Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity

A culture supernatant of concanavalin A-activated spleen cells (Con A supernatant) induced murine macrophages to express Ia antigens in vitro. Biochemical characterization of the Con A supernatant indicated that the macrophage Ia antigen regulatory activity shares molecular weight, pI, and hydrophobic and affinity characteristics with immune interferon (IFN-gamma). Antiserum to mouse IFN-gamma neutralized both the macrophage Ia antigen regulatory and IFN-gamma bioactivities of the Con A supernatant. Furthermore, both partially purified murine IFN- gamma (10(7) U/mg protein sp act) and IFN-containing culture supernatants of the murine BFS T cell line-induced macrophage Ia antigen expression in vitro. Culture supernatants containing colony- stimulating factor, interleukin 1, interleukin 2, macrophage migration inhibitory factor, and a macrophage-activating activity that were distinct from IFN-gamma did not induce macrophage Ia antigen expression. Taken together, the data indicate that the in vitro expression of Ia antigens on macrophages is regulated by an activity that has the characteristics of interferon.

[1]  P. Steeg,et al.  Production of immune interferon by an interleukin 2-independent murine T cell line. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[2]  L. Lanier,et al.  Concomitant induction of the cell surface expression of Ia determinants and accessory cell function by a murine macrophage tumor cell line , 1982, The Journal of experimental medicine.

[3]  V. Kelley.  Increase in ia positive macrophages in mrl autoimmune mice. Abstr. , 1982 .

[4]  D. Green,et al.  The induction of autoimmunity in normal mice. Abstr. , 1982 .

[5]  M. Hilfiker,et al.  Biologic properties of chromatographically separated murine thymoma-derived Interleukin 2 and colony-stimulating factor. , 1981, Journal of immunology.

[6]  B. Stadler,et al.  Epidermal cell (keratinocyte)-derived thymocyte-activating factor (ETAF). , 1981, Journal of immunology.

[7]  Z. Cohn,et al.  Studies of the cell surface of mouse dendritic cells and other leukocytes , 1981, The Journal of experimental medicine.

[8]  W. Farrar,et al.  Regulation of the production of immune interferon and cytotoxic T lymphocytes by interleukin 2. , 1981, Journal of immunology.

[9]  M. Meltzer.  CYTOLYSIS OF TUMOR CELLS BY RELEASE OF [3H−] THYMIDINE , 1981 .

[10]  P. Steeg,et al.  Regulation of murine macrophage Ia-antigen expression by products of activated spleen cells , 1980, The Journal of experimental medicine.

[11]  R. Steinman,et al.  Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages , 1980, The Journal of experimental medicine.

[12]  W. Stewart,et al.  Production, partial purification and characterization of human and murine interferons--type II. , 1980, Molecular immunology.

[13]  E. Unanue,et al.  IA antigens and antigen-presenting function of thymic macrophages. , 1980, Journal of immunology.

[14]  J. Berzofsky,et al.  The murine Kupffer cell. I. Characterization of the cell serving accessory function in antigen-specific T cell proliferation. , 1979, Journal of immunology.

[15]  S. Pincus,et al.  A subpopulation of adherent accessory cells bearing both I-A and I-E or C subregion antigens is required for antigen-specific murine T lymphocyte proliferation. , 1978, Journal of immunology.

[16]  A. Singer,et al.  Cellular and genetic control of antibody responses in vitro. IV. Expression of Ia antigens on accessory cells required for responses to soluble antigens including a response under Ir gene control. , 1978, Journal of immunology.

[17]  B. Schwartz,et al.  Macrophage Ia antigens. I. macrophage populations differ in their expression of Ia antigens. , 1978, Journal of immunology.

[18]  E. Unanue,et al.  SUBPOPULATIONS OF PERITONEAL MACROPHAGES IDENTIFIED WITH ANTI-Ia SERA , 1978 .

[19]  H. Koren,et al.  Identification of macrophage-like characteristics in a cultured murine tumor line. , 1975, Journal of immunology.

[20]  K. Cantell,et al.  No evidence for a carbohydrate moiety affecting the clearance of circulating human leukocyte interferon in rabbits. , 2009, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology.

[21]  B. Pike,et al.  Human bone marrow colony growth in agar‐gel , 1970, Journal of cellular physiology.

[22]  R. Wagner.  Biological studies of interferon. I. Suppression of cellular infection with eastern equine encephalomyelitis virus. , 1961, Virology.