Development of Complete Plantlets from Moderately Vigorous Shoots of Stock Plants of Asparagus In Vitro1

One-bud segments from moderately vigorous shoots of aseptically grown Asparagus officinalis L. stock plants were cultured on modified Murashige and Skoog’s medium (MMS) containing 0.1 ppm of α-naphthaleneacetic acid (NAA) and 6-furfurylamino purine (kinetin). Only 35% of the cultures developed into rooted plantlets. A high percentage of nonrooted plantlets were induced to root by reculturing on fresh MMS medium containing 0.1 ppm NAA. More plantlets rooted if they were older than 4 weeks when recultured on the fresh medium.