A plug and play microfluidic device.

Chip-to-world interface is a major issue in the field of microfluidics and its applications. We developed a plug and play microfluidic device composed of a fluid driving unit and a polymer chip containing microfluidic channels and reservoirs. The one and only connection of the device to the external world is a set of electric control lines for the driving unit. Just putting the reagents and samples onto the reservoirs, the chip can be operated for chemical or biochemical reaction and analysis. We demonstrate here that silicon-based micropumps embedded in the present device allow us to achieve flexible fluidic manipulations with minimum time delay and dead volume.

[1]  D. J. Harrison,et al.  Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip , 1993, Science.

[2]  Nickolaj J. Petersen,et al.  Monolithic integration of optical waveguides for absorbance detection in microfabricated electrophoresis devices , 2001, Electrophoresis.

[3]  S. Jacobson,et al.  High-Speed Separations on a Microchip , 1994 .

[4]  Alan P. Morrison,et al.  Development of a microfluidic device for fluorescence activated cell sorting , 2002 .

[5]  S. Quake,et al.  Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.

[6]  G. Whitesides,et al.  Microfabrication inside capillaries using multiphase laminar flow patterning , 1999, Science.

[7]  G. Whitesides,et al.  Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device , 2002, Nature Biotechnology.

[8]  N F de Rooij,et al.  Electrokinetically driven microfluidic chips with surface-modified chambers for heterogeneous immunoassays. , 2001, Analytical chemistry.

[9]  Igor L. Medintz,et al.  Microfabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis. , 2002, Analytical chemistry.

[10]  A Manz,et al.  Chemical amplification: continuous-flow PCR on a chip. , 1998, Science.

[11]  T Fujii,et al.  Microfabricated Polymer Chip for Capillary Gel Electrophoresis , 2001, Biotechnology progress.

[12]  S. Masuda,et al.  Novel method of cell fusion in field constriction area in fluid integration circuit , 1989 .

[13]  Brian N. Johnson,et al.  An integrated nanoliter DNA analysis device. , 1998, Science.

[14]  Takehiko Kitamori,et al.  Continuous-flow chemical processing on a microchip by combining microunit operations and a multiphase flow network. , 2002, Analytical chemistry.

[15]  T. Fujii,et al.  Handling of Picoliter Liquid Samples in a Poly(dimethylsiloxane)-Based Microfluidic Device , 1999 .

[16]  S. Quake,et al.  A microfabricated fluorescence-activated cell sorter , 1999, Nature Biotechnology.

[17]  M. A. Northrup,et al.  Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device. , 1996, Analytical chemistry.

[18]  L. Idahl,et al.  Regulatory effects of ATP and luciferin on firefly luciferase activity. , 1995, The Biochemical journal.

[19]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[20]  George M. Whitesides,et al.  Laminar flows: Subcellular positioning of small molecules , 2001, Nature.

[21]  Kenneth S. Johnson,et al.  In situ osmotic analyzer for the year-long continuous determination of Fe in hydrothermal systems , 2002 .