A dynamic risk assessment model (FUMAgrain) of fumonisin synthesis by Fusarium verticillioides in maize grain in Italy

Abstract Fumonisin contamination of maize grain starts in the field. Forecasting Fusarium infection and fumonisin synthesis could allow operators in the field to control contamination during the growing season and to make the best agronomic decisions for high quality yields while respecting the limits imposed by the European Union. A research project to develop a decision support system for the control of field-phase fumonisin contamination began in Italy in 2003. This paper presents a preliminary version of the engine of the decision support tool: FUMAgrain, a dynamic risk assessment model developed with data from the north of Italy. The structure of FUMAgrain is based on the pathosystem formed by maize, F. verticillioides and Ostrinia nubilalis (European Corn Borer). The elements of the pathosystem are simulated by three sub-models: (i) maize development, (ii) F. verticillioides infection and fumonisin synthesis, (iii) European Corn Borer wounding activity on maize grain. Inputs to the model are (i) planting date, (ii) hourly meteorological data including temperature, relative humidity, wind speed and rain intensity, (iii) information on the phenological development of the hybrid planted (flowering and dry-down), and (iv) information about the chemical treatment against European Corn Borer. FUMAgrain gives an initial risk alert at the end of flowering based on the meteorological conditions during this phase. A second alert follows maturation when an assessment is made from (i) maize grain moisture, (ii) European Corn Borer damage to the ear, and (iii) fumonisin synthesis risk. Following calibration and validation with data FUMAgrain demonstrated its good capability to simulate fumonisin synthesis in maize grain in Italy (calibration: R 2  = 0.70; validation: R 2  = 0.71) and its usefulness for determining the optimal harvest date while respecting grain safety levels required by the international market and limiting moisture content, hence drying costs.

[1]  G. Munkvold,et al.  Importance of Different Pathways for Maize Kernel Infection by Fusarium moniliforme. , 1997, Phytopathology.

[2]  D. Aylor.  Chapter 8 – Dispersal in Time and Space: Aerial Pathogens , 1978 .

[3]  Anna Kuparinen,et al.  Mechanistic models for wind dispersal. , 2006, Trends in plant science.

[4]  L. Burgess,et al.  Survival of Fusarium moniliforme at controlled temperature and relative humidity , 1985 .

[5]  J. Ooka.  Wind and Rain Dispersal of Fusarium moniliforme in Corn Fields , 1977 .

[6]  W. Marasas,et al.  Discovery and occurrence of the fumonisins: a historical perspective. , 2001, Environmental health perspectives.

[7]  D. Bhatnagar,et al.  The mycotoxin factbook : food & feed topics , 2006 .

[8]  Vijay Singh,et al.  Measurement and Maintenance of Corn Quality , 2019, Corn.

[9]  G. Munkvold,et al.  Seed Transmission of Fusarium verticillioides in Maize Plants Grown Under Three Different Temperature Regimes. , 2007, Plant disease.

[10]  J. D'mello,et al.  A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins , 1999 .

[11]  M. Pascale,et al.  Fusarium and fumonisin occurrence in Argentinian corn at different ear maturity stages. , 1996 .

[12]  Optimal use of data in crop model development and validation , 1995 .

[13]  P. Walklate,et al.  The Role of Rain in Dispersal of Pathogen Inoculum , 1989 .

[14]  Miroslav Trnka,et al.  European Corn Borer life stage model: Regional estimates of pest development and spatial distribution under present and future climate , 2007 .

[15]  S. Marín,et al.  Water and temperature relations and microconidial germination of Fusarium moniliforme and Fusarium proliferatum from maize. , 1996, Canadian journal of microbiology.

[16]  P. E. Nelson.  Taxonomy and biology of Fusarium moniliforme , 1992, Mycopathologia.

[17]  A. Logrieco,et al.  Toxigenic Fusarium Species and Mycotoxins Associated with Maize Ear Rot in Europe , 2002, European Journal of Plant Pathology.

[18]  A. Pietri,et al.  Occurrence of mycotoxins and ergosterol in maize harvested over 5 years in Northern Italy , 2004, Food additives and contaminants.

[19]  R. D. Brazee,et al.  Application of the diffusion equation for modelling splash dispersal of point-source pathogens. , 1991, The New phytologist.

[20]  S. Marín,et al.  Two-dimensional profiles of fumonisin B1 production by Fusarium moniliforme and Fusarium proliferatum in relation to environmental factors and potential for modelling toxin formation in maize grain. , 1999, International journal of food microbiology.

[21]  Manjit S. Kang,et al.  Genetic analyses of grain-filling rate and duration in maize 1 Approved for publication by the direc , 1999 .

[22]  F. Rombouts,et al.  Modeling of the Bacterial Growth Curve , 1990, Applied and environmental microbiology.

[23]  R. Singh,et al.  Application of GAB model for water sorption isotherms of food products. , 1996 .

[24]  J. Debevere,et al.  Effect of water activity and temperature on growth and the relationship between fumonisin production and the radial growth of Fusarium verticillioides and Fusarium proliferatum on corn. , 2005, Journal of food protection.

[25]  Hamed K. Abbas,et al.  Aflatoxin and fumonisin contamination of corn (maize, Zea mays) hybrids in Arkansas , 2006 .

[26]  D. M. Hinton,et al.  Symptomless endophytic colonization of maize by Fusarium moniliforme , 1996 .

[27]  P. Dowd.  Insect Management to Facilitate Preharvest Mycotoxin Management , 2003 .

[28]  Hughes,et al.  Decision‐making and diagnosis in disease management , 1999 .

[29]  J. David Miller,et al.  Modeling effects of environment, insect damage, and Bt genotypes on fumonisin accumulation in maize in Argentina and the Philippines , 2005, Mycopathologia.

[30]  S. A. Watson,et al.  Corn: chemistry and technology. , 1987 .

[31]  R. Hellmich,et al.  Aggregation and Dispersal Behavior of Marked and Released European Corn Borer (Lepidoptera: Crambidae) Adults , 2001 .

[32]  D. Bolonhezi,et al.  Accumulation of fumonisins B1 and B2 in freshly harvested Brazilian commercial maize at three locations during two nonconsecutive seasons , 2004, Mycopathologia.

[33]  J. Miller,et al.  Daily and seasonal dynamics of airborne spores of Fusarium graminearum and other Fusarium species sampled over wheat plots , 2000 .

[34]  D. Melcion,et al.  Growth of Fusarium moniliforme and its biosynthesis of fumonisin B1 on maize grain as a function of different water activities , 1995, Letters in applied microbiology.

[35]  N. Magan,et al.  In vitro control of growth and fumonisin production by Fusarium verticillioides and F. proliferatum using antioxidants under different water availability and temperature regimes , 2002, Journal of applied microbiology.

[36]  James G. Horsfall,et al.  Plant Disease: An Advanced Treatise , 1978 .

[37]  R. Huggett.  Mathematical models in agriculture: France, J. and Thornley, J.H.M. London: Butterworths, 1984. 335 pp. £35 hardback , 1985 .

[38]  A. Reyneri,et al.  Relationships between Ostrinia nubilalis (Lepidoptera: Crambidae) feeding activity, crop technique and mycotoxin contamination of corn kernel in northwestern Italy , 2005 .

[39]  W. Marasas,et al.  Natural occurrence of Fusarium and subsequent fumonisin contamination in preharvest and stored maize in Benin, West Africa. , 2005, International journal of food microbiology.

[40]  J. Miller,et al.  Factors that affect the occurrence of fumonisin. , 2001, Environmental health perspectives.

[41]  S. Marín,et al.  Fumonisin-producing strains of Fusarium: a review of their ecophysiology. , 2004, Journal of food protection.

[42]  D. W. Stewart,et al.  A mathematical simulation of growth of fusarium in maize ears after artificial inoculation. , 2002, Phytopathology.

[43]  P. A. Leffelaar.  On Systems Analysis and Simulation of Ecological Processes with Examples in CSMP and FORTRAN , 1993, Current Issues in Production Ecology.

[44]  D. Sparks,et al.  Fusarium verticillioides dissemination among maize ears of field-grown plants , 2008 .

[45]  J A Swets,et al.  Measuring the accuracy of diagnostic systems. , 1988, Science.

[46]  K. Cardwell,et al.  The Effect of Endophytic Fusarium verticillioides on Infestation of Two Maize Varieties by Lepidopterous Stemborers and Coleopteran Grain Feeders. , 2002, Phytopathology.

[47]  T. Kuiper-Goodman.  Approaches to the risk analysis of mycotoxins in the food supply , 1999 .

[48]  A. Schaafsma.  Economic changes imposed by mycotoxins in food grains: case study of deoxynivalenol in winter wheat. , 2002, Advances in experimental medicine and biology.

[49]  S. Marín,et al.  SEM study of water activity and temperature effects on the initial growth of Aspergillus ochraceus, Alternaria alternata and Fusarium verticillioides on maize grain. Scanning electron microscopy. , 2003, International journal of food microbiology.

[50]  P. Lancashire,et al.  A uniform decimal code for growth stages of crops and weeds , 1991 .

[51]  S. Marín,et al.  Water activity, temperature, and pH effects on growth of Fusarium moniliforme and Fusarium proliferatum isolates from maize. , 1995, Canadian journal of microbiology.

[52]  H. Njapau,et al.  Minimizing risks posed by mycotoxins utilizing the HACCP concept , 1999 .

[53]  B. Timlick,et al.  PHENOLOGY AND INFESTATION LEVEL OF THE EUROPEAN CORN BORER, OSTRINIA NUBILALIS (HÜBNER) (LEPIDOPTERA: PYRALIDAE), IN SOUTHERN MANITOBA , 1990, The Canadian Entomologist.

[54]  J. Hanway.  How a corn plant develops , 1966 .

[55]  J. Chełkowski,et al.  Mycotoxin contamination of maize hybrids after infection with Fusarium proliferatum , 1999 .

[56]  S. Marín,et al.  Fumonisin B1 Production and Growth of Fusarium moniliforme and Fusarium proliferatum on Maize, Wheat, and Barley Grain , 1999 .

[57]  S. Dragacci,et al.  Occurrence of fumonisins in foods , 2004 .

[58]  J P Flandrois,et al.  An unexpected correlation between cardinal temperatures of microbial growth highlighted by a new model. , 1993, Journal of theoretical biology.

[59]  V. Rossi,et al.  DYNAMICS OF AIRBORNE FUSARIUM MACROCONIDIA IN WHEAT FIELDS NATURALLY AFFECTED BY HEAD BLIGHT , 2002 .

[60]  H. Bleiholder,et al.  Explanations of the BBCH decimal codes for the growth stages of maize, rape, field beans, sunflower and peas -with illustrations , 1990 .

[61]  H. Boudra,et al.  Biotic and abiotic factors in fumonisin B1 production and stability , 1994 .

[62]  G. Munkvold,et al.  European Corn Borer (Lepidoptera: Pyralidae) Larvae as Vectors of Fusarium moniliforme, Causing Kernel Rot and Symptomless Infection of Maize Kernels , 1999 .

[63]  P. Murtaugh,et al.  The Statistical Evaluation of Ecological Indicators , 1996 .

[64]  V. Rossi,et al.  Modelling Fusarium verticillioides infection and fumonisin synthesis in maize ears , 2003 .

[65]  G. Munkvold,et al.  Comparison of Fumonisin Concentrations in Kernels of Transgenic Bt Maize Hybrids and Nontransgenic Hybrids. , 1999, Plant disease.

[66]  U. Siripatrawan,et al.  Determination of Moisture Sorption Isotherms of Jasmine Rice Crackers Using BET and GAB Models , 2006 .

[67]  G. Munkvold,et al.  Fumonisins in Maize: Can We Reduce Their Occurrence? , 1997, Plant disease.

[68]  A. Sharon,et al.  Early Events in the Fusarium verticillioides-Maize Interaction Characterized by Using a Green Fluorescent Protein-Expressing Transgenic Isolate , 2003, Applied and Environmental Microbiology.

[69]  David Makowski,et al.  Comparison of risk indicators for sclerotinia control in oilseed rape , 2005 .

[70]  Roberto Tuberosa,et al.  Conserved noncoding genomic sequences associated with a flowering-time quantitative trait locus in maize , 2007, Proceedings of the National Academy of Sciences.

[71]  A. Schaafsma,et al.  Climatic models to predict occurrence of Fusarium toxins in wheat and maize. , 2007, International journal of food microbiology.

[72]  G. Payne,et al.  Infection and Fumonisin Production by Fusarium verticillioides in Developing Maize Kernels. , 2004, Phytopathology.

[73]  G. Munkvold,et al.  Survival of Fusarium moniliforme, F. proliferatum, and F. subglutinans in Maize Stalk Residue. , 1998, Phytopathology.