Evidence that the ciliary inhibitor of cystic fibrosis is not an antibody.

An inhibitor of ciliary activity is present in the serum of cystic fibrosis (CF) patients and heterozygotes. Fractionation of CF serum has indicated that the inhibitor is associated with the serum IgG fraction. This study compared the activity of the CF inhibitor to that of rabbit antibody preparations directed against oyster cilia. The aim of this investigation was to determine whether the ciliary inhibitory mechanism in cystic fibrosis is related to a typical immunological reaction. Results from experiments utilizing fluorescent antibody techniques demonstrated that an antiserum directed against oyster ciliated epithelium binds immunologically with both human bronchial and oyster epithelial tissue. Results from experiments utilizing immunofluorescent tracing, passive hemagglutination, double immunodiffusion, and papain digestion, however, indicated that the interaction between the oyster cilia and the CF serum inhibitor was not a typical antigen-antibody reaction.

[1]  R. Matalon,et al.  Cystic fibrosis: fractionation of fibroblast media demonstrating ciliary inhibition. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[2]  I. Schmoyer,et al.  Fractionation of oyster cilia inhibitor from cystic fibrosis heterozygote serum. , 1972, Biochemical and biophysical research communications.

[3]  T. The,et al.  Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method. , 1970, Immunology.

[4]  M. McCombs,et al.  Cystic Fibrosis: Characterization of the Inhibitor to Ciliary Action in Oyster Gills , 1970, Science.

[5]  R. Williams,et al.  Differences in anti-protein A activity among IgG subgroups. , 1969, Journal of immunology.

[6]  G. Besley,et al.  Inhibition of the motility of gill cilia of Dreissensia by plasma of cystic fibrosis patients and their parents. , 1969, Journal of medical genetics.

[7]  M. McCombs,et al.  Oyster Ciliary Inhibition by Cystic Fibrosis Factor , 1969, Science.

[8]  R. C. Nairn.  Fluorescent protein tracing , 1969 .

[9]  M. Joniau,et al.  Glutaraldehyde as a coupling reagent in passive haemagglutination. , 1969, Immunology.

[10]  J. Mangos,et al.  Studies on the Mechanism of Inhibition of Sodium Transport in Cystic Fibrosis of the Pancreas , 1968, Pediatric Research.

[11]  P. di Sant'Agnese,et al.  Pathogenesis and physiopathology of cystic fibrosis of the pancreas. Fibrocystic disease of the pancreas (Mucoviscidosis). , 1967, The New England journal of medicine.

[12]  J. Mangos,et al.  Sodium Transport: Inhibitory Factor in Sweat of Patients with Cystic Fibrosis , 1967, Science.

[13]  H. Heick,et al.  Abnormal Serum Factor in Patients with Cystic Fibrosis of the Pancreas , 1967, Pediatric Research.

[14]  P. Piggot,et al.  The N- and c-terminal amino acid sequences of the heavy chain from a pathological human immunoglobulin IgG. , 1966, The Biochemical journal.

[15]  C. Yonge.  The American Oyster , 1965, Nature.

[16]  A. Bearn,et al.  THE PRESENCE OF SUBUNITS IN THE INHERITED GROUP-SPECIFIC PROTEIN OF HUMAN SERUM. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[17]  O OUCHTERLONY,et al.  Diffusion-in-gel methods for immunological analysis. , 1958, Progress in allergy.

[18]  H. Kacser,et al.  Studies on the antigens of Paramecium aurelia with the aid of fluorescent antibodies. , 1957, Journal of general microbiology.

[19]  J. Scheidegger.  Une micro-méthode de l’immuno-électrophorèse , 1955 .

[20]  H. A. Sober,et al.  Chromatography of proteins on cellulose ion-exchangers. , 1954 .

[21]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[22]  R. A. Kekwick.  The serum proteins in multiple myelomatosis. , 1940, The Biochemical journal.