Growth and death in overagitated microcarrier cell cultures

The effects of hydrodynamic forces on cell growth are investigated for animal cells growing on microcarriers. A reduction in net growth was observed with high levels of agitation. DNA measurements indicated that the reduction in net growth was due entirely to cell death, from hydrodynamic forces. No inhibition or enhancement of cell replication appeared to occur with high levels of agitation.

[1]  H. A. Johnson.  Some problems associated with the histological study of cell proliferation kinetics. , 1961 .

[2]  D. L. Fry.  Acute Vascular Endothelial Changes Associated with Increased Blood Velocity Gradients , 1968, Circulation research.

[3]  J. Pontén,et al.  The division cycle and RNA-synthesis in diploid human cells at different passage levels in vitro. , 1966, Experimental cell research.

[4]  M. Stoker,et al.  Shaking 3T3 cells: further studies on diffusion boundary effects. , 1974, Cell.

[5]  M. Sharma,et al.  Alterations in bovine endothelial histidine decarboxylase activity following exposure to shearing stresses. , 1974, Experimental and molecular pathology.

[6]  E. Evans.  Bending elastic modulus of red blood cell membrane derived from buckling instability in micropipet aspiration tests. , 1983, Biophysical journal.

[7]  J. Bryant.  Methylcellulose effect on cell proliferation and glucose utilization in chemically defined medium in large stationary cultures , 1969, Biotechnology and bioengineering.

[8]  S. Shiigi,et al.  Sera and the in vitro induction of immune responses. I. Bacterial contamination and the generation of good fetal bovine sera. , 1975, Journal of immunology.

[9]  J. Tramper,et al.  Shear sensitivity of insect cells in suspension , 1986 .

[10]  L. Tolmach,et al.  Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. , 1963, Experimental cell research.

[11]  A J Sinskey,et al.  Effect of shear on the death of two strains of mammalian tissue cells , 1971, Biotechnology and bioengineering.

[12]  E. Papoutsakis,et al.  Physical mechanisms of cell damage in microcarrier cell culture bioreactors , 1988, Biotechnology and bioengineering.

[13]  T. Kenner,et al.  Arteriovenous difference of the blood density in the coronary circulation. , 1985, Journal of biomechanical engineering.

[14]  W. Thilly,et al.  Selection of mitotic Chinese hamster ovary cells from microcarriers. , 1980, Analytical biochemistry.

[15]  R M Nerem,et al.  Micropipette Aspiration of Cultured Bovine Aortic Endothelial Cells Exposed to Shear Stress , 1987, Arteriosclerosis.

[16]  R. Spier,et al.  The adhesion of animal cells to surfaces: The measurement of critical surface shear stress permitting attachment or causing detachment , 1985 .

[17]  N. Stathopoulos,et al.  Shear stress effects on human embryonic kidney cells in Vitro , 1985, Biotechnology and bioengineering.

[18]  M S Croughan,et al.  Hydrodynamic effects on animal cells grown in microcarrier cultures , 1987, Biotechnology and bioengineering.

[19]  Daniel I. C. Wang,et al.  Human interferon production with diploid fibroblast cells grown on microcarriers , 1979, Biotechnology and bioengineering.

[20]  J. Hamlin,et al.  Animal cell cycle. , 1978, Annual review of biochemistry.

[21]  D. I. Wang,et al.  Effects of microcarrier concentration in animal cell culture , 1988, Biotechnology and bioengineering.

[22]  M. G. P. STOKER,et al.  Role of Diffusion Boundary Layer in Contact Inhibition of Growth , 1973, Nature.

[23]  A J Sinskey,et al.  PRODUCTION OF CELL‐DERIVED PRODUCTS: VIRUS AND INTERFERON * , 1981, Annals of the New York Academy of Sciences.

[24]  T. W. James,et al.  Assay for nanogram quantities of DNA in cellular homogenates. , 1979, Analytical biochemistry.

[25]  R M Nerem,et al.  Influence of a laminar steady-state fluid-imposed wall shear stress on the binding, internalization, and degradation of low-density lipoproteins by cultured arterial endothelium. , 1987, Circulation.

[26]  L. Manson,et al.  Analysis of the Life-Cycle in Mammalian Cells , 1963, Nature.

[27]  N. G. Maroudas.  Appendix: Short-range diffusion gradients , 1974 .

[28]  J. M. Deforrest,et al.  Relationship between low intensity shear stress, aortic histamine formation, and aortic albumin uptake. , 1980, Experimental and molecular pathology.

[29]  J Meier,et al.  A mechanistic analysis of the inoculum requirement for the cultivation of mammalian cells on microcarriers , 1985, Biotechnology and bioengineering.

[30]  G. Dunn,et al.  New evidence that growth in 3T3 cell cultures is a diffusion-limited process , 1984, Nature.

[31]  R M Nerem,et al.  The elongation and orientation of cultured endothelial cells in response to shear stress. , 1985, Journal of biomechanical engineering.

[32]  L V McIntire,et al.  Flow effects on prostacyclin production by cultured human endothelial cells. , 1985, Science.

[33]  P. Greenfield,et al.  A technique for determining the shear sensitivity of mammalian cells in suspension culture , 1987 .

[34]  Robert K. Finn,et al.  A model system for evaluating shear in the design of stirred fermentors , 1966 .

[35]  T. M. Hollis,et al.  Effects of shearing stress on aortic histamine synthesis. , 1974, Experimental and molecular pathology.

[36]  N. Wright,et al.  An Introduction to Cell Population Kinetics , 1978 .