Response to a lethal dose of heat shock by a transient up‐regulation of clusterin expression followed by down‐regulation and apoptosis in prostate and bladder cancer cells

Clusterin is a ubiquitous, secretory glycoprotein with a wide array of functions. Recent studies have implicated that clusterin functioned as heat shock response proteins. The objective of the present study was to determine the fate of clusterin expression in cancer cells, which were subjected to a lethal dose of heat shock, in an attempt to shed light on mechanisms of action of clusterin.

[1]  R. Morimoto,et al.  Stress–inducible responses and heat shock proteins: New pharmacologic targets for cytoprotection , 1998, Nature Biotechnology.

[2]  J. Lakins,et al.  Clusterin in the male reproductive tract. , 1998, Journal of andrology.

[3]  A. Wyllie,et al.  Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis , 1984, The Journal of pathology.

[4]  S. Sayeed,et al.  Cytotoxic sensitivity to tumor necrosis factor‐α in PC3 and LNCaP prostatic cancer cells is regulated by extracellular levels of SGP‐2 (clusterin) , 1999, The Prostate.

[5]  S. North,et al.  Stress-induced transcription of the clusterin/apoJ gene. , 1997, The Biochemical journal.

[6]  F. Sharp,et al.  Heat-shock protein protection , 1999, Trends in Neurosciences.

[7]  C. Korch,et al.  TSU-Pr1 and JCA-1 cells are derivatives of T24 bladder carcinoma cells and are not of prostatic origin. , 2001, Cancer research.

[8]  I. Leav,et al.  Lack of association between enhanced TRPM-2/clusterin expression and increased apoptotic activity in sex-hormone-induced prostatic dysplasia of the Noble rat. , 1998, The American journal of pathology.

[9]  M. Griswold,et al.  Prevention of cell death induced by tumor necrosis factor alpha in LNCaP cells by overexpression of sulfated glycoprotein-2 (clusterin). , 1995, Cancer research.

[10]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[11]  R. Bailey,et al.  Clusterin in the male reproductive system: localization and possible function , 1999, Molecular and Cellular Endocrinology.

[12]  D. Holtzman,et al.  Clusterin contributes to caspase-3–independent brain injury following neonatal hypoxia-ischemia , 2001, Nature Medicine.

[13]  S. Lindquist,et al.  The heat-shock proteins. , 1988, Annual review of genetics.

[14]  M. Griswold,et al.  Purification and characterization of a sulfated glycoprotein secreted by Sertoli cells. , 1986, Biochemistry.

[15]  J. Tschopp,et al.  Quantitation of vitronectin and clusterin. Pitfalls and solutions in enzyme immunoassays for adhesive proteins. , 1993, Journal of immunological methods.

[16]  M. Santoro,et al.  Heat shock factors and the control of the stress response. , 2000, Biochemical pharmacology.

[17]  Y. Lazebnik,et al.  Caspases: enemies within. , 1998, Science.

[18]  J. Carver,et al.  Clusterin Has Chaperone-like Activity Similar to That of Small Heat Shock Proteins* , 1999, The Journal of Biological Chemistry.

[19]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[20]  L. French,et al.  Clusterin gene expression mediates resistance to apoptotic cell death induced by heat shock and oxidative stress. , 1999, The Journal of investigative dermatology.