Effects of 900 MHz electromagnetic fields exposure on cochlear cells' functionality in rats: Evaluation of distortion product otoacoustic emissions

In recent years, the widespread use of mobile phones has been accompanied by public debate about possible adverse consequences on human health. The auditory system is a major target of exposure to electromagnetic fields (EMF) emitted by cellular telephones; the aim of this study was the evaluation of possible effects of cellular phone-like emissions on the functionality of rat's cochlea. Distortion Products OtoAcoustic Emission (DPOAE) amplitude was selected as cochlea's outer hair cells (OHC) status indicator. A number of protocols, including different frequencies (the lower ones in rat's cochlea sensitivity spectrum), intensities and periods of exposure, were used; tests were carried out before, during and after the period of treatment. No significant variation due to exposure to microwaves has been evidenced.

[1]  K-A Hossmann,et al.  Effects of electromagnetic radiation of mobile phones on the central nervous system , 2003, Bioelectromagnetics.

[2]  Increased susceptibility of male rats to kanamycin-induced cochleotoxicity , 1999, Hearing Research.

[3]  Reilly Jp.  Comments concerning "Guidelines for limiting exposure to time-varying electric, magnetic, and electromagnetic fields (up to 300 GHz)". , 1999 .

[4]  W. Brownell,et al.  Micro- and nanomechanics of the cochlear outer hair cell. , 2001, Annual review of biomedical engineering.

[5]  James C Lin,et al.  Studies on microwaves in medicine and biology: From snails to humans , 2004, Bioelectromagnetics.

[6]  D. McFarland,et al.  Individual differences and the reliability of 2F1-F2 distortion-product otoacoustic emissions: effects of time-of-day, stimulus variables, and gender. , 1996, Journal of speech and hearing research.

[7]  A Basten,et al.  Lymphomas in E mu-Pim1 transgenic mice exposed to pulsed 900 MHZ electromagnetic fields. , 1997, Radiation research.

[8]  R Probst,et al.  A review of otoacoustic emissions. , 1991, The Journal of the Acoustical Society of America.

[9]  James C. Lin.  Health aspects of wireless communication: auditory perception of microwaves --- hearing microwaves , 2002, MOCO.

[10]  A. Guy,et al.  Auditory response in rats exposed to 2,450 MHz electromagnetic fields in a circularly polarized waveguide. , 1985, Bioelectromagnetics.

[11]  A. H. Frey.  Human auditory system response to modulated electromagnetic energy. , 1962, Journal of applied physiology.

[12]  J. Petruccelli,et al.  Evaluation of anesthesia effects in a rat animal model using otoacoustic emission protocols 1 1 Oral presentation at the Inner Ear Biology Workshop, Rome, 2001. , 2002, Hearing Research.

[13]  S. Hatzopoulos,et al.  Cisplatin Ototoxicity in the Sprague Dawley Rat Evaluated by Distortion Product Otoacoustic Emissions:Ototoxicidad por Cisplatino en la rata Sprague Dawley evaluada mediante productos de distorsión de las emisiones otoacústicas , 2001, Audiology : official organ of the International Society of Audiology.

[14]  R. Hamernik,et al.  The use of distortion product otoacoustic emissions in the estimation of hearing and sensory cell loss in noise-damaged cochleas , 2004, Hearing Research.

[15]  S. Manning,et al.  Middle Ear Pressure Variation: Effect of Nitrous Oxide , 1997, The Laryngoscope.

[16]  J. Puel,et al.  Development of 2ƒ1−ƒ2 otoacoustic emissions in the rat , 1987, Hearing Research.

[17]  C K Chou,et al.  Microwave effects on the nervous system , 2003, Bioelectromagnetics.

[18]  P. Avan,et al.  CD1 hearing-impaired mice. I: Distortion product otoacoustic emission levels, cochlear function and morphology , 1998, Hearing Research.

[19]  A. Kizilay,et al.  Effects of chronic exposure of electromagnetic fields from mobile phones on hearing in rats. , 2003, Auris, nasus, larynx.

[20]  M. Kundi,et al.  Mobile telephones and cancer: is there really no evidence of an association? (review). , 2003, International journal of molecular medicine.

[21]  Rudolf Probst,et al.  Repeatability of distortion product otoacoustic emissions in normally hearing humans. , 1993, Audiology : official organ of the International Society of Audiology.

[22]  R. Salvi,et al.  A review of the effects of selective inner hair cell lesions on distortion product otoacoustic emissions, cochlear function and auditory evoked potentials. , 2000, Noise & health.

[23]  W. R. Adey,et al.  Spontaneous and nitrosourea-induced primary tumors of the central nervous system in Fischer 344 rats chronically exposed to 836 MHz modulated microwaves. , 1999, Radiation research.

[24]  H. Lai,et al.  Acute low-intensity microwave exposure increases DNA single-strand breaks in rat brain cells. , 1995, Bioelectromagnetics.

[25]  W A Ahroon,et al.  Audiometric and histological differences between the effects of continuous and impulsive noise exposures. , 1993, The Journal of the Acoustical Society of America.

[26]  Val Gebski,et al.  Long-Term Exposure of Eμ-Pim1 Transgenic Mice to 898.4 MHz Microwaves does not Increase Lymphoma Incidence , 2002, Radiation research.

[27]  Pierre Campo,et al.  Use of DPOAEs for assessing hearing loss caused by styrene in the rat , 2002, Hearing Research.

[28]  K. Foster,et al.  Microwave Hearing: Evidence for Thermoacoustic Auditory Stimulation by Pulsed Microwaves , 1974, Science.

[29]  N. Harel,et al.  The effects of anesthesia on otoacoustic emissions , 1997, Hearing Research.

[30]  William E. Brownell,et al.  Outer Hair Cell Electromotility and Otoacoustic Emissions , 1990, Ear and hearing.

[31]  M. Repacholi.  Low-level exposure to radiofrequency electromagnetic fields: health effects and research needs. , 1998, Bioelectromagnetics.

[32]  G. M. Murray,et al.  The Effect of Nitrous Oxide on Hearing , 2000, Anaesthesia and intensive care.

[33]  D. Kemp.  Stimulated acoustic emissions from within the human auditory system. , 1978, The Journal of the Acoustical Society of America.

[34]  Orhan Ozturan,et al.  Effects of the Electromagnetic Field of Mobile Telephones on Hearing , 2002, Acta oto-laryngologica.

[35]  J. Elwood,et al.  Epidemiological studies of radio frequency exposures and human cancer , 2003, Bioelectromagnetics.

[36]  Z Sienkiewicz,et al.  Biological effects of electromagnetic fields and radiation , 1991 .

[37]  R. Seaman,et al.  Modification of acoustic startle by microwave pulses in the rat: a preliminary report. , 1992, Bioelectromagnetics.

[38]  H. Sohmer,et al.  Effect of high-dose cisplatin on auditory brainstem responses and otoacoustic emissions in laboratory animals. , 2000, The American journal of otology.

[39]  B. Veyret,et al.  No effect of short-term exposure to GSM-modulated low-power microwaves on benzo(a)pyrene-induced tumours in rat. , 1999, International journal of radiation biology.

[40]  A. Guy,et al.  Development of a rat head exposure system for simulating human exposure to RF fields from handheld wireless telephones. , 1999, Bioelectromagnetics.

[41]  C K Chou,et al.  Auditory response to pulsed radiofrequency energy , 2003, Bioelectromagnetics.

[42]  James C. Lin,et al.  MICROWAVE‐INDUCED ACOUSTIC EFFECTS IN MAMMALIAN AUDITORY SYSTEMS AND PHYSICAL MATERIALS * , 1975, Annals of the New York Academy of Sciences.

[43]  G. A. Lovisolo,et al.  Effects of microwaves (900 MHz) on the cochlear receptor: exposure systems and preliminary results , 2000, Radiation and environmental biophysics.