Associative Nitrogen Fixation in Lowland Rice

Nitrogen (N), a most limiting nutrient, is the input required in the largest quantity for lowland rice production. The concerns on N economy and efficiency and its impact on environment have renewed interest in exploring alternative or supplementary N source for sustainable agriculture. Several studies have indicated the existence of significant rice genotypic differences in N2 fixation stimulating traits (NFS). Rice genotypes with high NFS are desirable because they add N to the soil-water-plant system without additional farm inputs and reduce dependence on fertilizer. Large genotypic differences in percent N derived from air (% Ndfa) like 1.5% in Abang Basur, medium maturing genotype, to 21% in Oking Seroni, late maturing genotype, indicates potential of isolating genotypes with high NFS for sustainable agriculture. The exogenous supply of nitrogenous fertilizer to lowland rice significantly inhibited N fixation but improved plant growth. Where as phosphorous fertilizer did not affect atom % 15 N excess and % Ndfa significantly but slight decrease in atom % 15 N excess and increase in N 2 fixation was observed. Inhibitory effect of exogenous supply of N fertilizer indicates limited potential of associative N 2 fixation to significantly benefit agriculture. Farmers would have to withhold N fertilizer from their rice crop in order to increase biological N 2 -fixation associated with rice. If they do such practice the plants will be N deficient and might have a lower yield. However, the development of N fixation in response to a deficiency of available N may well be an integral part of the N cycle of natural ecosystem and low input farming system there by maintaining a N balance in the environment. Key words: Fertilizer; fixation; nitrogen; rice DOI: 10.3126/narj.v6i0.3373 Nepal Agriculture Research Journal Vol.6 2005 pp.110-111

[1]  J. Döbereiner,et al.  Nitrogen fixation associated with grasses and cereals: Recent results and perspectives for future research , 1988, Plant and Soil.

[2]  T. Yoneyama,et al.  Effect of inoculation withKlebsiella oxytoca andEnterobacter cloacae on dinitrogen fixation by rice-bacteria associations , 1987, Plant and Soil.

[3]  N. Sanginga.  Early growth and N2-fixation of leucaena and gliricidia at different levels of phosphorus application , 1992, Fertilizer research.

[4]  I. Watanabe.  Nitrogen fixation by non-legumes in tropical agriculture with special reference to wetland rice , 1986, Plant and Soil.

[5]  J. Ladha,et al.  Laboratory acetylene reduction assay for relative measurement of N2-fixing activities associated with field-grown wetland rice plants , 1986, Plant and Soil.

[6]  I. Watanabe,et al.  The effect of nitrogen fertilizer on N2 fixation in the paddy field measured byin situ acetylene reduction assay , 1981, Plant and Soil.

[7]  R. Johnson,et al.  Mineral Nutrition , 2019, World Soybean Research Conference II: Abstracts.

[8]  F. Leganés,et al.  Epiphytic Cyanobacteria on Chara vulgaris Are the Main Contributors to N2 Fixation in Rice Fields , 2004, Applied and Environmental Microbiology.

[9]  J. Ladha,et al.  Nitrogen fixation in rice systems: state of knowledge and future prospects , 2003, Plant and Soil.

[10]  J. Schjoerring,et al.  Measurement of biological dinitrogen fixation in grassland: Comparison of the enriched 15N dilution and the natural 15N abundance methods at different nitrogen application rates and defoliation frequencies , 1994, Plant and Soil.

[11]  T. Yoneyama,et al.  Field evaluation of nitrogen fixation and use of nitrogen fertilizer by sorghum/pigeonpea intercropping on an Alfisol in the Indian semi-arid tropics , 1994, Biology and Fertility of Soils.

[12]  P. Chalk.  The contribution of associative and symbiotic nitrogen fixation to the nitrogen nutrition of non-legumes , 1991, Plant and Soil.

[13]  F. Zapata,et al.  Measurements of nitrogen fixation in fababean at different N fertilizer rates using the 15N isotope dilution and ‘A-value’ methods , 1991, Plant and Soil.

[14]  G. Trolldenier.  Estimation of associative nitrogen fixation in relation to water regime and plant nutrition in a long-term pot experiment with rice (Oryza sativa L.) , 1987, Biology and Fertility of Soils.

[15]  H. G. Diem,et al.  Inoculation withGlomus mosseae improves N2 fixation by field-grown soybeans , 1985, Biology and Fertility of Soils.

[16]  Tomio Yoshida,et al.  Nitrogen fixation throughout growth, and varietal differences in nitrogen fixation by the rhizosphere of rice planted in pots , 1977, Plant and Soil.

[17]  G. Trolldenier.  Influence of some environmental factors on nitrogen fixation in the rhizosphere of rice , 1977, Plant and Soil.

[18]  G. Trolldenier.  Secondary effects of potassium and nitrogen nutrition of rice: Change in microbial activity and iron reduction in the rhizosphere , 1973, Plant and Soil.

[19]  H. G. Diem,et al.  Comparative growth and symbiotic performance of four Acacia mangium provenances from Papua New Guinea in response to the supply of phosphorus at various concentrations , 2004, Biology and Fertility of Soils.

[20]  I. Kennedy,et al.  Prospects and potentials for systems of biological nitrogen fixation in sustainable rice production , 2004, Biology and Fertility of Soils.

[21]  T. Adhya,et al.  Review: Current status and future prospects of associative nitrogen fixation in rice , 1998 .

[22]  J. Ladha,et al.  Genotypic Variation in Promotion of Rice Dinitrogen Fixation as Determined by Nitrogen‐15 Dilution , 1996 .

[23]  D. K. Barnes,et al.  Ineffectively and Effectively Nodulated Alfalfas Demonstrate Biology Nitrogen Fixation Continues with High Nitrogen Fertilization , 1995 .

[24]  J. Duxbury,et al.  Inorganic nitrogen supply and symbiotic dinitro‐gen fixation in alfalfa , 1994 .

[25]  K. Mengel.  Symbiotic dinitrogen fixation ‐ its dependence on plant nutrition and its ecophysiological impact , 1994 .

[26]  J. Nösberger,et al.  Effects of phosphorus and potassium on N2 fixation (15N-dilution) of field-grown Centrosema acutifolium and C. macrocarpum , 1993 .

[27]  W. Song,et al.  Association of Alcaligenes faecalis with wetland rice , 1991 .

[28]  E. S. Jensen,et al.  Dinitrogen fixation and soil n uptake by soybean as affected by phosphorus availability , 1991 .

[29]  J. Ladha,et al.  Effect of inorganic N and organic fertilizers on nitrogen-fixing (acetylene-reducing) activity associated with wetland rice plants , 1989 .

[30]  J. Ladha,et al.  A plant sampling procedure for acetylene reduction assay to detect rice varietal differences in ability to stimulate N2 fixation , 1988 .

[31]  J. Ladha,et al.  Difference in Natural Abundance of 15N in Several Rice (Oryza sativa L.) Varieties: Application for Evaluating N2 Fixation , 1987 .

[32]  D. Israel,et al.  Investigation of the role of phosphorus in symbiotic dinitrogen fixation. , 1987, Plant physiology.

[33]  J. Ladha,et al.  Nitrogen-Fixing (C2H2-Reducing) Activity and Plant Growth Characters of 16 Wetland Rice Varieties , 1987 .

[34]  T. Ventura,et al.  THE EFFECT OF CULTIVATED AND WILD RICE VARIETIES ON THE NITROGEN BALANCE OF FLOODED SOIL , 1986 .

[35]  T. Yoneyama,et al.  Dinitrogen Fixation of Rice-Klebsiella Associations 1 , 1986 .

[36]  P. Roger,et al.  Technologies for utilizing biological nitrogen fixation in wetland rice: potentialities, current usage, and limiting factors , 1986 .

[37]  J. Ladha,et al.  Plant-Associated N2 Fixation (C2H2-Reduction) by Five Rice Varieties, and Relationship with Plant Growth Characters as Affected by Straw Incorporation , 1986 .

[38]  I. Jakobsen.  The role of phosphorus in nitrogen fixation by young pea plants (Pisum sativum) , 1985 .

[39]  I. Watanabe,et al.  Response of wetland rice to inoculation with azospirillum lipoferum and pseudomonas Sp. , 1984 .

[40]  G. H. Heichel,et al.  Dinitrogen fixation of soybean and alfalfa: comparison of the isotope dilution and difference methods. , 1984 .

[41]  G. H. Heichel,et al.  Photosynthate Partitioning in Relation to N 2 Fixation Capability of Alfalfa 1 , 1983 .

[42]  W. Ventura,et al.  15N Dilution technique of assessing the contribution of nitrogen fixation to rice plant , 1983 .

[43]  P. van Berkum,et al.  Ontogenetic Variation of Nitrogenase, Nitrate Reductase, and Glutamine Synthetase Activities in Oryza sativa. , 1981, Plant physiology.

[44]  A. Eaglesham,et al.  Heterotrophic n(2) fixation and distribution of newly fixed nitrogen in a rice-flooded soil system. , 1981, Plant physiology.

[45]  P. Charyulu,et al.  INFLUENCE OF AMMONIUM NITROGEN ON NITROGEN FIXATION IN PADDY SOILS , 1981 .

[46]  T. Yoneyama,et al.  Atmospheric dinitrogen fixation in the flooded rice rhizosphere as determined by the N-15 isotope technique , 1980 .

[47]  M. Alexander,et al.  NONSYMBIOTIC NITROGEN FIXATION ASSOCIATED WITH THE RICE PLANT IN FLOODED SOILS , 1980 .

[48]  I. Watanabe,et al.  Fixation of Dinitrogen-15 Associated with Rice Plants , 1980, Applied and environmental microbiology.

[49]  Kurt Stockinger,et al.  Root Growth and Dry Matter Distribution of Soybean as Affected by Phosphorus Stress, Nodulation, and Nitrogen Source 1 , 1980 .

[50]  P. Charyulu,et al.  Influence of various soil factors on nitrogen fixation by Azospirillum spp. , 1980 .

[51]  P. Roger,et al.  Blue-green algae and rice , 1980 .

[52]  E. Dunigan,et al.  Non-symbiotic nitrogen fixation in rice. , 1980 .

[53]  W. Barraquio,et al.  Nitrogen-Fixing (Acetylene Redution) Activity and Population of Aerobic Heterotrophic Nitrogen-Fixing Bacteria Associated with Wetland Rice , 1979, Applied and environmental microbiology.

[54]  R. Knowles,et al.  Effect of oxygen and nitrate on nitrogen fixation and denitrification by Azospirillum brasilense grown in continuous culture. , 1978, Canadian journal of microbiology.

[55]  Y. Sano,et al.  Nitrogen fixation in the rhizosphere of rice , 1978, Nature.

[56]  V. Rajaramamohan-Rao.  Nitrogen fixation as influenced by moisture content, ammonium sulphate and organic sources in a paddy soil , 1976 .

[57]  R. Knowles,et al.  Effect of ammonium-, nitrite- and nitrate nitrogen on anaerobic nitrogenase activity in soil , 1974 .

[58]  Tomio Yoshida,et al.  Nitrogen-Fixing Activity in Upland and Flooded Rice Fields 1 , 1973 .

[59]  Tomio Yoshida,et al.  Nitrogen Fixation by Bacteria in the Root Zone of Rice , 1971 .

[60]  D. S. Chamblee,et al.  Influence of Inorganic Nitrogen on Nitrogen Fixation by Legumes as Revealed by N151 , 1958 .

[61]  '. I.Watanabe.  Nitrogen Fixation in Wetland Rice Field , 2022 .