[Objective]The study aimed to research the effect of plant hormone ratio on tissue culture of Petunia hybrida.[Method] Leaves of Petunia hybrida was used for explant to study effect of plant hormone ratio on callus,bud induction and root formation.[Result] The results showed that MS+6-BA 2.0 mg/L+ NAA 0.2 mg/L was suited for callus and bud induction;1/2 MS+NAA 0.1 mg/L or 1/2 MS+IBA 0.1 mg/L were suited for root formation.[Conclusion]It provided basic data for the establishment of rapid propogation,indigenous resources conservation and genetic transformation of Petunia hybrida.