In vitro temperature sensitivity of DNA, RNA, and protein syntheses throughout puberty in human testis.

To evaluate the optimal temperature for DNA, RNA, and protein syntheses in the prepubertal, pubertal, and postpubertal human testis, the levels of incorporation of 3H-thymidine, 14C-uridine, and 14C-leucine into cultured testicular tissue were studied at 28 degrees C, 31 degrees C, 34 degrees C, 37 degrees C, 40 degrees C, and 43 degrees C. The maximum level of 3H-thymidine incorporation in prepubertal testes occurred at 37 degrees C, whereas the maximum incorporation in the pubertal and postpubertal testes occurred at 31 degrees C. Incorporations of 14C-uridine and 14C-leucine in the three groups were temperature dependent (28 degrees C to 37 degrees C). DNA synthesis by germ cells in the pubertal and postpubertal testes was maximum at 31 degrees C and was impaired at body temperature (37 degrees C), whereas in the prepubertal testis it was temperature dependent with a maximum at 37 degrees C. RNA and protein synthesis in the three groups was temperature dependent at 28 degrees C to 37 degrees C, was depressed at 40 degrees C, and remarkably depressed at 43 degrees C.

[1]  S. Ying.  Inhibins, activins, and follistatins: gonadal proteins modulating the secretion of follicle-stimulating hormone. , 1988, Endocrine reviews.

[2]  K. Matsumoto,et al.  Influence of temperature on the function of Sertoli and Leydig cells of human testes. , 1987, Fertility and sterility.

[3]  Y. Nishimune,et al.  Etinoic acid (vitamin A acid) induces spermatogenesis in adult mouse cryptorchid testes in vitro. , 1982, Biochemical and biophysical research communications.

[4]  K. Matsumoto,et al.  Pituitary and gonadal function in prepubertal and pubertal boys with hypospadias. , 1981, Acta endocrinologica.

[5]  Y. Nishimune,et al.  Temperature-sensitivity of mouse testicular DNA synthesis in vitro. , 1972, Experimental cell research.

[6]  W. Perloff,et al.  MAMMALIAN TESTES IN ORGAN CULTURE. , 1964, Experimental cell research.

[7]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[8]  W. C. Schneider.  Phosphorus compounds in animal tissues; a comparison of methods for the estimation of nucleic acids. , 1946, The Journal of biological chemistry.

[9]  M Namiki,et al.  Optimal temperature for synthesis of DNA, RNA, and protein by human testis in vitro. , 1988, Archives of andrology.

[10]  K. Matsumoto,et al.  Temperature sensitivity of human spermatogonia and spermatocytes in vitro. , 1987, Archives of andrology.

[11]  L. Dubin,et al.  Other factors affecting male fertility. , 1977 .

[12]  S. Johnsen.  Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. , 1970, Hormones.

[13]  J. Davis,et al.  Species variation in the effect of temperature on the incorporation of L-lysine-U-C14 into protein of testis slices. , 1966, Comparative biochemistry and physiology.