Mitochondria are the main target for oxidative damage in leaves of wheat (Triticum aestivum L.).

Photosynthesis, respiration, and other processes produce reactive oxygen species (ROS) that can cause oxidative modifications to proteins, lipids, and DNA. The production of ROS increases under stress conditions, causing oxidative damage and impairment of normal metabolism. In this work, oxidative damage to various subcellular compartments (i.e. chloroplasts, mitochondria, and peroxisomes) was studied in two cultivars of wheat differing in ascorbic acid content, and growing under good irrigation or drought. In well-watered plants, mitochondria contained 9-28-fold higher concentrations of oxidatively modified proteins than chloroplasts or peroxisomes. In general, oxidative damage to proteins was more intense in the cultivar with the lower content of ascorbic acid, particularly in the chloroplast stroma. Water stress caused a marked increase in oxidative damage to proteins, particularly in mitochondria and peroxisomes. These results indicate that mitochondria are the main target for oxidative damage to proteins under well-irrigated and drought conditions.

[1]  A. Millar,et al.  Control of Ascorbate Synthesis by Respiration and Its Implications for Stress Responses1 , 2003, Plant Physiology.

[2]  R. Mittler.  Oxidative stress, antioxidants and stress tolerance. , 2002, Trends in plant science.

[3]  S. Driscoll,et al.  Drought and oxidative load in the leaves of C3 plants: a predominant role for photorespiration? , 2002, Annals of botany.

[4]  G. Pastori,et al.  Common Components, Networks, and Pathways of Cross-Tolerance to Stress. The Central Role of “Redox” and Abscisic Acid-Mediated Controls1 , 2002, Plant Physiology.

[5]  Alex Levine,et al.  Oxidative Stress Increased Respiration and Generation of Reactive Oxygen Species, Resulting in ATP Depletion, Opening of Mitochondrial Permeability Transition, and Programmed Cell Death1 , 2002, Plant Physiology.

[6]  A. S. Raghavendra,et al.  Essentiality of Mitochondrial Oxidative Metabolism for Photosynthesis: Optimization of Carbon Assimilation and Protection Against Photoinhibition , 2002, Critical reviews in biochemistry and molecular biology.

[7]  P. M. Neumann,et al.  Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. , 2001, Journal of experimental botany.

[8]  Russell L. Jones,et al.  Signalling in the cereal aleurone: hormones, reactive oxygen and cell death. , 2001, The New phytologist.

[9]  C. Foyer,et al.  Plant antioxidants: colour me healthy. , 2001, Biologist.

[10]  I. Møller.  PLANT MITOCHONDRIA AND OXIDATIVE STRESS: Electron Transport, NADPH Turnover, and Metabolism of Reactive Oxygen Species. , 2001, Annual review of plant physiology and plant molecular biology.

[11]  F. J. Corpas,et al.  Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells. , 2001, Trends in plant science.

[12]  J. Whelan,et al.  Unraveling the Role of Mitochondria During Oxidative Stress in Plants , 2001, IUBMB life.

[13]  I. Graham,et al.  Stress induces peroxisome biogenesis genes , 2000, The EMBO journal.

[14]  N. Smirnoff,et al.  Ascorbic Acid in Plants: Biosynthesis and Function , 2000, Critical reviews in biochemistry and molecular biology.

[15]  G. Pastori,et al.  Ascorbate biosynthesis in mitochondria is linked to the electron transport chain between complexes III and IV. , 2000, Plant physiology.

[16]  J. Araus,et al.  Oxidative damage to thylakoid proteins in water-stressed leaves of wheat (Triticum aestivum). , 2000 .

[17]  D. Daley,et al.  Regulatory, Developmental and Tissue Aspects of Mitochondrial Biogenesis in Plants , 2000 .

[18]  J. Kangasjärvi,et al.  Short communication: subcellular localization of ozone-induced hydrogen peroxide production in birch (Betula pendula) leaf cells. , 1999, The Plant journal : for cell and molecular biology.

[19]  C. Foyer,et al.  Acclimation of photosynthesis, H2O2 content and antioxidants in maize (Zea mays ) grown at sub-optimal temperatures , 1999 .

[20]  K. Niyogi,et al.  PHOTOPROTECTION REVISITED: Genetic and Molecular Approaches. , 1999, Annual review of plant physiology and plant molecular biology.

[21]  E. Tambussi,et al.  Drought and watering-dependent oxidative stress: effect on antioxidant content in Triticum aestivum L. leaves , 1999 .

[22]  G. Pastori,et al.  Role of the ascorbate-glutathione cycle of mitochondria and peroxisomes in the senescence of pea leaves , 1998, Plant physiology.

[23]  C. Foyer,et al.  ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control. , 1998, Annual review of plant physiology and plant molecular biology.

[24]  D. Day,et al.  Alternative solutions to radical problems , 1997 .

[25]  A. Purvis.  Role of the alternative oxidase in limiting superoxide production by plant mitochondria , 1997 .

[26]  F. Navari-Izzo,et al.  Sunflower seedlings subjected to increasing stress by water deficit: Changes in O2− production Aated to the composition of thylakoid membranes , 1996 .

[27]  T. K. Prasad,et al.  Acclimation, Hydrogen Peroxide, and Abscisic Acid Protect Mitochondria against Irreversible Chilling Injury in Maize Seedlings , 1994, Plant physiology.

[28]  Christine H. Foyer,et al.  Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants , 1993 .

[29]  F. J. Corpas,et al.  Salt-induced oxidative stress mediated by activated oxygen species in pea leaf mitochondria , 1993 .

[30]  G. Beakes,et al.  Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons , 1985, Planta.

[31]  W. Inskeep,et al.  Extinction coefficients of chlorophyll a and B in n,n-dimethylformamide and 80% acetone. , 1985, Plant physiology.

[32]  P. Mullineaux,et al.  Oxygen Metabolism and the Regulation of Photosynthetic Electron Transport , 2019, Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants.

[33]  B. Halliwell.  Photorespiration , 2017, Methods in Molecular Biology.

[34]  W. Kaniewski,et al.  Isolation and Purification , 2001 .

[35]  C. Foyer,et al.  Bundle sheath proteins are more sensitive to oxidative damage than those of the mesophyll in maize leaves exposed to paraquat or low temperatures. , 2000, Journal of experimental botany.

[36]  T. Sharkey,et al.  Photosynthesis : physiology and metabolism , 2000 .

[37]  J. Graham,et al.  Subcellular fractionation : a practical approach , 1997 .

[38]  E. Stadtman,et al.  Carbonyl assays for determination of oxidatively modified proteins. , 1994, Methods in enzymology.

[39]  E. Elstner,et al.  Mechanisms of oxygen activation during plant stress , 1994 .

[40]  M. Salin.  Chloroplast and mitochondrial mechanisms for protection against oxygen toxicity. , 1991, Free radical research communications.