A procedure for selecting specific areas of sections for comparative light and electron microscopic studies

The paper describes a method which enables correlative light and electron microscopical studies to be made of specific tissue structures or of individual cells. For this purpose a new specimen marker has been constructed. The device, which is attached to the objective of a light microscope, permits the operator to examine and exactly encircle the selected area without changing the objective. The technique is illustrated by two examples using immunoelectronmicroscopy.

[1]  R. Matre,et al.  Localization of the C3b receptors in human renal glomeruli. , 2009, Acta pathologica et microbiologica Scandinavica. Section C, Immunology.

[2]  R. Matre,et al.  Ultrastructural and immuno-electron microscopic study in a case of epidermolysis bullosa acquisita. , 2009, Acta pathologica et microbiologica Scandinavica. Section A, Pathology.

[3]  R. Nilsen,et al.  Enzyme histochemical studies of induced heterotopic cartilage and bone formation in guinea pigs with special reference to acid phosphatase. , 1981, Scandinavian journal of dental research.

[4]  W. Seliger,et al.  A new technique for the rapid screening and selection of large pieces of tissue for ultrastructural evaluation. , 1980, Stain technology.

[5]  W. Hopkins.  Vertical plane serial sectioning of identified tissue culture cells , 1979, Journal of microscopy.

[6]  P. Morgan,et al.  Correlation of histology and ultrastructure in pathology specimens. , 1978, Journal of oral pathology.

[7]  P. Chapman,et al.  A light microscope attachment for isolating small specific areas from slide embedded monolayers: an aid in electron microscopy , 1977, Journal of microscopy.

[8]  M. A. Gorycki,et al.  Improved technique for electron microscopy of cultured cells. , 1977, Stain technology.

[9]  R. Hauser.  A fast and simple method for the preparation of histologic cryostat sections for ultrastructural investigation. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  W. Ewijk,et al.  A new method for comparative light and electron microscopic studies of individual cells, selected in the living state , 1975, Journal of microscopy.

[11]  Buckley Ik.  A simple technique for comparative light and electron microscopy of designated living cultured cells. , 1971 .

[12]  B. Brinkley,et al.  PROCEDURE FOR EMBEDDING IN SITU SELECTED CELLS CULTURED IN VITRO , 1967, The Journal of cell biology.