Sarcoplasmic reticulum Ca2+ uptake and leak properties, and SERCA isoform expression, in type I and type II fibres of human skeletal muscle

Release and uptake of Ca2+ ions by the sarcoplasmic reticulum (SR) regulates contraction in skeletal muscle. SR Ca2+ uptake and leak properties in human muscle are presently not well defined. The surface membrane of individual human muscle fibres was removed by microdissection, and the rate of SR Ca2+ uptake at different applied [Ca2+] assessed from the amount of Ca2+ accumulated. Ca2+ uptake occurred at lower [Ca2+] in type I fibres than in type II fibres, consistent with the contractile apparatus properties in the respective fibre types. Maximal uptake rate was slightly greater in type II fibres, and approximately two Ca2+ were taken up per ATP hydrolysed. Ca2+ leaking out of the SR ultimately has to be pumped back in again at the cost of ATP usage. SR Ca2+ leakage in human muscle fibres was smaller and regulated differently to that in rat muscle fibres, probably reflecting different contributions to thermogenesis.

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