论文信息 - Third harmonic generation microscopy.

Third harmonic generation microscopy.

Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 aem results in a 400 nm signal which is generated directly within the specimen. Chara plant rhizoids have been imaged, showing dynamic plant activity, and non-fading image characteristics even with continuous viewing, indicating prolonged viability under these THG-imaging conditions.

[1] D. Hodick,et al. Gravity sensing in tip-growing cells. , 1996, Trends in plant science.

[2] Yaron Silberberg,et al. Nonlinear scanning laser microscopy by third harmonic generation , 1997 .

[3] J. Squier,et al. Dispersion pre‐compensation of 15 femtosecond optical pulses for high‐numerical‐aperture objectives , 1998, Journal of microscopy.

[4] T. Tsang. Reflected optical harmonics from dielectric mirrors. , 1994, Applied optics.

[5] J. Pawley,et al. Handbook of Biological Confocal Microscopy , 1990, Springer US.

[6] Wilson,et al. 3D microscopy of transparent objects using third‐harmonic generation , 1998, Journal of microscopy.

[7] Tsang,et al. Optical third-harmonic generation at interfaces. , 1995, Physical review. A, Atomic, molecular, and optical physics.