Optical tweezers study life under tension.

Optical tweezers have become one of the primary weapons in the arsenal of biophysicists, and have revolutionized the new field of single-molecule biophysics. Today's techniques allow high-resolution experiments on biological macromolecules that were mere pipe dreams only a decade ago.

[1]  Michelle D. Wang,et al.  Stretching DNA with optical tweezers. , 1997, Biophysical journal.

[2]  D. Grier.  A revolution in optical manipulation , 2003, Nature.

[3]  S. Block,et al.  Single-molecule studies of RNA polymerase: one singular sensation, every little step it takes. , 2011, Molecular cell.

[4]  Steven M. Block,et al.  Kinesin Moves by an Asymmetric Hand-OverHand Mechanism , 2003 .

[5]  Steven M. Block,et al.  Direct observation of the binding state of the kinesin head to the microtubule , 2009, Nature.

[6]  D. Herschlag,et al.  Direct Measurement of the Full, Sequence-Dependent Folding Landscape of a Nucleic Acid , 2006, Science.

[7]  S. Block,et al.  Versatile optical traps with feedback control. , 1998, Methods in enzymology.

[8]  Steven M Block,et al.  Forward and reverse motion of single RecBCD molecules on DNA. , 2004, Biophysical journal.

[9]  I. Tinoco,et al.  RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP , 2006, Nature.

[10]  Joshua W. Shaevitz,et al.  Probing the kinesin reaction cycle with a 2D optical force clamp , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Liedewij Laan,et al.  Assembly dynamics of microtubules at molecular resolution , 2006, Nature.

[12]  Ignacio Tinoco,et al.  Following translation by single ribosomes one codon at a time , 2008, Nature.

[13]  K. Schulten,et al.  Fluorescence-Force Spectroscopy Maps Two-Dimensional Reaction Landscape of the Holliday Junction , 2007, Science.

[14]  E. Katayama,et al.  Inner-arm dynein c of Chlamydomonas flagella is a single-headed processive motor , 1999, Nature.

[15]  J. Liphardt,et al.  Reversible Unfolding of Single RNA Molecules by Mechanical Force , 2001, Science.

[16]  Michelle D. Wang,et al.  Optical torque wrench: angular trapping, rotation, and torque detection of quartz microparticles. , 2004, Physical review letters.

[17]  Matthew J Lang,et al.  Interlaced optical force-fluorescence measurements for single molecule biophysics. , 2006, Biophysical journal.

[18]  K. Svoboda,et al.  Biological applications of optical forces. , 1994, Annual review of biophysics and biomolecular structure.

[19]  Polly M Fordyce,et al.  Simultaneous, coincident optical trapping and single-molecule fluorescence , 2004, Nature Methods.

[20]  Carlos Bustamante,et al.  Supplemental data for : The Bacteriophage ø 29 Portal Motor can Package DNA Against a Large Internal Force , 2001 .

[21]  Carlos Bustamante,et al.  Differential detection of dual traps improves the spatial resolution of optical tweezers. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Gijs J. L. Wuite,et al.  Counting RAD51 proteins disassembling from nucleoprotein filaments under tension , 2008, Nature.

[23]  S. Smith,et al.  Folding-unfolding transitions in single titin molecules characterized with laser tweezers. , 1997, Science.

[24]  W. Greenleaf,et al.  Direct observation of base-pair stepping by RNA polymerase , 2005, Nature.

[25]  Elizabeth A. Shank,et al.  The folding cooperativity of a protein is controlled by its chain topology , 2010, Nature.

[26]  Christoph F. Schmidt,et al.  Direct observation of kinesin stepping by optical trapping interferometry , 1993, Nature.

[27]  Carlos Bustamante,et al.  Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner , 2007, Nature.

[28]  S. Chu,et al.  Observation of a single-beam gradient force optical trap for dielectric particles. , 1986, Optics letters.

[29]  Kirsten L. Frieda,et al.  Direct Observation of Hierarchical Folding in Single Riboswitch Aptamers , 2008, Science.

[30]  Steven M Block,et al.  Passive all-optical force clamp for high-resolution laser trapping. , 2005, Physical review letters.

[31]  Ashley R. Carter,et al.  Stabilization of an Optical Microscope to 0.1 Nm in Three Dimensions , 2022 .

[32]  J. Spudich,et al.  Single myosin molecule mechanics: piconewton forces and nanometre steps , 1994, Nature.

[33]  T. Ha,et al.  Ultrahigh-resolution optical trap with single-fluorophore sensitivity , 2011, Nature Methods.