An examination of an iterative method for the solution of the phase problem in optics and electron optics: I. Test calculations

An evaluation is made of an iterative method for determining the amplitude and phase from the image intensity recorded in optical systems. The method, which requires two images recorded at different lens defocus values, is tested with simulated data subject to error arising from the photographic recording of the image. In the case of error-free data, the solution for the phase distribution appears to be indeterminate to within a constant. The results for photographic noise levels of up to 20% of the maximum image intensity reflect the small effect of error on the calculated phase distribution. The calculation of phase distributions for both symmetric and asymmetric amplitude-phase distributions shows that the use of two images, taken at defocus values differing by about 100 nm in electron optics and about 1 mm in optics (depending on the numerical aperture of the objective lens), may be used to determine the complex object wave-function in both dark-field and bright-field optics.

[1]  E. Wolf Is a Complete Determination of the Energy Spectrum of Light Possible from Measurements of the Degree of Coherence , 1962 .

[2]  Les Relations de Dispersion dans la Théorie des Images Optiques , 1963 .

[3]  A. Walther The Question of Phase Retrieval in Optics , 1963 .

[4]  A. Marathay,et al.  Analyticity and phase retrieval , 1963 .

[5]  Edward L. O’Neill,et al.  The Question of Phase in Image Formation , 1963 .

[6]  E. Wolf,et al.  Some Theorems on the Unimodular Complex Degree of Optical Coherence , 1966 .

[7]  H. Nussenzveig,et al.  Phase Problem in Coherence Theory , 1967 .

[8]  Dorian Kermisch,et al.  Image Reconstruction from Phase Information Only , 1970 .

[9]  W. Hoppe,et al.  Principles of electron structure research at atomic resolution using conventional electron microscopes for the measurement of amplitudes and phases , 1970 .

[10]  A. Klug,et al.  Measurement and compensation of defocusing and aberrations by Fourier processing of electron micrographs , 1971 .

[11]  W. Hoppe Use of zone correction plates and other techniques for structure determination of aperiodic objects at atomic resolution using a conventional electron microscope , 1971 .

[12]  R. Gerchberg A practical algorithm for the determination of phase from image and diffraction plane pictures , 1972 .

[13]  R. Gerchberg,et al.  Holography without Fringes in the Electron Microscope , 1972, Nature.

[14]  J Frank,et al.  A study of heavy-light atom discrimination in bright-field electron microscopy using the computer. , 1972, Biophysical journal.

[15]  D. L. Misell Comment onA method for the solution of the phase problem in electron microscopy , 1973 .

[16]  D L Misell On the electron microscopy of biological specimens , 1973 .