Excitation-wavelength dependence of multiphoton excitation of fluorophores of human skin in vivo

We present in vivo measurements of the excitation-wavelength dependence of the autofluorescence of major endogenous fluorophores of human skin with a multiphoton tomograph. For the investigation high-resolution multiphoton images at different depths inside the skin were recorded and the main fluorophores identified. In particular, for the autofluorescence of the fluorophores keratin, NAD(P)H, elastin and for the second-harmonic-generation light induced by collagen fibers clear trends are shown.

[1]  Karsten König,et al.  Multiphoton microscopy for the investigation of dermal penetration of nanoparticle-borne drugs. , 2006, The Journal of investigative dermatology.

[2]  I. Riemann,et al.  In vivo Drug Screening in Human Skin Using Femtosecond Laser Multiphoton Tomography , 2006, Skin Pharmacology and Physiology.

[3]  Watt W Webb,et al.  Interpreting second-harmonic generation images of collagen I fibrils. , 2005, Biophysical journal.

[4]  Bruce J Tromberg,et al.  Imaging coronary artery microstructure using second-harmonic and two-photon fluorescence microscopy. , 2004, Biophysical journal.

[5]  K. König,et al.  Two-photon microscopes and in vivo multiphoton tomographs--powerful diagnostic tools for tissue engineering and drug delivery. , 2006, Advanced drug delivery reviews.

[6]  Iris Riemann,et al.  High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. , 2003, Journal of biomedical optics.

[7]  A. Pena,et al.  Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy. , 2005, Optics express.

[8]  Wolfgang Becker,et al.  In vitro and in vivo imaging of xenobiotic transport in human skin and in the rat liver , 2008, Journal of biophotonics.

[9]  Hans Georg Breunig,et al.  Multiphoton excitation characteristics of cellular fluorophores of human skin in vivo. , 2010, Optics express.

[10]  K. Fujita.  [Two-photon laser scanning fluorescence microscopy]. , 2007, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.

[11]  Karsten König,et al.  In vivo assessment of human skin aging by multiphoton laser scanning tomography. , 2006, Optics letters.

[12]  Karsten König,et al.  Clinical multiphoton tomography and clinical two-photon microendoscopy , 2009, BiOS.

[13]  Watt W Webb,et al.  Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein. , 2002, Biophysical journal.

[14]  Karsten König,et al.  Sensitivity and specificity of multiphoton laser tomography for in vivo and ex vivo diagnosis of malignant melanoma. , 2009, The Journal of investigative dermatology.