Hydrogen Peroxide Sensor Based on Hemoglobin Immobilized on Glassy Carbon Electrode with SiO2 Nanoparticles/Chitosan Film as Immobilization Matrix

Abstract A novel amperometric sensor of hydrogen peroxide was constructed. Hemoglobin (Hb) was successfully immobilized on nanometer‐sized SiO2, which was supported by chitosan. Chitosan was acted as dispersant. The determination of hydrogen peroxide was performed in the presence of an electron mediator hydroquinone. Hb immobilized on the SiO2/chitosan composite film displayed excellent electrocatalytical activity to the reduction of H2O2. The linear range of detection towards H2O2 was from 6.25×10−7 to 1.63×10−4mol/L with a detection limit of 1.8×10−7mol/L (S/N=3). The apparent Michaelis‐Menten constant (K app M) was found to be 0.75mmol/L.

[1]  H. Ju,et al.  Preparation of porous titania sol-gel matrix for immobilization of horseradish peroxidase by a vapor deposition method. , 2002, Analytical chemistry.

[2]  N. Pieczonka,et al.  Gold nanoparticle embedded, self-sustained chitosan films as substrates for surface-enhanced Raman scattering. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[3]  Jing‐Juan Xu,et al.  Synergistic effect of zirconium phosphate and Au nanoparticles on direct electron transfer of hemoglobin on glassy carbon electrode , 2005 .

[4]  Huangxian Ju,et al.  Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayer , 1999 .

[5]  Jing‐Juan Xu,et al.  Electrochemically deposited nanocomposite of chitosan and carbon nanotubes for biosensor application. , 2005, Chemical communications.

[6]  Manming Yan,et al.  Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in sol–gel-derived tin oxide/gelatin composite films , 2005 .

[7]  J. Rusling,et al.  Heme proteins sequestered in silica sol–gels using surfactants feature direct electron transfer and peroxidase activity , 2004 .

[8]  D. Zhao,et al.  Electrochemistry and biosensing reactivity of heme proteins adsorbed on the structure-tailored mesoporous Nb2O5 matrix , 2004 .

[9]  Jing Chen,et al.  Direct electron transfer and bioelectrocatalysis of hemoglobin at a carbon nanotube electrode. , 2004, Analytical biochemistry.

[10]  James R. Durrant,et al.  Immobilisation and bioelectrochemistry of proteins on nanoporous TiO2 and ZnO films , 2001 .

[11]  Shen-ming Chen,et al.  The characterization and bioelectrocatalytic properties of hemoglobin by direct electrochemistry of DDAB film modified electrodes , 2004 .

[12]  Maogen Zhang,et al.  Carbon nanotube-chitosan system for electrochemical sensing based on dehydrogenase enzymes. , 2004, Analytical chemistry.

[13]  Guo-Li Shen,et al.  Immobilization of horseradish peroxidase to a nano-Au monolayer modified chitosan-entrapped carbon paste electrode for the detection of hydrogen peroxide. , 2003, Talanta.

[14]  Jing-Juan Xu,et al.  Glucose biosensor based on ENFET doped with SiO2 nanoparticles , 2004 .

[15]  G. S. Wilson,et al.  Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer , 1980 .

[16]  Douglas J. Moffatt,et al.  Fourier Self-Deconvolution: A Method for Resolving Intrinsically Overlapped Bands , 1981 .

[17]  Zhihui Dai,et al.  Immobilization of hemoglobin on zirconium dioxide nanoparticles for preparation of a novel hydrogen peroxide biosensor. , 2004, Biosensors & bioelectronics.

[18]  Yong J. Yuan,et al.  Natural Polymer-Based Sulfite Biosensor , 1998 .

[19]  Suxia Zhang,et al.  Covalent attachment of glucose oxidase to an Au electrode modified with gold nanoparticles for use as glucose biosensor. , 2005, Bioelectrochemistry.

[20]  Weihong Tan,et al.  Immobilization of oligonucleotides onto silica nanoparticles for DNA hybridization studies , 2002 .

[21]  Min Li,et al.  An electrochemical investigation of hemoglobin and catalase incorporated in collagen films. , 2005, Biochimica et biophysica acta.

[22]  T. Ohsaka,et al.  A facilitated electron transfer of copper--zinc superoxide dismutase (SOD) based on a cysteine-bridged SOD electrode. , 2002, Biochimica et biophysica acta.

[23]  Guo-Li Shen,et al.  A mediator-free phenol biosensor based on immobilizing tyrosinase to ZnO nanoparticles. , 2006, Analytical biochemistry.

[24]  D. Pang,et al.  Direct electrochemistry and electrocatalysis of heme-proteins entrapped in agarose hydrogel films. , 2004, Biosensors & bioelectronics.

[25]  S. Yao,et al.  Study of the immobilization of alcohol dehydrogenase on Au-colloid modified gold electrode by piezoelectric quartz crystal sensor, cyclic voltammetry, and electrochemical impedance techniques. , 2003, Journal of colloid and interface science.

[26]  Jing-Juan Xu,et al.  Direct electrochemistry and electrocatalysis of heme proteins immobilized on gold nanoparticles stabilized by chitosan. , 2005, Analytical biochemistry.

[27]  Jin-Ming Lin,et al.  Chemiluminescent flow sensor for H2O2 based on the decomposition of H2O2 catalyzed by cobalt(II)-ethanolamine complex immobilized on resin , 2001 .

[28]  H. Abruña,et al.  Dithiobissuccinimidyl propionate as an anchor for assembling peroxidases at electrodes surfaces and its application in a H2O2 biosensor. , 1999, Analytical chemistry.

[29]  Gang Wang,et al.  Amperometric hydrogen peroxide biosensor with sol-gel/chitosan network-like film as immobilization matrix. , 2003, Biosensors & bioelectronics.

[30]  S. Tan,et al.  Amperometric hydrogen peroxide biosensor with silica sol-gel/chitosan film as immobilization matrix , 2001 .