The Lifespan-Extending Effects of Nymphaea hybrid Root Extract in the Nematode Caenorhabditis elegans

Nymphaea hybrid, a water lily from the Nymphaeaceae family, has been found to exhibit some in vivo beneficial effects. In the present study we investigated the lifespan-extending effects of Nymphaea hybrid root extract in the nematode Caenorhabditis elegans. We found that Nymphaea hybrid root extract significantly extended the lifespan of C.elegans and improved its locomotion during aging. Moreover, Nymphaea hybrid root extract increased the resistance of C.elegans to both heat stress and oxidative stress. We found that the ability of Nymphaea hybrid root extract to increase lifespan was independent of its antimicrobial effects and was probably associated with its effects on the reproduction of C.elegans. In addition, the lifespan-extending effects of Nymphaea hybrid root extract were found to be dependent on the insulin/IGF signaling pathway. We also found that total flavones of Nymphaea hybrid could increase survival of C.elegans in both normal and adverse conditions, indicating that total flavones comprise the major fractions with lifespan-extending effects. Therefore, Nymphaea hybrid root extract has lifespan-extending effects in C.elegans and could be developed as a functional food.

[1]  M. Labouesse.  [Caenorhabditis elegans]. , 2003, Medecine sciences : M/S.

[2]  C. Harley,et al.  Effect of vitamin E on lifespan and reproduction in Caenorhabditis elegans , 1988, Mechanisms of Ageing and Development.

[3]  D. Kim,et al.  Protocatechuic acid extends lifespan and increases stress resistance in Caenorhabditis elegans , 2013, Archives of Pharmacal Research.

[4]  S. Park,et al.  DDS, 4,4′-diaminodiphenylsulfone, extends organismic lifespan , 2010, Proceedings of the National Academy of Sciences.

[5]  S. Melov,et al.  Thermotolerance and extended life-span conferred by single-gene mutations and induced by thermal stress. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[6]  C. Murphy,et al.  C. Elegans as a Premier Longevity and Aging Model Insulin/igf-1 Signaling the Role of Insulin/igf-like Signaling in C. Elegans Longevity and Aging , 2022 .

[7]  J. Ordovás,et al.  Curcumin-supplemented diets increase superoxide dismutase activity and mean lifespan in Drosophila , 2012, AGE.

[8]  C. Peng,et al.  Black rice extract extends the lifespan of fruit flies. , 2012, Food & function.

[9]  N. Munakata.  [Genetics of Caenorhabditis elegans]. , 1989, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.

[10]  G. Lithgow,et al.  Using Caenorhabditis elegans as a Model for Aging and Age‐Related Diseases , 2006, Annals of the New York Academy of Sciences.

[11]  Kyung‐Jin Min,et al.  Korean mistletoe (Viscum album coloratum) extract extends the lifespan of nematodes and fruit flies , 2013, Biogerontology.

[12]  Siu Sylvia Lee,et al.  RNAi screens to identify components of gene networks that modulate aging in Caenorhabditis elegans. , 2010, Briefings in functional genomics.

[13]  O. Paredes-López,et al.  Berries: Improving Human Health and Healthy Aging, and Promoting Quality Life—A Review , 2010, Plant foods for human nutrition.

[14]  J. Collins,et al.  Pharmacology of delayed aging and extended lifespan of Caenorhabditis elegans , 2006, Experimental Gerontology.

[15]  Zebo Huang,et al.  The neuroprotective and lifespan-extension activities of Damnacanthus officinarum extracts in Caenorhabditis elegans. , 2012, Journal of ethnopharmacology.

[16]  A. Antebi,et al.  Regulation of longevity by the reproductive system , 2013, Experimental Gerontology.

[17]  Cynthia Kenyon,et al.  Signals from the reproductive system regulate the lifespan of C. elegans , 1999, Nature.

[18]  H. Tissenbaum.  Genetics, life span, health span, and the aging process in Caenorhabditis elegans. , 2012, The journals of gerontology. Series A, Biological sciences and medical sciences.

[19]  H. Aguilaniu,et al.  Reproduction, fat metabolism, and life span: what is the connection? , 2013, Cell metabolism.

[20]  Yukiko Sato,et al.  Pharmacological modulation of histone demethylase activity by a small molecule isolated from subcritical water extracts of Sasa senanensis leaves prolongs the lifespan of Drosophila melanogaster , 2012, BMC Complementary and Alternative Medicine.

[21]  G. Silverman,et al.  C. elegans in high-throughput drug discovery. , 2014, Advanced drug delivery reviews.

[22]  A. Antebi,et al.  Genetics of Aging in Caenorhabditis elegans , 2007, PLoS genetics.

[23]  A. Kler,et al.  Effects of Plant Extracts on the Reversal of Glucose-Induced Impairment of Stress-Resistance in Caenorhabditis elegans , 2014, Plant Foods for Human Nutrition.

[24]  E. Nollen,et al.  Genetic screens in Caenorhabditis elegans models for neurodegenerative diseases. , 2014, Biochimica et biophysica acta.

[25]  D. Ingram,et al.  Blueberry polyphenols increase lifespan and thermotolerance in Caenorhabditis elegans , 2006, Aging cell.

[26]  Ziheng Zhuang,et al.  Beneficial Effects of Wheat Gluten Hydrolysate to Extend Lifespan and Induce Stress Resistance in Nematode Caenorhabditis elegans , 2013, PloS one.

[27]  K. Evason,et al.  Anticonvulsant Medications Extend Worm Life-Span , 2005, Science.