Implementation of real‐time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry

Aims: The present study describes the implementation of real‐time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry.

[1]  M. Boissinot,et al.  Development of conventional and real-time PCR assays for the rapid detection of group B streptococci. , 2000, Clinical chemistry.

[2]  D. Gibson Rapid and definitive detection of Salmonella in foods by PCR. , 1998, Letters in applied microbiology.

[3]  C. Chiu,et al.  Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC, by an enrichment broth culture-multiplex PCR combination assay , 1996, Journal of clinical microbiology.

[4]  J. Luk,et al.  An enzyme-linked immunosorbent assay to detect PCR products of the rfbS gene from serogroup D salmonellae: a rapid screening prototype , 1997, Journal of clinical microbiology.

[5]  S. McVey,et al.  Detection of Salmonella serovars from clinical samples by enrichment broth cultivation-PCR procedure , 1994, Journal of clinical microbiology.

[6]  H. Tsen,et al.  Development and use of polymerase chain reaction for the specific detection of Salmonella Typhimurium in stool and food samples. , 1999, Journal of food protection.

[7]  H. Einsele,et al.  Quantification of Fungal DNA by Using Fluorescence Resonance Energy Transfer and the Light Cycler System , 2000, Journal of Clinical Microbiology.

[8]  N. Cohen,et al.  Comparison of polymerase chain reaction and microbiological culture for detection of salmonellae in equine feces and environmental samples. , 1996, American journal of veterinary research.

[9]  R. Colwell,et al.  Direct detection of Salmonella spp. in estuaries by using a DNA probe , 1990, Applied and environmental microbiology.

[10]  U J Balis,et al.  The LightCycler: a microvolume multisample fluorimeter with rapid temperature control. , 1997, BioTechniques.

[11]  G. Salvat,et al.  Identification by a multiplex PCR‐based assay of Salmonella Typhimurium and Salmonella Enteritidis strains from environmental swabs of poultry houses , 1999, Letters in applied microbiology.

[12]  M. Viljanen,et al.  Rapid Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi Sensu Stricto by LightCycler Fluorescence Melting Curve Analysis of a PCR Product of the recA Gene , 2000, Journal of Clinical Microbiology.

[13]  S A McEwen,et al.  Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. , 1992, Molecular and cellular probes.

[14]  A. Eyigor,et al.  Detection of Salmonellae in Chicken Feces by a Combination of Tetrathionate Broth Enrichment, Capillary PCR, and Capillary Gel Electrophoresis , 2001, Journal of Clinical Microbiology.

[15]  T. Hammack,et al.  Relative effectiveness of selenite cystine broth, tetrathionate broth, and Rappaport-Vassiliadis medium for the recovery of Salmonella spp. from foods with a low microbial load. , 1999, Journal of food protection.

[16]  C. Fricker The isolation of salmonellas and campylobacters. , 1987, The Journal of applied bacteriology.

[17]  J. E. Olsen,et al.  Research note: Detection of Salmonella in minced meat by the polymerase chain reaction method , 1995, Letters in applied microbiology.

[18]  Tennant,et al.  Rapid and definitive detection of Salmonella in foods by PCR , 1998, Letters in applied microbiology.

[19]  A. Eyigor,et al.  Prevalence of Salmonella serovars in chickens in Turkey. , 2001, Journal of food protection.