Satellite DNA polymorphisms and AFLP correlate with Bos indicus-taurus hybridization.

We describe satellite DNA variation that detects hybridization of Bos indicus (zebu or indicine cattle) and Bos taurus (taurine cattle) in African cattle populations. On Southern blots hybridized to a satellite III probe, relative intensities of Hinfl fragments correlated with the taurine-zebu composition in hybrid animals as deduced from AFLP genotyping of the same animals and previous data on microsatellite allele frequencies. Similar results were obtained by PCR-RFLP analysis of a zebu-specific mutation in the repeat unit of satellite 1.711b. Analysis of individuals from 20 African cattle breeds indicate that the centromeric satellites of the sanga breeds are of the taurine type and that several East-African zebu breeds are hybrids between taurine and zebu. These satellite RFLP, or SFLP, markers provide a fast method to screen the genetic makeup of African cattle.

[1]  P. Ajmone-Marsan,et al.  AFLP markers for DNA fingerprinting in cattle. , 1997, Animal genetics.

[2]  J. Womack,et al.  An EcoRI polymorphism at the beta-nerve growth factor (NGFB) locus in cattle. , 1997, Animal genetics.

[3]  M. Shriver,et al.  Microsatellite DNA variation and the evolution, domestication and phylogeography of taurine and zebu cattle (Bos taurus and Bos indicus). , 1997, Genetics.

[4]  U. Landegren,et al.  Padlock probes reveal single-nucleotide differences, parent of origin and in situ distribution of centromeric sequences in human chromosomes 13 and 21 , 1997, Nature Genetics.

[5]  P. Lizardi,et al.  FISH with a twist , 1997, Nature Genetics.

[6]  B. Harrison,et al.  Classification of the southern African sanga and east African shorthorned zebu. , 1997, Animal genetics.

[7]  A. Darvasi,et al.  Genetic control of resistance to trypanosomiasis. , 1996, Veterinary immunology and immunopathology.

[8]  R. McDowell,et al.  Economic viability of crosses of Bos taurus and Bos indicus for dairying in warm climates. , 1996, Journal of dairy science.

[9]  D. Bradley,et al.  Mitochondrial diversity and the origins of African and European cattle. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[10]  John M. Hancock,et al.  Simple sequences and the expanding genome. , 1996, BioEssays : news and reviews in molecular, cellular and developmental biology.

[11]  D. Bradley,et al.  A polymorphism in randomly amplified DNA that differentiates the Y chromosomes of Bos indicus and Bos taurus. , 1995, Animal genetics.

[12]  P. Vos,et al.  AFLP: a new technique for DNA fingerprinting. , 1995, Nucleic acids research.

[13]  S. Kemp,et al.  Characterization of Zebu cattle breeds in Tanzania using random amplified polymorphic DNA markers. , 2009, Animal genetics.

[14]  S. Kemp,et al.  Randomly primed PCR amplification of pooled DNA reveals polymorphism in a ruminant repetitive DNA sequence which differentiates Bos indicus and B. taurus. , 2009, Animal genetics.

[15]  D. Bradley,et al.  Mitochondrial genetic variation in European, African and Indian cattle populations. , 2009, Animal genetics.

[16]  D. Bradley,et al.  Zebu-taurine variation in Y chromosomal DNA: a sensitive assay for genetic introgression in west African trypanotolerant cattle populations. , 2009, Animal genetics.

[17]  S. Kemp,et al.  Polymerase chain reaction analysis of mitochondrial DNA polymorphism in N'Dama and Zebu cattle. , 2009, Animal genetics.

[18]  W. Modi,et al.  Molecular organization and chromosomal localization of six highly repeated DNA families in the bovine genome , 1993 .

[19]  W. Morrison,et al.  Host susceptibility to African trypanosomiasis: trypanotolerance. , 1982, Advances in parasitology.

[20]  R. -. Streeck,et al.  Inserted sequences in bovine satellite DNA's. , 1981, Science.

[21]  Hans G. Zachau,et al.  Patchwork structure of a bovine satellite DNA , 1979, Cell.

[22]  D. M. Skinner.  Satellite DNA's , 1977 .

[23]  T. C. Cartwright,et al.  Sex chromosome polymorphism in domestic cattle. , 1968, The Journal of heredity.