Preliminary Evaluation of Glyceric Acid-producing Ability of Acidomonas methanolica NBRC104435 from Glycerol Containing Methanol.

Some acetic acid bacteria produce large amounts of glyceric acid (GA) from glycerol in culture broth. However, methanol, which is a major contaminant of raw glycerol derived from the biodiesel fuel industry, sharply decreases cell growth and GA production [AMB Express, 3, 20, 2013]. Thus, we evaluated the methylotrophic acetic acid bacterium Acidomonas methanolica NBRC104435 for its ability to produce GA from glycerol containing methanol. This strain accumulated GA in its culture broth when 1-3 wt% glycerol was available as a carbon source. We observed improved cell growth and GA accumulation when 1 vol% methanol was added to the 3-5 wt% glycerol medium. The maximum concentration of GA was 12.8 g/L in medium containing 3 wt% glycerol plus 1 vol% methanol. In addition, the enantiomeric excess (ee) of the GA produced was revealed to be 44%, indicating that this strain converted glycerol to d-GA with a lower enantioselectivity than other acetic acid bacteria, which had 70-99% ee.

[1]  D. Kitamoto,et al.  Microbial resolution of DL-glyceric acid for L-glyceric acid production with newly isolated bacterial strains. , 2015, Journal of bioscience and bioengineering.

[2]  D. Kitamoto,et al.  Chemical mutagenesis of Gluconobacter frateurii to construct methanol-resistant mutants showing glyceric acid production from methanol-containing glycerol. , 2014, Journal of bioscience and bioengineering.

[3]  H. Hirakawa,et al.  Draft Genomic DNA Sequence of the Facultatively Methylotrophic Bacterium Acidomonas methanolica type strain MB58. , 2014, FEMS microbiology letters.

[4]  D. Kitamoto,et al.  Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol , 2013, AMB Express.

[5]  B. Quirino,et al.  Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste , 2012, Biotechnology for Biofuels.

[6]  R. Ashby,et al.  Efficient Utilization of Crude Glycerol as Fermentation Substrate in the Synthesis of Poly(3-hydroxybutyrate) Biopolymers , 2011 .

[7]  Carmela Napolitano,et al.  Highly stereoselective de novo synthesis of L-hexoses. , 2010, The Journal of organic chemistry.

[8]  M. Itagaki,et al.  Microbial Production of Glyceric Acid, an Organic Acid That Can Be Mass Produced from Glycerol , 2009, Applied and Environmental Microbiology.

[9]  M. Itagaki,et al.  Production of Glyceric Acid by Gluconobacter sp. NBRC3259 Using Raw Glycerol , 2009, Bioscience, biotechnology, and biochemistry.

[10]  D. Kitamoto,et al.  Biotransformation of glycerol to d-glyceric acid by Acetobacter tropicalis , 2009, Applied Microbiology and Biotechnology.

[11]  Yutaka Nakashimada,et al.  Hydrogen and ethanol production from glycerol-containing wastes discharged after biodiesel manufacturing process. , 2005, Journal of bioscience and bioengineering.

[12]  Seraphim Papanikolaou,et al.  Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture. , 2002, Bioresource technology.

[13]  K. Takahashi,et al.  Methanol and ethanol oxidase respiratory chains of the methylotrophic acetic acid bacterium, Acetobacter methanolicus. , 1992, Journal of biochemistry.