Silver-Decorated Polymeric Micelles Combined with Curcumin for Enhanced Antibacterial Activity.

Because of the mounting prevalence of complicated infections induced by multidrug-resistant bacteria, it is imperative to develop innovative and efficient antibacterial agents. In this work, we design a novel polymeric micelle for simultaneous decorating of silver nanoparticles and encapsulating of curcumin as a combination strategy to improve the antibacterial efficiency. In the constructed combination system, silver nanoparticles were decorated in the micellar shell because of the in situ reduction of silver ions, which were absorbed by the poly(aspartic acid) (PAsp) chains in the shell. Meanwhile, natural curcumin was encapsulated into the poly(ε-caprolactone) (PCL) core of the micelle through hydrophobic interaction. This strategy could prevent aggregation of silver nanoparticles and improve the water solubility of curcumin at the same time, which showed enhanced antibacterial activity toward Gram-negative P.aeruginosa and Gram-positive S.aureus compared with sliver-decorated micelle and curcumin-loaded micelle alone, due to the cooperative antibacterial effects of the silver nanoparticles and curcumin. Furthermore, the achieved combinational micelles had good biocompatibility and low hemolytic activity. Thus, our study provides a new pathway in the rational design of combination strategy for efficiently preventing the ubiquitous bacterial infections.

[1]  Omid Akhavan,et al.  Toxicity of graphene and graphene oxide nanowalls against bacteria. , 2010, ACS nano.

[2]  K. Zandi,et al.  A Review on Antibacterial, Antiviral, and Antifungal Activity of Curcumin , 2014, BioMed research international.

[3]  C Colson,et al.  Bacterial lipases. , 1994, FEMS microbiology reviews.

[4]  Amir S. Sharili,et al.  In Vitro Antibacterial Activity of Curcumin-Polymyxin B Combinations against Multidrug-Resistant Bacteria Associated with Traumatic Wound Infections. , 2016, Journal of natural products.

[5]  Ali Fakhimi,et al.  Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. , 2007, Nanomedicine : nanotechnology, biology, and medicine.

[6]  Menachem Elimelech,et al.  Single-walled carbon nanotubes exhibit strong antimicrobial activity. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[7]  W. Shao,et al.  Preparation, characterization, and antibacterial activity of silver nanoparticle-decorated graphene oxide nanocomposite. , 2015, ACS applied materials & interfaces.

[8]  Xuesi Chen,et al.  Co-delivery of doxorubicin and paclitaxel by PEG-polypeptide nanovehicle for the treatment of non-small cell lung cancer. , 2014, Biomaterials.

[9]  Linqi Shi,et al.  Effect of the Surface Charge of Artificial Chaperones on the Refolding of Thermally Denatured Lysozymes. , 2016, ACS applied materials & interfaces.

[10]  Hao Wang,et al.  Vancomycin-modified mesoporous silica nanoparticles for selective recognition and killing of pathogenic gram-positive bacteria over macrophage-like cells. , 2013, ACS applied materials & interfaces.

[11]  D. Patra,et al.  Fluorescence modulation of 1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione by silver nanoparticles and its possible analytical application. , 2012, Luminescence : the journal of biological and chemical luminescence.

[12]  J. Zink,et al.  Antimicrobial Activity of Silver Nanocrystals Encapsulated in Mesoporous Silica Nanoparticles , 2009 .

[13]  D. Pochan,et al.  Encapsulation of curcumin in self-assembling peptide hydrogels as injectable drug delivery vehicles. , 2011, Biomaterials.

[14]  Guohua Jiang,et al.  Deposition of Silver Nanoparticles on Multiwalled Carbon Nanotubes Grafted with Hyperbranched Poly(amidoamine) and Their Antimicrobial Effects , 2008 .

[15]  Bing Xu,et al.  Presenting Vancomycin on Nanoparticles to Enhance Antimicrobial Activities , 2003 .

[16]  Pranita D. Tamma,et al.  Combination Therapy for Treatment of Infections with Gram-Negative Bacteria , 2012, Clinical Microbiology Reviews.

[17]  Xingyu Jiang,et al.  Small molecule-capped gold nanoparticles as potent antibacterial agents that target Gram-negative bacteria. , 2010, Journal of the American Chemical Society.

[18]  Christian Melander,et al.  Combination approaches to combat multidrug-resistant bacteria. , 2013, Trends in biotechnology.

[19]  Abhishek Sahu,et al.  Fluorescence study of the curcumin-casein micelle complexation and its application as a drug nanocarrier to cancer cells. , 2008, Biomacromolecules.

[20]  Christopher T. Walsh,et al.  Antibiotics for Emerging Pathogens , 2009, Science.

[21]  Jun Wang,et al.  Lipase-sensitive polymeric triple-layered nanogel for "on-demand" drug delivery. , 2012, Journal of the American Chemical Society.

[22]  Fangyingkai Wang,et al.  Antibacterial Polymeric Nanostructures for Biomedical Applications , 2015 .

[23]  Dong Gun Lee,et al.  Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved. , 2012, Journal of medical microbiology.

[24]  J. Barrett,et al.  Antibiotics: where did we go wrong? , 2005, Drug discovery today.

[25]  Liangzhu Feng,et al.  Graphene oxide-silver nanocomposite as a highly effective antibacterial agent with species-specific mechanisms. , 2013, ACS applied materials & interfaces.

[26]  Ke Karlovu,et al.  The bactericidal effect of silver nanoparticles , 2010 .

[27]  D. Pochan,et al.  Design of an Injectable β‐Hairpin Peptide Hydrogel That Kills Methicillin‐Resistant Staphylococcus aureus , 2009 .

[28]  Young Jik Kwon,et al.  "Nanoantibiotics": a new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. , 2011, Journal of controlled release : official journal of the Controlled Release Society.

[29]  J. Jang,et al.  Antibacterial properties of novel poly(methyl methacrylate) nanofiber containing silver nanoparticles. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[30]  S. Levy,et al.  Antibacterial resistance worldwide: causes, challenges and responses , 2004, Nature Medicine.

[31]  Li Wei,et al.  Sharper and faster "nano darts" kill more bacteria: a study of antibacterial activity of individually dispersed pristine single-walled carbon nanotube. , 2009, ACS nano.

[32]  Rupesh Kumar Basniwal,et al.  Curcumin nanoparticles: preparation, characterization, and antimicrobial study. , 2011, Journal of agricultural and food chemistry.

[33]  Hong Chen,et al.  Synthesis of PS/Ag nanocomposite spheres with catalytic and antibacterial activities. , 2012, ACS applied materials & interfaces.

[34]  K. Jaeger,et al.  Bacterial lipolytic enzymes: classification and properties. , 1999, The Biochemical journal.

[35]  Karen L Wooley,et al.  Design of polymeric nanoparticles for biomedical delivery applications. , 2012, Chemical Society reviews.

[36]  D. Panda,et al.  Curcumin inhibits FtsZ assembly: an attractive mechanism for its antibacterial activity. , 2008, The Biochemical journal.

[37]  Haixiong Ge,et al.  Degradation behavior of poly(epsilon-caprolactone)-b-poly(ethylene glycol)-b-poly(epsilon-caprolactone) micelles in aqueous solution. , 2004, Biomacromolecules.

[38]  Matthias Epple,et al.  Silver as antibacterial agent: ion, nanoparticle, and metal. , 2013, Angewandte Chemie.

[39]  Weimin Fan,et al.  Self-assembled cationic peptide nanoparticles as an efficient antimicrobial agent. , 2009, Nature nanotechnology.

[40]  Tak W. Kee,et al.  Femtosecond transient absorption spectroscopy of copper(II)-curcumin complexes. , 2012, Physical chemistry chemical physics : PCCP.

[41]  M. Rai,et al.  Silver nanoparticles as a new generation of antimicrobials. , 2009, Biotechnology advances.

[42]  Peng Li,et al.  Cationic Peptidopolysaccharides Show Excellent Broad‐Spectrum Antimicrobial Activities and High Selectivity , 2012, Advanced materials.

[43]  Jianzhong Du,et al.  Silver-decorated biodegradable polymer vesicles with excellent antibacterial efficacy , 2014 .

[44]  Robert A Newman,et al.  Bioavailability of curcumin: problems and promises. , 2007, Molecular pharmaceutics.

[45]  I. Sondi,et al.  Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. , 2004, Journal of colloid and interface science.

[46]  Amanda C. Engler,et al.  Enhancement of Cationic Antimicrobial Materials via Cholesterol Incorporation , 2014, Advanced healthcare materials.

[47]  Xiaorui Wang,et al.  Enzyme-Responsive Polymeric Vesicles for Bacterial-Strain-Selective Delivery of Antimicrobial Agents. , 2016, Angewandte Chemie.

[48]  Linqi Shi,et al.  In vivo biodistribution of mixed shell micelles with tunable hydrophilic/hydrophobic surface. , 2013, Biomacromolecules.

[49]  P. Zhang,et al.  Functional Silver Nanoparticle as a Benign Antimicrobial Agent That Eradicates Antibiotic-Resistant Bacteria and Promotes Wound Healing. , 2016, ACS applied materials & interfaces.

[50]  S. S. Sinha,et al.  Mechanistic Study of the Synergistic Antibacterial Activity of Combined Silver Nanoparticles and Common Antibiotics. , 2016, Environmental science & technology.

[51]  Dae Hong Jeong,et al.  Antimicrobial effects of silver nanoparticles. , 2007, Nanomedicine : nanotechnology, biology, and medicine.

[52]  E. Gillies,et al.  Phosphonium-Functionalized Polymer Micelles with Intrinsic Antibacterial Activity. , 2017, Biomacromolecules.

[53]  Zhenkun Zhang,et al.  Surface-Adaptive, Antimicrobially Loaded, Micellar Nanocarriers with Enhanced Penetration and Killing Efficiency in Staphylococcal Biofilms. , 2016, ACS nano.

[54]  Karen L Wooley,et al.  Preparation and in vitro antimicrobial activity of silver-bearing degradable polymeric nanoparticles of polyphosphoester-block-poly(L-lactide). , 2015, ACS nano.

[55]  GREGORY N. TEW,et al.  De novo design of antimicrobial polymers, foldamers, and small molecules: from discovery to practical applications. , 2010, Accounts of chemical research.

[56]  Xingyu Jiang,et al.  Synergy of non-antibiotic drugs and pyrimidinethiol on gold nanoparticles against superbugs. , 2013, Journal of the American Chemical Society.

[57]  E. Hoek,et al.  A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment , 2010 .

[58]  George John,et al.  Silver-nanoparticle-embedded antimicrobial paints based on vegetable oil. , 2008, Nature materials.

[59]  Vincent M. Rotello,et al.  Fully Zwitterionic Nanoparticle Antimicrobial Agents through Tuning of Core Size and Ligand Structure. , 2016, ACS nano.

[60]  Ruchi Yadav,et al.  Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. , 2010, Nanomedicine : nanotechnology, biology, and medicine.

[61]  Katsuhiko Ariga,et al.  Antibacterial Effect of Silver-Incorporated Flake-Shell Nanoparticles under Dual-Modality. , 2016, ACS applied materials & interfaces.

[62]  Jianzhong Du,et al.  Water-dispersible and biodegradable polymer micelles with good antibacterial efficacy. , 2012, Chemical communications.