Infectious Prions in the Saliva and Blood of Deer with Chronic Wasting Disease

A critical concern in the transmission of prion diseases, including chronic wasting disease (CWD) of cervids, is the potential presence of prions in body fluids. To address this issue directly, we exposed cohorts of CWD-naïve deer to saliva, blood, or urine and feces from CWD-positive deer. We found infectious prions capable of transmitting CWD in saliva (by the oral route) and in blood (by transfusion). The results help to explain the facile transmission of CWD among cervids and prompt caution concerning contact with body fluids in prion infections.

[1]  J Mackenzie,et al.  Possible transmission of variant Creutzfeldt-Jakob disease by blood transfusion , 2004, The Lancet.

[2]  T. Besser,et al.  Polymorphisms in the prion precursor functional gene but not the pseudogene are associated with susceptibility to chronic wasting disease in white-tailed deer. , 2004, The Journal of general virology.

[3]  N. Thompson Hobbs,et al.  Environmental Sources of Prion Transmission in Mule Deer , 2004, Emerging infectious diseases.

[4]  Manjit,et al.  Neurology , 1912, NeuroImage.

[5]  E. Williams,et al.  SPONGIFORM ENCEPHALOPATHY OF ROCKY MOUNTAIN ELK1 , 1982, Journal of wildlife diseases.

[6]  F. Cohen,et al.  Identification of Two Prion Protein Regions That Modify Scrapie Incubation Time , 2001, Journal of Virology.

[7]  D. Haig,et al.  Presence of the transmissible agent of scrapie in the serum of affected mice and rats , 1967, Veterinary Record.

[8]  A. Espenes,et al.  Transportation of prion protein across the intestinal mucosa of scrapie‐susceptible and scrapie‐resistant sheep , 2006, The Journal of pathology.

[9]  J. Ashby.  References and Notes , 1999 .

[10]  M. Head,et al.  Preclinical vCJD after blood transfusion in a PRNP codon 129 heterozygous patient , 2004, The Lancet.

[11]  N. Hunter,et al.  Transmission of prion diseases by blood transfusion. , 2002, The Journal of general virology.

[12]  M. Groschup,et al.  Degradation of scrapie associated prion protein (PrPSc) by the gastrointestinal microbiota of cattle. , 2006, Veterinary research.

[13]  R. Quatrano.  Genomics , 1998, Plant Cell.

[14]  M. Miller,et al.  Chronic wasting disease of cervids. , 2004, Current topics in microbiology and immunology.

[15]  E. Williams,et al.  CHRONIC WASTING DISEASE OF CAPTIVE MULE DEER: A SPONGIFORM ENCEPHALOPATHY1 , 1980, Journal of wildlife diseases.

[16]  A. Aguzzi,et al.  Extraneural pathologic prion protein in sporadic Creutzfeldt-Jakob disease. , 2003, The New England journal of medicine.

[17]  J. Winn,et al.  Brain , 1878, The Lancet.

[18]  C. Eklund,et al.  Course of experimental scrapie virus infection in the goat. , 1974, The Journal of infectious diseases.

[19]  C. Eklund,et al.  Pathogenesis of scrapie virus infection in the mouse. , 1967, The Journal of infectious diseases.

[20]  P. Barretti,et al.  Transfusion , 1889, The Hospital.

[21]  R. Bessen,et al.  Prion Infection of Oral and Nasal Mucosa , 2006, Journal of Virology.

[22]  M. Miller,et al.  SPONGIFORM ENCEPHALOPATHY IN FREE-RANGING MULE DEER (ODOCOILEUS HEMIONUS), WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS) AND ROCKY MOUNTAIN ELK (CERVUS ELAPHUS NELSONI) IN NORTHCENTRAL COLORADO , 1997, Journal of wildlife diseases.

[23]  L. McShane,et al.  Similar levels of infectivity in the blood of mice infected with human‐derived vCJD and GSS strains of transmissible spongiform encephalopathy , 2003, Transfusion.

[24]  E. Williams,et al.  Transmission of Prions from Mule Deer and Elk with Chronic Wasting Disease to Transgenic Mice Expressing Cervid PrP , 2004, Journal of Virology.

[25]  R. Kennedy,et al.  Natural infection of Suffolk sheep with scrapie virus. , 1982, The Journal of infectious diseases.

[26]  N. Hunter,et al.  Transmission of BSE by blood transfusion in sheep , 2000, The Lancet.