Pathogenicity by Parenteral Injection of Fowl Adenovirus Isolated from Gizzard Erosion and Resistance to Reinfection in Adenoviral Gizzard Erosion in Chickens

The pathogenicity of a serotype-1 fowl adenovirus (FAV-99ZH), which causes adenoviral gizzard erosion by oral inoculation in chickens, was investigated in specific pathogen-free white leghorn chickens. In trial 1, 14 chickens were inoculated intravenously with the virus at 21 days of age and euthanatized for necropsy within 1–14 days of inoculation. Gizzard erosion was grossly observed from day 7 postinoculation (PI), and histologically, FAV-99ZH antigen-positive, basophilic intranuclear inclusion bodies were seen in the gizzard lesions from day 7 to 11 PI. Necrotizing pancreatitis, and cholecystitis and cholangitis associated with the inclusions were observed from day 3 to 14 PI (pancreatitis) and from day 5 to 9 PI (cholecystitis and cholangitis), respectively. The inclusions were also observed in the epithelial cells of the cecal tonsils from day 3 to 5 PI. The virus was recovered from samples of the lesions. It was revealed that FAV-99ZH causes not only gizzard erosion but also pancreatitis, cholecystitis, and cholangitis by intravenous inoculation in chickens. In trial 2, 10 chickens were inoculated orally with the virus twice, at 13 and 36 days of age, and euthanatized for necropsy within 4–17 days after reinfection. Macroscopically, focal gizzard lesions were observed; however, neither necrosis nor inclusions were observed by microscopy. Moreover, FAV was not recovered from the gizzard or rectum of any of the chickens at necropsy. This suggests that the gizzard lesions occurred as a result of the primary infection, and that the chickens were able to resist reinfection.

[1]  K. Okada,et al.  Outbreaks of adenoviral gizzard erosion in slaughtered broiler chickens in Japan , 2003, Veterinary Record.

[2]  K. Okada,et al.  Adenoviral Gizzard Erosion in Commercial Broiler Chickens , 2003, Veterinary pathology.

[3]  M. Mase,et al.  Experimental Gizzard Erosions in Specific-Pathogen-Free Chicks by Serotype 1 Group I Avian Adenoviruses from Broilers , 2002, Avian diseases.

[4]  K. Nakamura,et al.  Pancreatic Necrosis and Ventricular Erosion in Adenovirus-associated Hydropericardium Syndrome of Broilers , 2002, Veterinary pathology.

[5]  S. Sato,et al.  Pathogenicity of serotype 1 fowl adenovirus in commercial broiler chickens. , 2001, Avian diseases.

[6]  K. Nakamura,et al.  Gizzard erosion in broiler chicks by group I avian adenovirus. , 2001, Avian diseases.

[7]  S. Sato,et al.  Experimental infection of specific-pathogen-free chickens with serotype-1 fowl adenovirus isolated from a broiler chicken with gizzard erosions. , 2001, Avian diseases.

[8]  M. Haritani,et al.  Epizootic outbreaks of gizzard erosion associated with adenovirus infection in chickens. , 2001, Avian diseases.

[9]  K. Okada,et al.  Cholangiohepatitis in broiler chickens in Japan: histopathological, immunohistochemical and microbiological studies of spontaneous disease. , 2000, Acta veterinaria Hungarica.

[10]  M. Vyberg,et al.  Dextran Polymer Conjugate Two-Step Visualization System for Immunohistochemistry: A Comparison of EnVision+ With Two Three-Step Avidin-Biotin Techniques , 1998 .

[11]  M. Hess,et al.  Hexon based PCRs combined with restriction enzyme analysis for rapid detection and differentiation of fowl adenoviruses and egg drop syndrome virus. , 1998, Journal of virological methods.

[12]  H. Tojo,et al.  Histology, immunohistochemistry, and ultrastructure of hydropericardium syndrome in adult broiler breeders and broiler chicks. , 1998, Avian diseases.

[13]  F. Hoerr,et al.  Gastrointestinal Pathogenicity of Adenoviruses and Reoviruses Isolated from Broiler Chickens in Alabama , 1998, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[14]  M. Goodwin.  Adenovirus inclusion body ventriculitis in chickens and captive bobwhite quail (Colinus virginianus). , 1993, Avian diseases.

[15]  K. Nakamura,et al.  Necrotizing pancreatitis and gizzard erosion associated with adenovirus infection in chickens. , 1993, Avian diseases.

[16]  D. L. Hill,et al.  Multisystemic adenovirus infection in broiler chicks with hypoglycemia and spiking mortality. , 1993, Avian diseases.

[17]  P. Szeleszczuk,et al.  Nadzerki blony rogowej mielca [gizzard erosion] u zarodkow i pisklat kurzych , 1993 .

[18]  A. Dhillon,et al.  Adenovirus infection associated with respiratory disease in commercial chickens. , 1987, Avian diseases.

[19]  H. Farmer,et al.  Pancreatic duct obstruction in a stunting syndrome of broiler chickens , 1986, Veterinary Record.

[20]  G. Wilcox,et al.  Runting syndrome in broiler chickens in Australia , 1982, Veterinary Record.

[21]  O. Fletcher,et al.  Comparative Study of Experimental Inclusion Body Hepatitis of Chickens Caused by Two Serotypes of Avian Adenovirus , 1978, Veterinary pathology.

[22]  V. J. Yates,et al.  Serological response of chickens exposed to a type 1 avian adenovirus alone or in combination with the adeno-associated virus. , 1977, Avian diseases.

[23]  King Dj,et al.  Virus-neutralizing antibody titers against 8 avian adenovirus serotypes in breeder hens in Georgia by a microneutralization procedure. , 1977 .

[24]  J. Mcferran,et al.  Isolation of viruses from clinical outbreaks of inclusion body hepatitis. , 1976, Avian pathology : journal of the W.V.P.A.

[25]  A. Fadly,et al.  Adenovirus infection and disease. II. Histopathology of natural and experimental disease. , 1973, Avian diseases.

[26]  D. Clemmer.  Experimental Enteric Infection of Chickens with an Avian Adenovirus (Strain 93) ∗ , 1965, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.