PHYLOGENETIC ANALYSIS REVEALS GENETIC VARIATIONS OF DENSOVIRUS ISOLATED FROM FIELD MOSQUITOES IN BANGKOK AND SURROUNDING REGIONS.

Screening for densoviruses (DNVs) from Aedes, Culex and Toxorhynchites mosquitoes collected in Bangkok and surrounding regions identified two clades of Aedes DNV; Ae. aegypti DNV (AaeDNV) and Ae. albopictus DNV (AalDNV) by PCR-restriction fragment length polymorphism (PCR-RFLP). From nucleotide sequencing and phylogenetic analysis of PCR amplicons of a fragment of DNV capsid gene, these DNVs were shown to be new DNV genetic variations similar to AaeDNV. Isolation and identification of densoviruses from indigenous field mosquitoes reside in natural habitat should be helpful in monitoring the distribution of DNVs in important mosquitoes, especially Ae. aegypti and Ae. albopictus, vector for dengue and yellow fever viruses.

[1]  A. Papa,et al.  Detection of West Nile virus and insect-specific flavivirus RNA in Culex mosquitoes, central Macedonia, Greece. , 2014, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[2]  E. Gould,et al.  Novel flaviviruses from mosquitoes: Mosquito-specific evolutionary lineages within the phylogenetic group of mosquito-borne flaviviruses , 2014, Virology.

[3]  Jorge E. Osorio-Benitez,et al.  Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes. , 2014, Journal of infection in developing countries.

[4]  K. Boonnak,et al.  Application of post-PCR methods for analysis of mosquito densovirus. , 2014, The Southeast Asian journal of tropical medicine and public health.

[5]  Oumar Faye,et al.  Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes , 2013, Virology Journal.

[6]  S. Pattanakitsakul,et al.  Comparison of mosquito densoviruses: two clades of viruses isolated from indigenous mosquitoes. , 2013, The Southeast Asian journal of tropical medicine and public health.

[7]  G. Stentiford.  Diseases in aquatic crustaceans: problems and solutions for global food security. , 2012, Journal of invertebrate pathology.

[8]  T. Flegel.  Historic emergence, impact and current status of shrimp pathogens in Asia. , 2012, Journal of invertebrate pathology.

[9]  A. Sivaram,et al.  Evidence of co-infection of chikungunya and densonucleosis viruses in C6/36 cell lines and laboratory infected Aedes aegypti (L.) mosquitoes , 2010, Parasites & Vectors.

[10]  T. Flegel,et al.  Persistent, triple-virus co-infections in mosquito cells , 2010, BMC Microbiology.

[11]  P. Yadav,et al.  Isolation and Characterization of Densonucleosis Virus from Aedes aegypti Mosquitoes and Its Distribution in India , 2009, Intervirology.

[12]  P. Fournier,et al.  Densovirus Infectious Pathway Requires Clathrin-Mediated Endocytosis Followed by Trafficking to the Nucleus , 2009, Journal of Virology.

[13]  P. Kittayapong,et al.  Mosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection. , 2008, Virology.

[14]  T. Flegel,et al.  Mosquito cells accommodate balanced, persistent co-infections with a densovirus and Dengue virus. , 2008, Developmental and comparative immunology.

[15]  H. Attoui,et al.  Isolation and characterization of the full coding sequence of a novel densovirus from the mosquito Culex pipiens pallens. , 2008, The Journal of general virology.

[16]  K. Boonnak,et al.  A new densovirus isolated from the mosquito Toxorhynchites splendens (Wiedemann) (Diptera: Culicidae). , 2007, The Southeast Asian journal of tropical medicine and public health.

[17]  H. Jiang,et al.  Genetic engineering of Periplaneta fuliginosa densovirus as an improved biopesticide , 2007, Archives of Virology.

[18]  Wei Wei,et al.  The pathogenicity of mosquito densovirus (C6/36DNV) and its interaction with dengue virus type II in Aedes albopictus. , 2006, The American journal of tropical medicine and hygiene.

[19]  J. Carlson,et al.  Densoviruses for control and genetic manipulation of mosquitoes. , 2006, Advances in virus research.

[20]  J. Carlson,et al.  Mosquito densonucleosis viruses cause dramatically different infection phenotypes in the C6/36 Aedes albopictus cell line. , 2005, Virology.

[21]  Rodriguez-Tan Rs,et al.  Dengue: a review. , 1998 .

[22]  Duane J. Gubler,et al.  Dengue and Dengue Hemorrhagic Fever , 1998, Clinical Microbiology Reviews.

[23]  R. Tesh,et al.  Insect densoviruses may be widespread in mosquito cell lines. , 1995, The Journal of general virology.

[24]  B. Beaty,et al.  Densovirus of Aedes aegypti as an expression vector in mosquito cells. , 1994, Experimental parasitology.

[25]  F. Jousset,et al.  Complete nucleotide sequence and genomic organization of the Aedes albopictus parvovirus (AaPV) pathogenic for Aedes aegypti larvae. , 1994, Virology.

[26]  M. Cornet,et al.  A parvo-like virus persistently infecting a C6/36 clone of Aedes albopictus mosquito cell line and pathogenic for Aedes aegypti larvae. , 1993, Virus research.

[27]  M. Jourdan,et al.  Complete nucleotide sequence of the cloned infectious genome of Junonia coenia densovirus reveals an organization unique among parvoviruses. , 1992, Virology.

[28]  C. Giraud,et al.  The densovirus of Junonia coenia (Jc DNV) as an insect cell expression vector. , 1992, Virology.

[29]  D. Gubler,et al.  Dengue and Dengue Hemorrhagic Fever , 1998, Clinical Microbiology Reviews.

[30]  E. Galyov,et al.  Nucleotide sequence and genomic organization of Aedes densonucleosis virus. , 1991, Virology.

[31]  Restriction maps and sequence homologies of two densovirus genomes. , 1990, The Journal of general virology.

[32]  P. Tattersall,et al.  Characteristics and taxonomy of Parvoviridae. , 1985, Intervirology.

[33]  Yiau-Min Huang.  The mosquitoes of Polynesia with a pictorial key to some species associated with filariasis and/or dengue fever. , 1977 .

[34]  E. Kurstak,et al.  Small DNA densonucleosis virus (DNV). , 1972, Advances in virus research.