Inherent cellular radiosensitivity as a basic concept for human tumor radiotherapy.

Abstract A statistical analysis has been performed on a set of 59 published survival curves of human cell lines. Six fibroblast cell lines derived from patients free of genetic disorders and 36 tumor cell lines were used in this study. Using the linear quadratic (L-Q) model, which provides an overall adequate fitting, especially in the low dose range, we show that great variations in radiosensitivity exist among cell lines. In the low dose range (≅2 Gy), these variations cannot be explained on the mere basis of technical factors. Cell type is described as an intrinsic radiosensitivity factor, as variations of mean radiosensitivity among cell types are statistically significant at low doses. A correlation is found between the 95% tumor control dose and the mean surviving fraction at 2 Gy for a given cell type. Higher radiosensitivity is accompanied by lower tumor control dose (TCD 95 % ). This correlation suggests that the moderate radiocurability of certain tumors can be partially explained by the intrinsic radiosensitivity of relevant tumor cells and in particular by a high surviving fraction at 2 Gy.

[1]  S. Luria,et al.  Ultraviolet Irradiation of Bacteriophage During Intracellular Growth , 1947, Journal of bacteriology.

[2]  R. Weichselbaum,et al.  In vitro cellular radiosensitivity of human malignant tumors. , 1976, European journal of cancer.

[3]  Albrecht M. Kellerer,et al.  THEORY OF DUAL RADIATION ACTION. , 1972 .

[4]  P. Deschavanne,et al.  In vitro radiosensitivity of six human cell lines: a comparative study with different statistical models. , 1980, Radiation research.

[5]  G J Neary,et al.  Chromosome aberrations and the theory of RBE. 1. General considerations. , 1965, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[6]  R. Weichselbaum,et al.  Inherent cellular radiosensitivity of human tumors of varying clinical curability. , 1976, AJR. American journal of roentgenology.

[7]  J. P. Keene,et al.  Absence of a fractionation effect in irradiated HeLa cells. , 1973, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[8]  N. J. McNally,et al.  The effect of radiation on tumour growth delay, cell survival and cure of the animal using a single tumour system. , 1977, The British journal of radiology.

[9]  C. Smith,et al.  Oxygen equilibration problems in the determination of K for HeLa S 3 (OXF). , 1972, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[10]  R. Berry,et al.  Perturbations in x-ray dose response in vitro with time after plating: a pitfall in the comparison of results obtained by different laboratories using asynchronous cell systems. , 1966, Experimental cell research.

[11]  E. Malaise,et al.  Radiosensitivity and growth parameters in vitro of three human melanoma cell strains. , 1978, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[12]  J. Bedford,et al.  Dose-rate effects in plateau-phase cultures of S3 HeLa and V79 cells. , 1979, Radiation research.

[13]  R. Humphrey,et al.  The response of a human malignant melanoma cell line to high LET radiation. , 1975, Radiology.

[14]  R. Lockart,et al.  Radiation response of mammalian cells grown in culture. II. Survival and recovery characteristics of several subcultures of HeLa S3 cells after x-irradiation. , 1961, Journal of the National Cancer Institute.

[15]  J. Fowler,et al.  Radiation Effects on Normal Tissues , 1977 .

[16]  R. Weichselbaum,et al.  X-ray sensitivity of fifty-three human diploid fibroblast cell strains from patients with characterized genetic disorders. , 1980, Cancer research.

[17]  E. Pettersen,et al.  Recovery from x-ray induced damage in human cells grown in culture. , 1977, Neoplasma.

[18]  E. Hall,et al.  DOSE RATE: ITS EFFECT ON THE SURVIVAL OF HELA CELLS IRRADIATED WITH GAMMA RAYS. , 1964, Radiation research.

[19]  F. James Rohlf,et al.  Biometry: The Principles and Practice of Statistics in Biological Research , 1969 .

[20]  A. Szechter,et al.  Continuous and fractionated irradiation of mammalian cells in culture--I. The effect of growth rate. , 1978, International journal of radiation oncology, biology, physics.

[21]  L. Gerweck,et al.  Radiation sensitivity of cultured human glioblastoma cells. , 1977, Radiology.

[22]  R. Katz.  Track Theory and Radiation Quality , 1971 .

[23]  S. B. Field.  Early and late normal tissue damage after fast neutrons. , 1977, International journal of radiation oncology, biology, physics.

[24]  P. Rubin,et al.  Range of prescribed tumor lethal doses (PTLD) in the treatment of different human tumors , 1974 .

[25]  V. Courtenay,et al.  In vitro radiation response of cells from four human tumors propagated in immune-suppressed mice. , 1978, Cancer research.

[26]  M. Lavin,et al.  Dependence on cloning method of survival of human melanoma cells after ultraviolet and ionizing radiation. , 1978, Cancer research.

[27]  J. Savage,et al.  Chromosome aberrations and the theory of RBE. II. Evidence from track-segment experiments with protons and alpha particles. , 1966, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[28]  P. Bryant,et al.  Changes in sensitivity of HeLa cells after split doses of x-rays. , 1979, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[29]  D. Goodhead,et al.  Inactivation and mutation of cultured mammalian cells by aluminium characteristic ultrasoft X-rays. II. Dose-responses of Chinese hamster and human diploid cells to aluminium X-rays and radiations of different LET. , 1977, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[30]  I. Rasko,et al.  Caffeine enhancement of X-ray killing in cultured human and rodent cells. , 1978, Radiation research.

[31]  P. Deschavanne,et al.  Radiosensitivity and repair of radiation damage in human HF19 fibroblasts. , 1980, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[32]  R. Humphrey,et al.  The radiation response of human malignant melanoma cells grown in vitro. , 1971, Cancer research.

[33]  T. Puck,et al.  ACTION OF X-RAYS ON MAMMALIAN CELLS , 1956, The Journal of experimental medicine.

[34]  H. W. Jacox.  The action of radiation on living cells. , 1949, Radiology.

[35]  L. Zeitz.  Reported n and Dq values can be misleading. , 1979, The British journal of radiology.

[36]  R. Berry,et al.  Reproductive survival of explanted human tumor cells after exposure to nitrogen mustard or X irradiation; differences in response with subsequent subculture in vitro. , 1977, Radiation research.

[37]  E. Hall,et al.  Radiobiology for the radiologist , 1973 .

[38]  J. Fischer,et al.  Radiation therapy of malignant melanoma. Experience with high individual treatment doses , 1976 .

[39]  M. Bender,et al.  X-Ray-Induced Chromosome Aberrations and Reproductive Death in Mammalian Cells , 1961, The American Naturalist.

[40]  T. Inada,et al.  Comparative study on radiosensitivities of cultured cell lines derived from several human tumors under hypoxic condition. , 1977, Gan.

[41]  L. Tolmach,et al.  ANOMALOUS X-RAY SURVIVAL KINETICS IN HELA CELL POPULATIONS. , 1964, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[42]  L. Yang,et al.  Radiation response of cultured human carcinoembryonic antigen-producing colon adenocarcinoma cells. , 1977, International journal of radiation oncology, biology, physics.

[43]  F. Ellis.  The NSD concept and radioresistant tumours , 1974 .

[44]  M. Bender,et al.  The kinetics of x-ray survival of mammalian cells in vitro. , 1962, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[45]  R. Wideroee.  VARIOUS EXAMPLES FROM CELLULAR KINETICS SHOWING HOW RADIATION QUALITY CAN BE ANALYSED AND CALCULATED BY THE TWO-COMPONENT THEORY OF RADIATION. , 1971 .

[46]  A. Taylor,et al.  Ataxia telangiectasia: a human mutation with abnormal radiation sensitivity , 1975, Nature.

[47]  R. Berry,et al.  The effect of irradiated feeder cells on the X-ray survival curve shape of freshly explanted human tumor cells and a standard human tumor cell line. , 1980, Radiation research.

[48]  K H Chadwick,et al.  A molecular theory of cell survival. , 1973, Physics in medicine and biology.