论文信息 - Interferometric label-free biomolecular detection system

Interferometric label-free biomolecular detection system

This work presents a simple evanescent wave sensing system based on an interferometric approach, suitable to meet the requirements of label-free sensor systems for detecting biomolecular interactions. It represents a basic concept towards label-free detection systems in various applications. The basic objectives of transducers for evanescent wave sensing are discussed. An optical detection system based on a interferometric approach using Young's double slit configuration is discussed, set-up and characterized. With refractometric measurements of various sucrose dilutions, the performance of the pure optical set-up is evaluated. A mean resolution of the effective refractive index of without averaging was obtained and a reproducibility below σr(neff) = 0.1 × 10−6 was achieved. Furthermore basic experiments were carried out, for proofing the concept's suitability as a highly sensitive biosensor by detecting the hybridization of 21-mer DNA with an immobilized counterpart on the surface.

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