PHYTOCHEMICALS ANALYSIS AND IMMUNOMODULATORY ACTIVITY OF Saurauia vulcani Korth. LEAVES EXTRACTS TOWARDS RAW 264.7 CELL

Saurauia vulcani Korth. is an actinidiaceae family plant that is used as traditional medicine by the community. The immunomodulatory effect of Saurauia vulcani Korth. extracts were assessed using in vitro method on RAW 264.7 cells line by stratified extraction using n-hexane, ethyl acetate, and ethanol solvents. The viability of cells was determined with the MTT method. Determination of nitric oxide levels was assessed by the griess method and inhibition of gene expression TNF-α, IL-6, COX-2, IL-1β, and iNOS were determined by the RT-PCR method. Saurauia vulcani Korth. leaves (n-hexane, ethyl acetate, and ethanol) extracts at a concentration of 25 μg/mL were effected to reduce the level of nitric oxide in RAW 246.7 cells and was inhibited the expression of genes TNF-α, IL6, COX-2, IL-1β, and iNOS. The density value of each band formed by 1.21 ± 0.005; 1.15 ± 0.005; 0.88 ± 0.003 on TNF-α, 1.23 ± 0.003; 1.64 ± 0.005; 0.55 ± 0.003 on IL-6, 0.38 ± 0.003; 0.55 ± 0.003; 0.38 ± 0.003 on COX-2, 0.18 ± 0.003; 0.04 ± 0.003; 0.03 ± 0.003 on IL-1β, and 0.30 ± 0.003; 0.41 ± 0.003; 0.11 ± 0.005 on iNOS. Extracts could reduce NO production and inhibit gene expressions such as TNF-α, IL-6, COX-2, IL-1β, and iNOS. Keyword: Saurauia vulcani Korth, Extract, Immunomodulatory, RAW 246.7 Cell Line RASĀYAN J. Chem., Vol. 14, No.2, 202