Squeeze-chip: a finger-controlled microfluidic flow network device and its application to biochemical assays.

We designed and fabricated a novel microfluidic device that can be operated through simple finger squeezing. On-chip microfluidic flow control is enabled through an optimized network of check-valves and squeeze-pumps. The sophisticated flow system can be easily constructed by combining a few key elements. We implemented this device to perform quantitative biochemical assays with no requirement for precision instruments.

[1]  G. Whitesides,et al.  Complex Optical Surfaces Formed by Replica Molding Against Elastomeric Masters , 1996, Science.

[2]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[3]  Paul J. Choi,et al.  Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells , 2010, Science.

[4]  P Kabasakalian,et al.  Enzymatic blood glucose determination by colorimetry of N,N-diethylaniline-4-aminoantipyrine. , 1974, Clinical chemistry.

[5]  Chong H. Ahn,et al.  Institute of Physics Publishing Journal of Micromechanics and Microengineering a Review of Microvalves , 2022 .

[6]  Mark A Burns,et al.  Microfluidic pneumatic logic circuits and digital pneumatic microprocessors for integrated microfluidic systems. , 2009, Lab on a chip.

[7]  Roland Zengerle,et al.  Lab-on-a-Foil: microfluidics on thin and flexible films. , 2010, Lab on a chip.

[8]  Shuichi Takayama,et al.  Rapid Prototyping of Microstructures with Bell‐Shaped Cross‐Sections and Its Application to Deformation‐Based Microfluidic Valves , 2004 .

[9]  B. Mosadegh,et al.  Integrated Elastomeric Components for Autonomous Regulation of Sequential and Oscillatory Flow Switching in Microfluidic Devices , 2010, Nature physics.

[10]  Bruce K. Gale,et al.  Determining the optimal PDMS–PDMS bonding technique for microfluidic devices , 2008 .

[11]  Stephen R Quake,et al.  Whole-genome molecular haplotyping of single cells , 2011, Nature Biotechnology.

[12]  S. Quake,et al.  Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.

[13]  G. Whitesides The origins and the future of microfluidics , 2006, Nature.

[14]  J. Baek,et al.  Photopolymerized check valve and its integration into a pneumatic pumping system for biocompatible sample delivery. , 2006, Lab on a chip.

[15]  Sehwan Lee,et al.  Development of functional lab-on-a-chip on polymer for point-of-care testing of metabolic parameters. , 2008, Lab on a chip.

[16]  Daniel Malamud,et al.  An integrated, self-contained microfluidic cassette for isolation, amplification, and detection of nucleic acids , 2010, Biomedical microdevices.

[17]  Gang Wang,et al.  Microwave plasma treatment of polymer surface for irreversible sealing of microfluidic devices. , 2005, Lab on a chip.

[18]  Kristen L. Helton,et al.  Microfluidic Overview of Global Health Issues Microfluidic Diagnostic Technologies for Global Public Health , 2006 .

[19]  J B Findlay,et al.  Automated closed-vessel system for in vitro diagnostics based on polymerase chain reaction. , 1993, Clinical chemistry.

[20]  Axel Scherer,et al.  Polydimethylsiloxane based microfluidic diode , 2005 .

[21]  Daniel C Leslie,et al.  Frequency-specific flow control in microfluidic circuits with passive elastomeric features , 2009 .

[22]  Shuichi Takayama,et al.  High-density fabrication of normally closed microfluidic valves by patterned deactivation of oxidized polydimethylsiloxane. , 2011, Lab on a chip.

[23]  George M. Whitesides,et al.  Microfluidics Section: Design and Fabrication of Integrated Passive Valves and Pumps for Flexible Polymer 3-Dimensional Microfluidic Systems , 2002 .

[24]  F. Omenetto,et al.  Bio‐microfluidics: Biomaterials and Biomimetic Designs , 2010, Advanced materials.

[25]  D. Bodas,et al.  Hydrophilization and hydrophobic recovery of PDMS by oxygen plasma and chemical treatment—An SEM investigation , 2007 .

[26]  Xianbo Qiu,et al.  A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids. , 2009, Lab on a chip.

[27]  G. Berti,et al.  Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. , 1980, Clinical chemistry.

[28]  Daniel C Leslie,et al.  Nonlinear pressure-flow relationships for passive microfluidic valves. , 2009, Lab on a chip.

[29]  G. Whitesides,et al.  Patterned paper as a platform for inexpensive, low-volume, portable bioassays. , 2007, Angewandte Chemie.

[30]  Kiyotaka Sakai,et al.  Microfluidic Valves Comprising Nanolayered Thermoresponsive Polymer‐Grafted Capillaries , 2005 .

[31]  L. Gervais,et al.  Microfluidic Chips for Point‐of‐Care Immunodiagnostics , 2011, Advanced materials.