Enhanced surface plasmon resonance imaging detection of DNA hybridization on periodic gold nanoposts.

We explore periodic gold nanoposts as substrates for the enhanced surface plasmon resonance imaging (SPRi) detection of DNA hybridization. Rigorous coupled-wave analysis was used to model and design the nanopost-based SPRi biosensor. Arrayed gold nanoposts on gold-coated glass substrate, with various widths and periodicity, were fabricated using electron-beam lithography and characterized with scanning electron and atomic force microscopy. A scanning-angle SPRi apparatus was used to conduct the kinetic analysis of DNA hybridization on nanopost-based sensor surface and assess the corresponding SPR signal amplification. Experimental results showed that both the nanostructure size and period influenced the SPR signal enhancement; the optimized 30 nm height, 50 nm size, and 110 nm period nanoposts provided a fivefold SPR signal amplification compared with the plain 50 nm thick gold film used as control.

[1]  R. Georgiadis,et al.  The effect of surface probe density on DNA hybridization. , 2001, Nucleic acids research.

[2]  S. J. Chen,et al.  Enhancement of the resolution of surface plasmon resonance biosensors by control of the size and distribution of nanoparticles. , 2004, Optics letters.

[3]  Donghyun Kim,et al.  Investigation of the profile effect on the sensitivity enhancement of nanowire-mediated localized surface plasmon resonance biosensors , 2006 .

[4]  Donghyun Kim,et al.  Effect of resonant localized plasmon coupling on the sensitivity enhancement of nanowire-based surface plasmon resonance biosensors. , 2006, Journal of the Optical Society of America. A, Optics, image science, and vision.

[5]  Donghyun Kim,et al.  Design study of highly sensitive nanowire-enhanced surface plasmon resonance biosensors using rigorous coupled wave analysis. , 2005, Optics express.

[6]  L. Chau,et al.  Sensing capability of the localized surface plasmon resonance of gold nanorods. , 2007, Biosensors & bioelectronics.

[7]  E. Hutter,et al.  Exploitation of Localized Surface Plasmon Resonance , 2004 .

[8]  Fredrik Höök,et al.  Improving the instrumental resolution of sensors based on localized surface plasmon resonance. , 2006, Analytical chemistry.

[9]  Suntak Park,et al.  Resonant coupling of surface plasmons to radiation modes by use of dielectric gratings. , 2003, Optics letters.

[10]  Lin He,et al.  Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization , 2000 .

[11]  A. Haes,et al.  A unified view of propagating and localized surface plasmon resonance biosensors , 2004, Analytical and bioanalytical chemistry.

[12]  Selim Elhadj,et al.  Optical properties of an immobilized DNA monolayer from 255 to 700 nm. , 2004, Langmuir : the ACS journal of surfaces and colloids.