A preparation of a single-layered enzyme-membrane using asymmetric pBPPO base film for development of pesticide detecting biosensor

Abstract A novel single-layered enzyme-membrane using BPPO film which was pore-filled with cross-linked PVA containing TYR was prepared to develop a pesticide biosensor with an increased stability. The prepared enzyme-membrane was assembled on a glassy carbon electrode to detect pesticides. The voltammetric measurements revealed a coupled reaction, an enzymatic oxidation and an electrochemical reduction of catechol transported through the prepared membrane. A detection range of parathion and carbaryl was 0.01–1 ppb and 0.01–10 ppb, respectively. The activity in the enzyme-membrane was maintained for 1 month due to favorable aqueous environment of PVA for enzyme activity while BPPO film provided structural stability.

[1]  Ursula Bilitewski,et al.  Enzyme electrodes for the determination of carbohydrates in food , 1993 .

[2]  T. Rinken,et al.  Transient phase calibration of tyrosinase-based carbaryl biosensor , 2004 .

[3]  S. Upadhyay,et al.  Membrane permeability based cholesterol sensor — A new possibility , 2000 .

[4]  S. Nakao,et al.  Pore-filling type polymer electrolyte membranes for a direct methanol fuel cell , 2003 .

[5]  J. Vidal,et al.  A comparative study of immobilization methods of a tyrosinase enzyme on electrodes and their application to the detection of dichlorvos organophosphorus insecticide. , 2006, Talanta.

[6]  N. Hampp,et al.  Comparison of potentiometric enzyme sensors for urea and penicillin-G: differential thick-film sensors versus classical electrodes , 1995 .

[7]  P. Vadgama.  Membrane based sensors: A review☆ , 1990 .

[8]  Shuguang Zhang.  Hydrogels: Wet or let die. , 2004, Nature materials.

[9]  S. Nakao,et al.  Polymer Electrolyte Membranes with a Pore‐Filling Structure for a Direct Methanol Fuel Cell , 2003 .

[10]  Lucas Franco Ferreira,et al.  Amperometric biosensing of carbamate and organophosphate pesticides utilizing screen-printed tyrosinase-modified electrodes. , 2007, Analytica chimica acta.

[11]  Richard P. Buck,et al.  Microfabricated amperometric creatine and creatinine biosensors , 1996 .

[12]  L. Campanella,et al.  Organophosphorus and carbamate pesticide analysis using an inhibition tyrosinase organic phase enzyme sensor; comparison by butyrylcholinesterase+choline oxidase opee and application to natural waters. , 2007, Analytica chimica acta.

[13]  Seung-Hyeon Moon,et al.  Optimized coverage of gold nanoparticles at tyrosinase electrode for measurement of a pesticide in various water samples. , 2008, Journal of hazardous materials.

[14]  Dan Wu,et al.  Preparation and characterization of CPPO/BPPO blend membranes for potential application in alkaline direct methanol fuel cell , 2008 .

[15]  N. Li,et al.  A Penicillamine Biosensor Based on Tyrosinase Immobilized on Nano-Au/ PAMAM Dendrimer Modified Gold Electrode , 2007 .

[16]  T. Xu,et al.  Fundamental studies of a new series of anion exchange membranes: membrane preparation and characterization , 2001 .

[17]  Guodong Liu,et al.  Amperometric tyrosinase biosensor based on Fe3O4 nanoparticles-chitosan nanocomposite. , 2008, Biosensors & bioelectronics.

[18]  Seung-Hyeon Moon,et al.  Preparation of a highly sensitive enzyme electrode using gold nanoparticles for measurement of pesticides at the ppt level. , 2008, Journal of environmental monitoring : JEM.

[19]  S. S. Mahajan.  Structural Modification of Poly(2,6-Dimethyl-1,4-Phenylene Oxide) , 1991 .

[20]  P. Vadgama,et al.  Engineering the right membranes for electrodes at the biological interface; solvent cast and electropolymerised. , 1995, Biosensors & bioelectronics.

[21]  S. Turmanova,et al.  Radiation grafting of acrylic acid onto polytetrafluoroethylene films for glucose oxidase immobilization and its application in membrane biosensor , 1997 .

[22]  G. Palleschi,et al.  Enzyme inhibition-based biosensors for food safety and environmental monitoring. , 2006, Biosensors & bioelectronics.

[23]  Pierre R. Coulet,et al.  Polymeric membranes and coupled enzymes in the design of biosensors , 1992 .

[24]  L. Fonseca,et al.  Applications of polymers for biomolecule immobilization in electrochemical biosensors , 2008 .

[25]  Dan Wu,et al.  A novel proton-conductive membrane with reduced methanol permeability prepared from bromomethylated poly(2,6-dimethyl-1,4 -phenylene oxide) (BPPO) , 2008 .