Shear Stress-Induced Changes in Platelet Reactivity

Summary We have investigated the effects on platelet function of a physiologic shear stress. The aggregation to thrombin and collagen, the release reaction [(14C) serotonin] and the procoagulant activity of washed platelet suspensions were assayed on samples undergoing laminar oscillatory flow for 20 minutes at 37° C in polyethylene tubes, and on paired samples kept at rest. The pulse rate was established at 72 cycles per minute and the shear stress at the wall estimated at 26.2 dynes/cm2. The platelet suspensions were prepared at 37°C from blood samples of 15 healthy volunteers and 15 patients with the diagnosis of coronary artery disease proven by angiography. Our results show enhancement of platelet aggregation in samples undergoing oscillatory flow. Furthermore, platelets from coronary patients gained additional procoagulant activity. However, no change was encountered in the rate and speed of the release reaction. It is concluded that exposure to a high shear stress within an oscillatory flow system enhances platelet reactivity; this reaction may take part in the production of platelet and coagulation changes seen in the atherosclerotic patient and after strenuous physical exercise.

[1]  J. Latour,et al.  Potentiation by alpha and inhibition by beta-adrenergic stimulations of rat platelet aggregation. A comparative study with human and rabbit platelets. , 1977, Thrombosis and haemostasis.

[2]  S. Oka.  A theoretical approach to the effect of shear stress on the development of atheroma. , 1976, Thrombosis research.

[3]  H. Goldsmith,et al.  The effect of oscillatory flow on the release reaction and aggregation of human platelets. , 1976, Microvascular research.

[4]  H. Goldsmith,et al.  Fluid Mechanical Stress and the Platelet , 1975, Thrombosis and Haemostasis.

[5]  J D Hellums,et al.  Morphological, biochemical, and functional changes in human platelets subjected to shear stress. , 1975, The Journal of laboratory and clinical medicine.

[6]  H. Schmid-schönbein,et al.  A rheological method for the quantification of platelet aggregation (PA) in vitro and its kinetics under defined flow conditions. , 1975, Thrombosis research.

[7]  V. Turitto,et al.  Platelet interaction with subendothelium in a perfusion system: physical role of red blood cells. , 1975, Microvascular research.

[8]  H. Goldsmith,et al.  Behavior of model particles and blood cells at spherical obstructions in tube flow. , 1973, Microvascular research.

[9]  H. Baumgartner,et al.  The role of blood flow in platelet adhesion, fibrin deposition, and formation of mural thrombi. , 1973, Microvascular research.

[10]  J. Mustard,et al.  Preparation of Suspensions of Washed Platelets from Humans , 1972, British journal of haematology.

[11]  F. Gunz.  Blood Microrheology—Viscosity Factors in Blood Flow, Ischaemia and Thrombosis , 1972 .

[12]  J S Lee,et al.  Flow in nonuniform small blood vessels. , 1971, Microvascular research.

[13]  R. Schroter,et al.  Atheroma and arterial wall shear - Observation, correlation and proposal of a shear dependent mass transfer mechanism for atherogenesis , 1971, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[14]  T. Shimamoto,et al.  Enhancement of ADP-Induced Platelet Aggregation by Exercise Test in Coronary Patients and Its Prevention by Pyridinolcarbamate , 1970, Thrombosis and Haemostasis.

[15]  D. L. Fry.  Certain Histological and Chemical Responses of the Vascular Interface to Acutely Induced Mechanical Stress in the Aorta of the Dog , 1969, Circulation research.

[16]  D J Patel,et al.  Application of Heated‐Film Velocity and Shear Probes to Hemodynamic Studies , 1968, Circulation research.

[17]  D. L. Fry.  Acute Vascular Endothelial Changes Associated with Increased Blood Velocity Gradients , 1968, Circulation research.

[18]  J. A. Fox,et al.  Localization of atheroma: a theory based on boundary layer separation. , 1966, British heart journal.

[19]  E. Ikkala,et al.  Platelet adhesiveness and ADP-induced platelet aggregation in exercise. , 1966, Annales medicinae experimentalis et biologiae Fenniae.

[20]  M Helpern,et al.  The role of vascular dynamics in the development of atherosclerosis. , 1965, JAMA.

[21]  P. Sawyer,et al.  THE SIGNIFICANCE OF TURBULENCE IN HEMIC SYSTEMS AND IN THE DISTRIBUTION OF THE ATHEROSCLEROTIC LESION. , 1965, Surgery.

[22]  R. Aster,et al.  PLATELET SEQUESTRATION IN MAN. I. METHODS. , 1964, The Journal of clinical investigation.

[23]  P. Jennings,et al.  Dietary fat and platelet adhesiveness in arteriosclerosis and diabetes , 1963 .

[24]  H. Rowsell,et al.  Encrustation and atherosclerosis: the analogy between early in vivo lesions and deposits which occur in extracorporeal circulations. , 1962, Canadian Medical Association journal.

[25]  G. Born,et al.  Aggregation of Blood Platelets by Adenosine Diphosphate and its Reversal , 1962, Nature.

[26]  J. Mustard,et al.  Coagulation Tests and Platelet Economy in Atherosclerotic and Control Subjects , 1962, Circulation.

[27]  R B HUNTER,et al.  ARTERIAL DISEASE , 1977, Bibliotheca cardiologica.

[28]  M. Texon,et al.  A hemodynamic concept of atherosclerosis, with particular reference to coronary occlusion. , 1957, A.M.A. archives of internal medicine.