Trithiane silver-nanoparticles-decorated polyaniline nanofibers as sensing element for electrochemical determination of Adenine and Guanine in DNA

Abstract Here, we report the synthesis of 1,3,5-Trithiane-Ag-nanoparticles decorated polyaniline nanofibers (TAN-AgNP-PANF) and describe its benefit in the sensing element of an electrochemical sensor for simultaneous detection of Adenine (A) and Guanine (G). We characterized the electrode composition with field emission-scanning electron microscopy (FE-SEM), atomic force microscopy (AFM) and Fourier-transform infrared spectroscopy (FT-IR). The electrocatalytic oxidation of A and G on the electrode sensor was explored by differential pulse voltammetry (DPV) and cyclic voltammetry (CV). This sensor shows separate and well-defined peaks for A and G, by which one can determine these biological bases individually or simultaneously. A linear relationship is figured out between the peak current and the analytes' concentrations on range 1.0 to 200 and 0.9 to 140 μM, for simultaneous determination of A and G, with detection limits of 2.8 and 3.0 μM, respectively. Furthermore, individually determination is resulted in two linear concentration ranges of 0.08 to 25.0 and 0.01 to 15.0 μM with detection limits of 24.0 and 0.06 μM for A and G, respectively. The sensor demonstrates a good stability and sufficient selectivity over the amino acids and could be used to quantify of A and G in fish sperm DNA sample successfully.

[1]  Wenwei Tang,et al.  An electrochemical sensor based on polyaniline for monitoring hydroquinone and its damage on DNA. , 2014, Talanta.

[2]  K. Schwarzová-Pecková,et al.  Sensitive voltammetric method for rapid determination of pyridine herbicide triclopyr on bare boron-doped diamond electrode , 2015 .

[3]  S. Basu,et al.  Interaction of DNA bases with silver nanoparticles: assembly quantified through SPRS and SERS. , 2008, Journal of colloid and interface science.

[4]  Y. Chu,et al.  Poly(alizarin red)/graphene modified glassy carbon electrode for simultaneous determination of purine and pyrimidine. , 2012, Analytica chimica acta.

[5]  E. Andrzejewska,et al.  Photochemistry of 1,3,5-trithianes in solution: Steady-state and laser flash photolysis studies , 2006 .

[6]  Xuping Sun,et al.  Ag nanoparticles decorated polyaniline nanofibers: synthesis, characterization, and applications toward catalytic reduction of 4-nitrophenol and electrochemical detection of H2O2 and glucose , 2012 .

[7]  M. Lapkowski,et al.  Cyclic voltammetry of polyaniline: interpretation of the middle peak , 1988 .

[8]  J. Rice,et al.  Mass spectra of nucleic acid derivatives. II. Guanine, adenine, and related compounds. , 1967, Journal of the American Chemical Society.

[9]  C. Speicher,et al.  Elevated nucleoside excretion by patients with nasopharyngeal carcinoma: Preliminary diagnostic/prognostic evaluations , 1982, Cancer.

[10]  G. Burnstock.  Purinergic Signaling and Vascular Cell Proliferation and Death , 2002, Arteriosclerosis, thrombosis, and vascular biology.

[11]  S. Yao,et al.  Determination of guanine and adenine by high-performance liquid chromatography with a self-fabricated wall-jet/thin-layer electrochemical detector at a glassy carbon electrode. , 2015, Talanta.

[12]  Cecie Starr,et al.  Biology: Concepts and Applications , 2005 .

[13]  R. Vajtai.  Springer Handbook of Nanomaterials , 2013 .

[14]  Wu Yang,et al.  Electrochemical behaviors and simultaneous determination of guanine and adenine based on graphene–ionic liquid–chitosan composite film modified glassy carbon electrode , 2012 .

[15]  Sanghamitra Chatterjee,et al.  Nanomaterials based electrochemical sensors for biomedical applications. , 2013, Chemical Society reviews.

[16]  M. Re,et al.  The behavior of free purine nucleotides in lymphocytes infected with HIV-1 virus. , 1993, Biochimica et biophysica acta.

[17]  Geoffrey Burnstock,et al.  Roles of P2‐Purinoceptors in the Cardiovascular System , 1991, Circulation.

[18]  W. Nyhan.  Disorders of purine and pyrimidine metabolism. , 2005, Molecular genetics and metabolism.

[19]  Jianping Li,et al.  A sensitive electrochemical molecularly imprinted sensor based on catalytic amplification by silver nanoparticles for 3-indoleacetic acid determination , 2014 .

[20]  I. Fox,et al.  Metabolic basis for disorders of purine nucleotide degradation. , 1981, Metabolism: clinical and experimental.

[21]  J. Harmenberg,et al.  Urinary modified nucleosides as tumor markers in cancer of the urinary organs or female genital tract , 1986, Urological research.

[22]  G. Burnstock.  The past, present and future of purine nucleotides as signalling molecules , 1997, Neuropharmacology.

[23]  K. Jiao,et al.  Highly sensitive and synergistic detection of guanine and adenine based on poly(xanthurenic acid)-reduced graphene oxide interface. , 2014, ACS applied materials & interfaces.

[24]  Himansu S. Biswal,et al.  Nature of the N-H...S hydrogen bond. , 2009, The journal of physical chemistry. A.

[25]  W. Baeyens,et al.  Separation of purine and pyrimidine bases by ion chromatography with direct conductivity detection. , 2008, Journal of chromatography. A.

[26]  N. B. Luque,et al.  Ab initio studies of Ag-S bond formation during the adsorption of L-cysteine on Ag(111). , 2012, Langmuir : the ACS journal of surfaces and colloids.

[27]  Xiao-fei Zhu,et al.  Overoxidized polypyrrole/graphene nanocomposite with good electrochemical performance as novel electrode material for the detection of adenine and guanine. , 2014, Biosensors & bioelectronics.

[28]  I. Heisler,et al.  A colorimetric assay for the quantitation of free adenine applied to determine the enzymatic activity of ribosome-inactivating proteins. , 2002, Analytical biochemistry.

[29]  Yong Wang,et al.  Facile synthesis of polyaniline nanofibers using chloroaurate acid as the oxidant. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[30]  J. Davidson.  The biochemistry of the nucleic acids , 1950 .

[31]  A. C. Pandey,et al.  A new class of PANI-Ag core-shell nanorods with sensing dimensions. , 2012, Nanoscale.

[32]  Wolfgang Buchberger,et al.  Investigations on the migration behaviour of purines and pyrimidines in capillary electromigration techniques with UV detection and mass spectrometric detection. , 2008, Journal of chromatography. A.

[33]  Kai Zhang,et al.  Graphene/Polyaniline Nanofiber Composites as Supercapacitor Electrodes , 2010 .

[34]  Zonghua Wang,et al.  β-Cyclodextrin incorporated carbon nanotubes-modified electrodes for simultaneous determination of adenine and guanine , 2006 .