A Conserved Gene Cluster Rules Anaerobic Oxidative Degradation of L-Ornithine

ABSTRACT For the ornithine fermentation pathway, described more than 70 years ago, genetic and biochemical information are still incomplete. We present here the experimental identification of the last four missing genes of this metabolic pathway. They encode l-ornithine racemase, (2R,4S)-2,4-diaminopentanoate dehydrogenase, and the two subunits of 2-amino-4-ketopentanoate thiolase. While described only for the Clostridiaceae to date, this pathway is shown to be more widespread.

[1]  Georges N. Cohen,et al.  “Candidatus Cloacamonas Acidaminovorans”: Genome Sequence Reconstruction Provides a First Glimpse of a New Bacterial Division , 2008, Journal of bacteriology.

[2]  Claudine Médigue,et al.  Identification of the Last Unknown Genes in the Fermentation Pathway of Lysine* , 2007, Journal of Biological Chemistry.

[3]  William T. Self,et al.  Analysis of Proline Reduction in the Nosocomial Pathogen Clostridium difficile , 2006, Journal of bacteriology.

[4]  C. Médigue,et al.  MaGe: a microbial genome annotation system supported by synteny results , 2006, Nucleic acids research.

[5]  K. Mori,et al.  Identification of a Reactivating Factor for Adenosylcobalamin-Dependent Ethanolamine Ammonia Lyase , 2004, Journal of bacteriology.

[6]  Shih-Hsiung Wu,et al.  Cloning, Sequencing, Heterologous Expression, Purification, and Characterization of Adenosylcobalamin-dependentd-Ornithine Aminomutase from Clostridium sticklandii * , 2001, The Journal of Biological Chemistry.

[7]  Shih-Hsiung Wu,et al.  Purification and Properties of Ornithine Racemase from Clostridium sticklandii , 2000, Journal of bacteriology.

[8]  J. E. Bell,et al.  Regulation of bovine glutamate dehydrogenase. The effects of pH and ADP. , 1982, The Journal of biological chemistry.

[9]  T. Bücher,et al.  Molar absorptivities of beta-NADH and beta-NADPH. , 1976, Clinical chemistry.

[10]  H. A. Barker,et al.  Ornithine degradation in Clostridium sticklandii; pyridoxal phosphate and coenzyme A dependent thiolytic cleavage of 2-amino-4-ketopentanoate to alanine and acetyl coenzyme A. , 1974, Biochemistry.

[11]  R. Somack,et al.  Purification and properties of a pyridoxal phosphate and coenzyme B 12 dependent D- -ornithine 5,4-aminomutase. , 1973, Biochemistry.

[12]  R. Somack,et al.  2,4-diaminopentanoic acid C 4 dehydrogenase. Purification and properties of the protein. , 1973, The Journal of biological chemistry.

[13]  H. Friedmann,et al.  Ornithine metabolism by Clostridium sticklandii. Oxidation of ornithine to 2-amino-4-ketopentanoic acid via 2,4-diaminopentanoic acid; participation of B12 coenzyme, pyridoxal phosphate, and pyridine nucleotide. , 1970, The Journal of biological chemistry.

[14]  R. N. Costilow,et al.  2,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine by Clostridium sticklandii , 1970, Journal of bacteriology.

[15]  R. N. Costilow,et al.  Fermentation of Ornithine by Clostridium sticklandii , 1968, Journal of bacteriology.

[16]  R. N. Costilow,et al.  PHYSIOLOGY OF THE SPORULATION PROCESS IN CLOSTRIDIUM BOTULINUM. II. MATURATION OF FORESPORES. , 1964, Journal of bacteriology.

[17]  R. N. Costilow,et al.  PHYSIOLOGY OF THE SPORULATION PROCESS IN CLOSTRIDIUM BOTULINUM I , 1964, Journal of bacteriology.

[18]  R. N. Costilow.  FERMENTATIVE ACTIVITIES OF CONTROL AND RADIATION-“KILLED” SPORES OF CLOSTRIDIUM BOTULINUM , 1962, Journal of bacteriology.

[19]  D. D. Woods.  Studies in the metabolism of the strict anaerobes (genus Clostridium): Further experiments on the coupled reactions between pairs of amino-acids induced by Cl. sporogenes. , 1936, The Biochemical journal.

[20]  L. H. Stickland.  Studies in the metabolism of the strict anaerobes (genus Clostridium): The oxidation of alanine by Cl. sporogenes. IV. The reduction of glycine by Cl. sporogenes. , 1935, The Biochemical journal.

[21]  Genus Clostridium,et al.  STUDIES IN THE METABOLISM OF THE STRICT ANAEROBES ( GENUS CLOSTRIDIUM ) . V . FURTHER EXPERIMENTS ON THE COUPLED REACTIONS BETWEEN PAIRS OF AMINO-ACIDS INDUCED BY CL , 2005 .

[22]  E. Stadtman.  [137] Preparation and assay of acyl coenzyme A and other thiol esters; use of hydroxylamine , 1957 .

[23]  L. H. Stickland.  Studies in the metabolism of the strict anaerobes (genus Clostridium): The chemical reactions by which Cl. sporogenes obtains its energy. , 1934, The Biochemical journal.