Effect of Sucrose Concentration on Dental Biofilm Formed in situ and on Enamel Demineralization

The relationship between sucrose concentration and cariogenic potential was studied in situ. Adult volunteers wore intraoral palatal appliances containing human dental enamel blocks, which were extraorally submitted 8 times a day for 14 days, to the treatments: deionized distilled water and sucrose solutions from 1 to 40%. The biofilm formed was analyzed with respect to acidogenicity and biochemical composition; enamel demineralization was evaluated by microhardness. The results showed that 1% sucrose is less cariogenic than 5% or higher concentrations, although sucrose solution at 40% was still able to increase the concentration of insoluble polysaccharide in the biofilm formed. The findings suggest that the threshold of sucrose solution concentration for the formation of a cariogenic biofilm is 5%, which provided the same cariogenic potential as that observed for 10 and 20% sucrose solution.

[1]  L. Tenuta,et al.  Effect of starch on the cariogenic potential of sucrose , 2005, British Journal of Nutrition.

[2]  W. Bowen,et al.  Effect of Sucrose Containing Iron (II) on Dental Biofilm and Enamel Demineralization in situ , 2005, Caries Research.

[3]  A. F. Paes Leme,et al.  In situ Effect of Frequent Sucrose Exposure on Enamel Demineralization and on Plaque Composition after APF Application and F Dentifrice Use , 2004, Journal of dental research.

[4]  A. F. Paes Leme,et al.  Caries Progression and Inhibition in Human and Bovine Root Dentine in situ , 2003, Caries Research.

[5]  P D Marsh,et al.  Are dental diseases examples of ecological catastrophes? , 2003, Microbiology.

[6]  A. A. Del Bel Cury,et al.  Effect of Dentifrice Containing Fluoride and/or Baking Soda on Enamel Demineralization/ Remineralization: An in situ Study , 2001, Caries Research.

[7]  A. A. Del Bel Cury,et al.  Biochemical Composition and Cariogenicity of Dental Plaque Formed in the Presence of Sucrose or Glucose and Fructose , 2000, Caries Research.

[8]  R. Kent,et al.  The pH of Dental Plaque in its Relation to Early Enamel Caries and Dental Plaque Flora in Humans , 2000, Journal of dental research.

[9]  A. A. Del Bel Cury,et al.  In situ relationship between sucrose exposure and the composition of dental plaque. , 1997, Caries research.

[10]  J. van Houte,et al.  Role of Micro-organisms in Caries Etiology , 1994, Journal of dental research.

[11]  G. Dibdin,et al.  Physical and Biochemical Studies of Streptococcus mutans Sediments Suggest New Factors Linking the Cariogenicity of Plaque with its Extracellular Polysaccharide Content , 1988, Journal of dental research.

[12]  D J White,et al.  A longitudinal microhardness analysis of fluoride dentifrice effects on lesion progression in vitro. , 1987, Caries research.

[13]  J. van Houte,et al.  The Intra-oral Effect on Enamel Demineralization of Extracellular Matrix Material Synthesized from Sucrose by Streptococcus mutans , 1986, Journal of dental research.

[14]  A. Scheie,et al.  Role of sucrose in plaque formation. , 1985, Scandinavian journal of dental research.

[15]  J. van Houte,et al.  Enamel Demineralization by Mouthrinses Containing Different Concentrations of Sucrose , 1983, Journal of dental research.

[16]  T. Imfeld Identification of low caries risk dietary components. , 1983, Monographs in oral science.

[17]  J M ten Cate,et al.  Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. , 1983, Caries research.

[18]  W. Bowen,et al.  A method to assess cariogenic potential of foodstuffs. , 1980, Journal of the American Dental Association.

[19]  A. Hefti,et al.  Effect on caries incidence in rats of increasing dietary sucrose levels. , 1979, Caries research.