Stokes shift spectroscopy for breast cancer diagnosis

The objective of this study is to assess the diagnostic potential of stokes shift (SS) spectroscopy (SSS) for normal and different pathological breast tissues such as fibroadenoma and infiltrating ductal carcinoma. The SS spectra is measured by simultaneously scanning both the excitation and emission wavelengths while keeping a fixed wavelength interval Δλ=20 nm between them. Characteristic, highly resolved peaks and significant spectral differences between normal and different pathological breast tissues were observed. The SS spectra of normal and different pathological breast tissues shows the distinct peaks around 300, 350, 450, 500 and 600 nm may be attributed to tryptophan, collagen, NADH, flavin and porphyrin respectively. Using SSS technique one can obtain all the key fluorophores in a single scan and hence they can be targeted as a tumor markers in this study. In order to quantify the altered spectral differences between normal and different pathological breast tissues are verified by different ratio parameters.

[1]  Alexey S. Ladokhin,et al.  Fluorescence Spectroscopy in Peptide and Protein Analysis , 2006 .

[2]  Nirmala Ramanujam,et al.  Diagnosis of breast cancer using fluorescence and diffuse reflectance spectroscopy: a Monte-Carlo-model-based approach. , 2008, Journal of biomedical optics.

[3]  B. Chance,et al.  Oxidation-reduction ratio studies of mitochondria in freeze-trapped samples. NADH and flavoprotein fluorescence signals. , 1979, The Journal of biological chemistry.

[4]  S. Thomsen,et al.  Physiological and Pathological Factors of Human Breast Disease That Can Influence Optical Diagnosis a , 1998, Annals of the New York Academy of Sciences.

[5]  Stavros G. Demos,et al.  Advances in Optical Spectroscopy and Imaging of Breast Lesions , 2006, Journal of Mammary Gland Biology and Neoplasia.

[6]  Robert R. Alfano,et al.  Optical spectroscopy of benign and malignant breast tissues , 1996, Photonics West.

[7]  Miroslav D. Dramićanin,et al.  Three-dimensional Total Synchronous Luminescence Spectroscopy Criteria for Discrimination Between Normal and Malignant Breast Tissues , 2005, Photochemistry and photobiology.

[8]  R. Alfano,et al.  Laser induced fluorescence spectroscopy from native cancerous and normal tissue , 1984 .

[9]  Joseph R. Lakowicz,et al.  Principles of Fluorescence Spectroscopy, Third Edition , 2008 .

[10]  Robert R. Alfano,et al.  Stokes shift emission spectroscopy of human tissue and key biomolecules , 2003 .

[11]  Singaravelu Ganesan,et al.  Synchronous Fluorescence Spectroscopy for the Detection and Characterization of Cervical Cancers In Vitro , 2010, Photochemistry and photobiology.

[12]  Haishan Zeng,et al.  Laser-induced autofluorescence microscopy of normal and tumor human colonic tissue. , 2004, International journal of oncology.

[13]  Zoya I. Volynskaya,et al.  Diagnosing breast cancer using diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopy. , 2008, Journal of biomedical optics.

[14]  Roberto Lenarduzzi,et al.  Development of a synchronous fluorescence imaging system and data analysis methods. , 2007, Optics express.

[15]  D. Choy,et al.  Fluorescence spectra from cancerous and normal human breast and lung tissues , 1987, Annual Meeting Optical Society of America.

[16]  Nirmala Ramanujam,et al.  Comparison of multiexcitation fluorescence and diffuse reflectance spectroscopy for the diagnosis of breast cancer (March 2003) , 2003, IEEE Transactions on Biomedical Engineering.

[17]  S. Lakhani,et al.  Diagnosis of breast cancer using elastic-scattering spectroscopy: preliminary clinical results. , 2000, Journal of biomedical optics.

[18]  Rebecca R. Richards-Kortum,et al.  Optimal excitation wavelengths for discrimination of cervical neoplasia , 2002, IEEE Transactions on Biomedical Engineering.

[19]  S. Majumder,et al.  Breast cancer diagnosis using N2 laser excited autofluorescence spectroscopy , 1997, Lasers in surgery and medicine.