Electrochemical Biosensing Platforms Using Phthalocyanine-Functionalized Carbon Nanotube Electrode

Iron-phthalocyanines (FePc) are functionalized at multi-walled carbon nanotubes (MWNTs) to remarkably improve the sensitivity toward hydrogen peroxide. We constructed a highly sensitive and selective glucose sensor on FePcMWNTs electrode based on the immobilization of glucose oxidase (GOD) on poly-o-aminophenol (POAP)electropolymerized electrode surface. SEM images indicate that GOD enzymes trapped in POAP film tend to deposit primarily on the curved tips and evenly disperse along the sidewalls. The resulting GOD @ POAP/FePc-MWNTs biosensor exhibits excellent performance for glucose with a rapid response (less than 8 s), a wide linear range (up to 4.0 � 10 � 3 M), low detection limits (2.0 � 10 � 7 M with a signal-to-noise of 3), a highly reproducible response (RSD of 2.6%), and long-term stability (120 days). Such characteristics may be attributed to the catalytic activity of FePc and carbon nanotube, permselectivity of POAP film, as well as the large surface area of carbon nanotube materials.

[1]  A. Nozad,et al.  Electrocatalytic Oxidation of Methanol at Lower Potentials on Glassy Carbon Electrode Modified by Platinum and Platinum Alloys Incorporated in Poly(o‐Aminophenol) Film , 2003 .

[2]  Yuehe Lin,et al.  Solubilization of carbon nanotubes by Nafion toward the preparation of amperometric biosensors. , 2003, Journal of the American Chemical Society.

[3]  A. Ciucu,et al.  Detection of pesticides using an amperometric biosensor based on ferophthalocyanine chemically modified carbon paste electrode and immobilized bienzymatic system. , 2003, Biosensors & bioelectronics.

[4]  T. Tatsuma,et al.  Peroxidase-incorporated polypyrrole membrane electrodes , 1992 .

[5]  Jing‐Juan Xu,et al.  Amperometric glucose sensor based on glucose oxidase immobilized in electrochemically generated poly(ethacridine) , 2000 .

[6]  Joseph Wang,et al.  Carbon nanotube/teflon composite electrochemical sensors and biosensors. , 2003, Analytical chemistry.

[7]  G. Xu,et al.  Synthesis of vertically aligned carbon nanotubes films on silicon wafers by pyrolysis of ethylenediamine , 2002 .

[8]  Ray H. Baughman,et al.  Direct electron transfer of glucose oxidase on carbon nanotubes , 2002 .

[9]  A. Lever,et al.  Phthalocyanines : properties and applications , 1989 .

[10]  Q. Cai,et al.  Electron transfer on an electrode of glucose oxidase immobilized in polyaniline , 1994 .

[11]  Jun Li,et al.  Novel Three-Dimensional Electrodes: Electrochemical Properties of Carbon Nanotube Ensembles , 2002 .

[12]  C Hong-Yuan,et al.  Amperometric glucose sensor based on coimmobilization of glucose oxidase and Poly(p-phenylenediamine) at a platinum microdisk electrode. , 2000, Analytical biochemistry.

[13]  E. Wang,et al.  Direct electrochemistry and surface characterization of glucose oxidase adsorbed on anodized carbon electrodes , 1994 .

[14]  M. Koudelka-Hep,et al.  Nickel tetrasulfonated phthalocyanine based platinum microelectrode array for nitric oxide oxidation , 2002 .

[15]  Zhongping Huang,et al.  Electrochemical synthesis of polypyrrole films over each of well-aligned carbon nanotubes , 2001 .

[16]  G. Rivas,et al.  Glucose Biosensor Prepared by the Deposition of Iridium and Glucose Oxidase on Glassy Carbon Transducer , 1999 .

[17]  Daoben Zhu,et al.  Immobilization of tetra-tert-butylphthalocyanines on carbon nanotubes: a first step towards the development of new nanomaterials , 2002 .

[18]  Qi Wang,et al.  Properties, and Applications , 2005 .

[19]  J. Deng,et al.  A Glucose Biosensor Based on Immobilization of Glucose Oxidase in Electropolymerized o-Aminophenol Film on Platinized Glassy Carbon Electrode. , 1996, Analytical chemistry.

[20]  N. Oyama,et al.  Preparation of thin polymeric films on electrode surfaces by electropolymerization of o-aminophenol , 1988 .

[21]  J. Bockris,et al.  On the Adsorption of Glucose Oxidase at a Gold Electrode , 1989 .

[22]  J. Oni,et al.  Voltammetric Detection of Vitamin B1 at Carbon Paste Electrodes and Its Determination in Tablets , 2002 .

[23]  Pulickel M. Ajayan,et al.  Fast Electron Transfer Kinetics on Multiwalled Carbon Nanotube Microbundle Electrodes , 2001 .

[24]  W. C. Tjiu,et al.  Growth of vertically aligned carbon-nanotube array on large area of quartz plates by chemical vapor deposition , 2002 .

[25]  Pulickel M. Ajayan,et al.  EFFICIENT POLYMERIZATION OF ANILINE AT CARBON NANOTUBE ELECTRODES , 1999 .

[26]  J. Ahn,et al.  Multi-walled carbon nanotubes for the immobilization of enzyme in glucose biosensors , 2003 .

[27]  C. Lowe,et al.  Immobilization of glucose oxidase in ferrocene-modified pyrrole polymers. , 1988, Analytical chemistry.

[28]  J. Luong,et al.  Electrochemical biosensing platforms using platinum nanoparticles and carbon nanotubes. , 2004, Analytical chemistry.

[29]  S. Dong,et al.  Flow-injection analysis of glucose at an amperometric glucose sensor based on electrochemical deposition of palladium and glucose oxidase on a glassy carbon electrode , 1993 .

[30]  Wei‐De Zhang,et al.  Direct Electron Transfer of Glucose Oxidase Molecules Adsorbed onto Carbon Nanotube Powder Microelectrode , 2002, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[31]  D. Tonelli,et al.  Electrocatalytic activity of cobalt phthalocyanine stabilized by different matrixes , 2002, Analytical and bioanalytical chemistry.

[32]  J. Lee,et al.  Synthesis and electrochromic properties of poly-o-aminophenol , 1994 .

[33]  S. Iijima.  Helical microtubules of graphitic carbon , 1991, Nature.

[34]  Fwu-Shan Sheu,et al.  Selective Voltammetric Detection of Uric Acid in the Presence of Ascorbic Acid at Well‐Aligned Carbon Nanotube Electrode , 2003 .

[35]  Hongyuan Chen,et al.  An Amperometric Biosensor Based on the Coimmobilization of Horseradish Peroxidase and Methylene Blue on a Carbon Nanotubes Modified Electrode , 2003 .

[36]  C. Malitesta,et al.  Glucose fast-response amperometric sensor based on glucose oxidase immobilized in an electropolymerized poly(o-phenylenediamine) film. , 1990, Analytical chemistry.

[37]  N. Oyama,et al.  Electrode kinetics of poly (o-aminophenol) film prepared by electro-oxidative polymerization of o-aminophenol and its electrochromic properties , 1988 .

[38]  L. Cao,et al.  Carbon‐Nanotube‐Templated Assembly of Rare‐Earth Phthalocyanine Nanowires , 2003 .

[39]  C. Barbero,et al.  Formation of a novel electroactive film by electropolymerization of ortho-aminophenol: Study of its chemical structure and formation mechanism. Electropolymerization of analogous compounds , 1989 .

[40]  Leong Ming Gan,et al.  Synthesis of vertically aligned carbon nanotubes on metal deposited quartz plates , 2002 .

[41]  Malcolm L. H. Green,et al.  Bioelectrochemical single-walled carbon nanotubes. , 2002, Journal of the American Chemical Society.

[42]  Z. Gu,et al.  Investigation of the electrochemical and electrocatalytic behavior of single-wall carbon nanotube film on a glassy carbon electrode. , 2001, Analytical chemistry.