Ionic Permeability of the Inhibitory Postsynaptic Membrane of Lobster Muscle Fibers

Reversal potentials (E IPSP) of the inhibitory postsynaptic potential and the membrane resting potentials (EM) of lobster muscle fibers were determined with intracellular recording under a variety of ionic conditions. E IPSP is solely dependent on the electromotive force of anionic batteries; i.e., on the electrochemical gradient for a "mobile" fraction of intracellular Cl (Cli) which is considerably smaller than the total intracellular Cl. The active inhibitory membrane is more permeable to certain "foreign" anions in the order NO3 > SCN > Br > Cl. The membrane is impermeable to BrOs, isethionate, and methylsulfate, but is slightly permeable to acetate and propionate. The level of Cli appears to be determined in part by some active (pump?) process and most of the anions studied appear to interfere with the steady-state level of Cli.

[1]  K. Krnjević,et al.  Anionic permeability of cortical neurones during inhibition. , 1968, The Journal of physiology.

[2]  H. Gainer,et al.  The distribution of inorganic ions in lobster muscle. , 1968, Biochimica et biophysica acta.

[3]  H. Gainer,et al.  Permeability of Alkali Metal Cations in Lobster Muscle , 1968, The Journal of general physiology.

[4]  H. Grundfest.  Some comparative biological aspects of membrane permeability control. , 1967, Federation proceedings.

[5]  A. Takeuchi,et al.  Anion permeability of the inhibitory post‐synaptic membrane of the crayfish neuromuscular junction , 1967, The Journal of physiology.

[6]  H. Gainer,et al.  The augmentation of postsynaptic potentials in crustacean muscle fibers by cesium. A presynaptic mechanism , 1967 .

[7]  H. Grundfest,et al.  Crayfish Muscle Fiber: Ionic Requirements for Depolarizing Synaptic Electrogenesis , 1967, Science.

[8]  A. R. Freeman,et al.  Osmometrically Determined Characteristics of the Cell Membrane of Squid and Lobster Giant Axons , 1966, The Journal of general physiology.

[9]  A. R. Freeman,et al.  The Membrane Components of Crustacean Neuromuscular Systems. , 1966, The Journal of general physiology.

[10]  A. Takeuchi,et al.  On the permeability of the presynaptic terminal of the crayfish neuromuscular junction during synaptic inhibition and the action of γ‐aminobutyric acid , 1966, The Journal of physiology.

[11]  H. Grundfest,et al.  Water Transfer and Cell Structure in Isolated Crayfish Muscle Fibers , 1964, The Journal of general physiology.

[12]  G. A. Kerkut,et al.  THE EFFECT OF ANION INJECTION AND CHANGES IN THE EXTERNAL POTASSIUM AND CHLORIDE CONCENTRATION ON THE REVERSAL POTENTIALS OF THE IPSP AND ACETYLCHOLINE. , 1964, Comparative biochemistry and physiology.

[13]  M. Ito,et al.  Further study on anion permeability of inhibitory post‐synaptic membrane of cat motoneurones , 1962, The Journal of physiology.

[14]  M. Ito,et al.  Anion permeability of the synaptic and non‐synaptic motoneurone membrane , 1961, The Journal of physiology.

[15]  R. Werman,et al.  Graded and All-or-None Electrogenesis in Arthropod Muscle , 1961, The Journal of general physiology.

[16]  A. Hodgkin,et al.  The influence of potassium and chloride ions on the membrane potential of single muscle fibres , 1959, The Journal of physiology.

[17]  H. Grundfest,et al.  THE ELECTROPHYSIOLOGY AND PHARMACOLOGY OF LOBSTER NEUROMUSCULAR SYNAPSES , 1959, The Journal of general physiology.

[18]  E. J. Harris.  Anion interaction in frog muscle , 1958, The Journal of physiology.

[19]  J. C. Dalton.  EFFECTS OF EXTERNAL IONS ON MEMBRANE POTENTIALS OF A LOBSTER GIANT AXON , 1958, The Journal of general physiology.

[20]  A. Hodgkin,et al.  THE IONIC BASIS OF ELECTRICAL ACTIVITY IN NERVE AND MUSCLE , 1951 .

[21]  A. Hodgkin,et al.  The effect of sodium ions on the electrical activity of the giant axon of the squid , 1949, The Journal of physiology.

[22]  D. E. Goldman.  POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANES , 1943, The Journal of general physiology.

[23]  E. Conway,et al.  Potassium accumulation in muscle and associated changes 1 , 1941 .

[24]  H. Grundfest,et al.  Comparative electrobiology of excitable membranes. , 1966, Advances in comparative physiology and biochemistry.

[25]  E. Ernst.  Biophysics of the striated muscle , 1963 .

[26]  H. Grundfest.  Ionic Transport across Neural and Non-Neural Membranes , 1962 .

[27]  E J Conway,et al.  Potassium accumulation in muscle and associated changes. , 1941, The Journal of physiology.