Various intensity of Proteus mirabilis-induced crystallization resulting from the changes in the mineral composition of urine.

Infectious urolithiasis is a result of recurrent and chronic urinary tract infections caused by urease-positive bacteria, especially Proteus mirabilis. The main role in the development of this kind of stones is played by bacterial factors such as urease and extracellular polysaccharides, but urinary tract environment also contributes to this process. We used an in vitro model to establish how the changes in the basic minerals concentrations affect the intensity of crystallization which occurs in urine. In each experiment crystallization was induced by an addition of P. mirabilis to artificial urine with a precisely defined chemical composition. Crystallization intensity was determined using the spectrophotometric microdilution method and the chemical composition of formed crystals was established by atomic absorption spectroscopy and colorimetric methods. Increasing the concentration of all crystals forming ions such as Mg(2+), Ca(2+) and phosphate strongly intensified the process of crystallization, whereas reducing the amount of these components below the proper physiological concentration did not affect its intensity. The inhibitory influence of citrate on calcium and magnesium phosphate crystallization and competitive actions of calcium and oxalate ions on struvite crystals formation were not confirmed. In the case of infectious stones the chemical composition of urine plays an important role, which creates a necessity to support the treatment by developing a model of proper diet.

[1]  A. Torzewska,et al.  Inhibition of crystallization caused by Proteus mirabilis during the development of infectious urolithiasis by various phenolic substances. , 2014, Microbiological research.

[2]  Tushar Kanti Sen,et al.  The influence of various physico-chemical process parameters on kinetics and growth mechanism of struvite crystallisation , 2014 .

[3]  W. Verstraete,et al.  Factors influencing urease driven struvite precipitation , 2013 .

[4]  V. Thongboonkerd,et al.  Extensive characterizations of bacteria isolated from catheterized urine and stone matrices in patients with nephrolithiasis. , 2012, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.

[5]  T. Knoll,et al.  Urolithiasis through the ages: data on more than 200,000 urinary stone analyses. , 2010, The Journal of urology.

[6]  T. Knoll.  Epidemiology, Pathogenesis, and Pathophysiology of Urolithiasis , 2010 .

[7]  J. Prywer,et al.  Bacterially Induced Struvite Growth from Synthetic Urine: Experimental and Theoretical Characterization of Crystal Morphology , 2009 .

[8]  A. Trinchieri,et al.  Infections and urolithiasis: current clinical evidence in prophylaxis and antibiotic therapy. , 2008, Archivio italiano di urologia, andrologia : organo ufficiale [di] Societa italiana di ecografia urologica e nefrologica.

[9]  Anthony J. Browning,et al.  The Role of Urinary Kidney Stone Inhibitors and Promoters in the Pathogenesis of Calcium Containing Renal Stones , 2007 .

[10]  Eugenia Valsami-Jones,et al.  Impact of calcium on struvite crystal size, shape and purity , 2005 .

[11]  R. McLean,et al.  Citrate and urease-induced crystallization in synthetic and human urine , 1993, Urological Research.

[12]  J. Greenman,et al.  Factors modulating the pH at which calcium and magnesium phosphates precipitate from human urine , 2005, Urological Research.

[13]  O. Yoshida,et al.  Effects of dietary calcium, magnesium and phosphorus on the formation of struvite stones in the urinary tract of rats , 2004, Urological Research.

[14]  J. Hugosson,et al.  How variations in the composition of urine influence urease-induced crystallization , 2004, Urological Research.

[15]  W. Achilles,et al.  The in-vivo effect of sodium-potassium citrate on the crystal growth rate of calcium oxalate and other parameters in human urine , 2004, Urological Research.

[16]  A. Torzewska,et al.  Crystallization of urine mineral components may depend on the chemical nature of Proteus endotoxin polysaccharides. , 2003, Journal of medical microbiology.

[17]  V. Braun,et al.  Urinary infection stones. , 2002, International journal of antimicrobial agents.

[18]  J. Lester,et al.  Conditions influencing the precipitation of magnesium ammonium phosphate. , 2001, Water research.

[19]  H. Klingler,et al.  Role of bacteria in the development of kidney stones , 2000, Current opinion in urology.

[20]  M. Iwanaga,et al.  Ascorbate conversion to oxalate in alkaline milieu and Proteus mirabilis culture. , 2000, Molecular urology.

[21]  C. Genestar,et al.  Inhibitory effect of pyrophosphate, citrate, magnesium and chondroitin sulphate in calcium oxalate urolithiasis. , 1989, British journal of urology.

[22]  S. Erickson,et al.  Infected Renal Lithiasis: Results of Long-Term Surgical and Medical Management , 1981 .

[23]  D. P. Griffith.  Struvite stones. , 1978, Kidney international.

[24]  D. Musher,et al.  Urease. The primary cause of infection-induced urinary stones. , 1976, Investigative urology.

[25]  K Hoeg,et al.  [Kidney stones]. , 1968, Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke.

[26]  B. Ames,et al.  The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. , 1960, The Journal of biological chemistry.

[27]  R. Flocks,et al.  Medical management of urolithiasis. , 1957, Postgraduate medicine.