Dietary effects on glycogen and lipoprotein lipase activity in skeletal muscle in man.

The influence of short-term adaptation to a fat and protein enriched diet (F + P) and a carbohydrate enriched diet (CHO) on skeletal muscle lipoprotein lipase (LPL) activity and muscle glycogen levels was evaluated in 7 males. Muscle biopsies were taken from the m. vastus lateralis after an uncontrolled, mixed diet (M), after a 3 day F + P) diet preceded by intense exercise, and after a 3 day CHO diet. After the F + P diet glycogen concentration was 55% that of the M diet while LPL activity increased by 21% (n.s.). After the CHO diet glycogen levels increased by 82% and LPL activity decreased by 55% compared to the M diet (p less than 0.01). The changes in LPL after the CHO diet were related to the changes in glycogen concentration (r = 0.98, p less than 0.01). LPL activity in the control situation was directly related to percent slow twitch (ST) muscle fibre type (r = 0.95, p less than 0.01). The results suggest that the uptake of fat from the circulation may be actively regulated by the muscle as a function of intramuscular substrate availability and that this regulation may be related to muscle fibre type composition.

[1]  I. Jacobs Lactate concentrations after short, maximal exercise at various glycogen levels. , 1981, Acta physiologica Scandinavica.

[2]  B. Saltin,et al.  Capillary supply and lipoprotein-lipase activity in skeletal muscle in man. , 1981, Acta physiologica Scandinavica.

[3]  G. Dudley,et al.  Uptake of chylomicron triglycerides by contracting skeletal muscle in rats. , 1980, Journal of applied physiology: respiratory, environmental and exercise physiology.

[4]  G. Dudley,et al.  Influence of thyroid status on skeletal muscle LPL activity and TG uptake. , 1980, The American journal of physiology.

[5]  M. Schotz,et al.  Rapid changes in rat heart lipoprotein lipase activity after feeding carbohydrate. , 1980, The Journal of nutrition.

[6]  M. Taskinen,et al.  Effect of acute vigorous exercise on lipoprotein lipase activity of adipose tissue and skeletal muscle in physically active men. , 1980, Artery.

[7]  J. Karlsson,et al.  Changes in lipoprotein-lipase activity and lipid stores in human skeletal muscle with prolonged heavy exercise. , 1979, Acta physiologica Scandinavica.

[8]  N. Christensen,et al.  The effect of different diets and of insulin on the hormonal response to prolonged exercise. , 1979, Acta physiologica Scandinavica.

[9]  J. Borensztajn Lipoprotein lipase and hypertriglyceridemias. , 1979, Artery.

[10]  M. Taskinen,et al.  Lipoprotein lipase activity in adipose tissue and skeletal muscle of runners: relation to serum lipoproteins. , 1978, Metabolism: clinical and experimental.

[11]  H. Lithell,et al.  Lipoprotein-lipase activity in human skeletal muscle and adipose tissue in the fasting and the fed states. , 1978, Atherosclerosis.

[12]  H. Lithell,et al.  Increase of the lipoprotein‐lipase activity in human skeletal muscle during clof ibrate administration , 1978, European journal of clinical investigation.

[13]  H. Lithell,et al.  Determination of lipoprotein-lipase activity in human skeletal muscle tissue. , 1978, Biochimica et biophysica acta.

[14]  A Thorstensson,et al.  Maximal oxygen uptake and muscle fiber types in trained and untrained humans. , 1978, Medicine and science in sports.

[15]  J. Henriksson,et al.  Capillary supply of the quadriceps femoris muscle of man: adaptive response to exercise , 1977, The Journal of physiology.

[16]  P. Nilsson-ehle,et al.  A stable, radioactive substrate emulsion for assay of lipoprotein lipase. , 1976, Journal of lipid research.

[17]  J. Borensztajn,et al.  Effect of exercise on lipoprotein lipase activity in rat heart and skeletal muscle. , 1975, The American journal of physiology.

[18]  C. Delorme,et al.  Effects of diet on lipoprotein lipase activity in the rat. , 1975, The Journal of nutrition.

[19]  B. Saltin,et al.  Muscle glycogen utilization during exercise after physical training. , 1974, Acta physiologica Scandinavica.

[20]  O. H. Lowry CHAPTER 8 – ENZYMATIC CYCLING , 1972 .

[21]  B. Saltin,et al.  Availability of substrates and capacity for prolonged heavy exercise in man. , 1971, Journal of applied physiology.

[22]  M. Brooke,et al.  THREE "MYOSIN ADENOSINE TRIPHOSPHATASE" SYSTEMS: THE NATURE OF THEIR pH LABILITY AND SULFHYDRYL DEPENDENCE , 1970, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[23]  E. Shafrir,et al.  Effect of Experimental Hypertriglyceridaemia on Tissue and Serum Lipoprotein Lipase Activity , 1970, European journal of clinical investigation.

[24]  H. A. Padykula,et al.  THE SPECIFICITY OF THE HISTOCHEMICAL METHOD FOR ADENOSINE TRIPHOSPHATAS , 1955, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[25]  E. H. Christensen,et al.  V. Respiratorischer Quotient und O2‐Aufnahme1 , 1939 .

[26]  E. Hohwü Christensen,et al.  III. Arbeitsfähigkeit und Ernährung1 , 1939 .