A novel nonenzymatic sensor based on CuO nanoneedle/graphene/carbon nanofiber modified electrode for probing glucose in saliva.

Here, we report on a novel nonenzymatic amperometric glucose sensor based on CuO nanoneedle/graphene/carbon nanofiber modified electrode. The results of the scanning electron microscopy indicate that electronic network was formed through their direct binding with the graphene/carbon nanofiber, which leads to larger active surface areas and faster electron transfer for the glucose sensor. High electrocatalytic activity toward the oxidation of glucose was observed with a rapid response (<2 s), a low detection limit (0.1 µM), a wide and useful linear range (1-5.3 mM) as well as good stability and repeatability. Moreover, the common interfering species, such as ascorbic acid, uric acid, dopamine and so forth did not cause obvious interference. The sensor can also be used for quantification of glucose concentration in real saliva samples. Therefore, this work has demonstrated a simple and effective sensing platform for nonenzymatic detection of glucose.

[1]  Z. Cheng,et al.  Capacitive detection of glucose using molecularly imprinted polymers. , 2001, Biosensors & bioelectronics.

[2]  Jing Luo,et al.  A novel non-enzymatic glucose sensor based on Cu nanoparticle modified graphene sheets electrode. , 2012, Analytica chimica acta.

[3]  Mukul Pradhan,et al.  Fabrication and Functionalization of CuO for Tuning Superhydrophobic Thin Film and Cotton Wool , 2011 .

[4]  Rajeev Kumar,et al.  Response speed of SnO2-based H2S gas sensors with CuO nanoparticles , 2004 .

[5]  X. G. Zhang,et al.  Hierarchically structured carbon-based composites: Design, synthesis and their application in electrochemical capacitors. , 2011, Nanoscale.

[6]  Yang Liu,et al.  Nonenzymatic glucose sensor based on renewable electrospun Ni nanoparticle-loaded carbon nanofiber paste electrode. , 2009, Biosensors & bioelectronics.

[7]  Masa-aki Morikawa,et al.  New colorimetric detection of glucose by means of electron-accepting indicators: ligand substitution of [Fe(acac)3-n(phen)n]n+ complexes triggered by electron transfer from glucose oxidase. , 2002, Chemistry.

[8]  Munetaka Oyama,et al.  Nonenzymatic amperometric sensing of glucose by using palladium nanoparticles supported on functional carbon nanotubes. , 2010, Biosensors & bioelectronics.

[9]  Liqiang Luo,et al.  A novel nitrite sensor based on graphene/polypyrrole/chitosan nanocomposite modified glassy carbon electrode. , 2011, The Analyst.

[10]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[11]  Huafeng Yang,et al.  Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene. , 2009, Analytical chemistry.

[12]  Hong Liu,et al.  Synthesis of CuO nanostructures and their application for nonenzymatic glucose sensing , 2010 .

[13]  Kang Wang,et al.  Highly Ordered Platinum‐Nanotubule Arrays for Amperometric Glucose Sensing , 2005 .

[14]  Joseph Wang.  Electrochemical glucose biosensors. , 2008, Chemical reviews.

[15]  Aicheng Chen,et al.  Nonenzymatic electrochemical glucose sensor based on nanoporous PtPb networks. , 2008, Analytical chemistry.

[16]  Jun Liu,et al.  Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. , 2009, Biosensors & bioelectronics.

[17]  Wen-Zhi Le,et al.  Preparation of nano-copper oxide modified glassy carbon electrode by a novel film plating/potential cycling method and its characterization , 2009 .

[18]  Huafeng Yang,et al.  Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing. , 2010, Biosensors & bioelectronics.

[19]  Guo-Li Shen,et al.  In situ synthesis of palladium nanoparticle-graphene nanohybrids and their application in nonenzymatic glucose biosensors. , 2011, Biosensors & bioelectronics.

[20]  Michael S Strano,et al.  In vivo fluorescence detection of glucose using a single-walled carbon nanotube optical sensor: design, fluorophore properties, advantages, and disadvantages. , 2005, Analytical chemistry.

[21]  F. Gao,et al.  Multifunctional carbon nanotubes for direct electrochemistry of glucose oxidase and glucose bioassay. , 2011, Biosensors & bioelectronics.

[22]  Hui Zhang,et al.  Nonenzymatic electrochemical detection of glucose based on palladium-single-walled carbon nanotube hybrid nanostructures. , 2009, Analytical chemistry.

[23]  Lun Wang,et al.  Enzyme-free amperometric sensing of glucose using Cu-CuO nanowire composites , 2010 .

[24]  A. Salimi,et al.  Non-enzymatic glucose detection free of ascorbic acid interference using nickel powder and nafion sol–gel dispersed renewable carbon ceramic electrode , 2005 .

[25]  Douglas R. Kauffman,et al.  Selective Electrocatalytic Activity of Ligand Stabilized Copper Oxide Nanoparticles , 2011 .

[26]  C. Huang,et al.  Localized surface plasmon resonance sensing detection of glucose in the serum samples of diabetes sufferers based on the redox reaction of chlorauric acid. , 2007, Talanta.

[27]  Yu Lei,et al.  CuO Nanospheres Based Nonenzymatic Glucose Sensor , 2008 .

[28]  Jonathan C. Claussen,et al.  Nanostructuring Platinum Nanoparticles on Multilayered Graphene Petal Nanosheets for Electrochemical Biosensing , 2012 .

[29]  Xin Li,et al.  Three-dimensional network films of electrospun copper oxide nanofibers for glucose determination. , 2009, Biosensors & bioelectronics.

[30]  Yaping Ding,et al.  Fabrication of Co3O4 nanoparticles-decorated graphene composite for determination of L-tryptophan. , 2012, The Analyst.

[31]  P. Pehrsson,et al.  Optical enzymatic detection of glucose based on hydrogen peroxide-sensitive HiPco carbon nanotubes , 2006 .

[32]  Impedance of porous electrodes , 1995 .

[33]  T. Ramachandran,et al.  Development of highly sensitive non-enzymatic sensor for the selective determination of glucose and fabrication of a working model , 2010 .

[34]  T. Cataldi,et al.  Highly-dispersed copper microparticles on the active gold substrate as an amperometric sensor for glucose , 1997 .

[35]  Andrew G. Glen,et al.  APPL , 2001 .

[36]  D. Xue,et al.  Room-Temperature Ferromagnetism of Flowerlike CuO Nanostructures , 2010 .

[37]  S. Stankovich,et al.  Synthesis of graphene-based nanosheets via chemical reduction of exfoliated graphite oxide , 2007 .