Transposition Genes , 2006, Antimicrobial Agents and Chemotherapy.

[30]  F. Baquero,et al.  In117, an Unusual In0-Like Class 1 Integron Containing CR1 and blaCTX-M-2 and Associated with a Tn21-Like Element , 2006, Antimicrobial Agents and Chemotherapy.

[31]  M. Gilmour,et al.  The complete nucleotide sequence of the resistance plasmid R478: defining the backbone components of incompatibility group H conjugative plasmids through comparative genomics. , 2004, Plasmid.

[32]  R. Bonnet.  Growing Group of Extended-Spectrum β-Lactamases: the CTX-M Enzymes , 2004, Antimicrobial Agents and Chemotherapy.

[33]  P. H. Roy,et al.  bla CTX-M-2 Is Located in an Unusual Class 1 Integron (In35) Which Includes Orf513 , 2002, Antimicrobial Agents and Chemotherapy.

[34]  R. Hall,et al.  Transposons Tn1696 and Tn21and Their Integrons In4 and In2 Have Independent Origins , 2001, Antimicrobial Agents and Chemotherapy.

[35]  R. Hall,et al.  Transposon Tn21, Flagship of the Floating Genome , 1999, Microbiology and Molecular Biology Reviews.

[36]  R. Hall,et al.  The integrons In0, In2, and In5 are defective transposon derivatives , 1996, Journal of bacteriology.

[37]  J. Mullins,et al.  Photonic detection of bacterial pathogens in living hosts , 1995, Molecular microbiology.

[38]  R M Hall,et al.  Mobile gene cassettes and integrons: capture and spread of genes by site‐specific recombination , 1995, Molecular microbiology.