Characterization of Sardinian cork oak (Quercus suber L.) genetic resources for economically important traits

Considering the very high socio economic value of cork oak (Quercus suber L.) in Mediterranean areas, a better knowledge of local genetic resources is necessary in order to reach a good balance between the conservation issues and the need of an efficient cork production network. In such frame, local germplasm of Q. suber from the main cork production areas of Sardinia was analysed for DNA and isozyme polymorphisms and for cork quality in relation to some environmental parameters. A total number of 24 sample stands of cork oak were selected, representing typical vegetation, sylvicultural and cork quality features within the eight identified cork growing areas in Sardinia. Considerable variation was found between cork oak stands of the different areas. Results of the principal component analysis performed on the cork quality data and environmental characters of the eight areas, showed that the first three components explain 72.2% of the variation. The major characters involved in this differentiation were cork quality characters such as dimensional recover, moisture and Mg content of the cork and also elevation of stands m a.s.l. for the first component. The second component appears to be determined by some climatic parameters (average annual temperature and average of the minimum temperatures of coldest month) and by the Fe and Zn in the cork. For the third component, major characters involved in differentiation are average annual rainfall, bark thickness and Ca content in the cork.

[1]  F. Maetzke,et al.  Influenza del clima sulla crescita del sughero in soprassuoli produttivi siciliani , 2009 .

[2]  V. Clark,et al.  Computer-aided multivariate analysis , 1991 .

[3]  S. Arulsekar,et al.  Isozyme Analysis Procedures for Stone Fruits, Almond, Grape, Walnut, Pistachio, and Fig , 1986, HortScience.

[4]  V. Sork,et al.  ASSOCIATION BETWEEN ENVIRONMENTAL AND GENETIC HETEROGENEITY IN FOREST TREE POPULATIONS , 2001 .

[5]  F. Yeh,et al.  RAPD variation within and among natural populations of trembling aspen (Populus tremuloides Michx.) from Alberta. , 1995, The Journal of heredity.

[6]  F. Rohlf,et al.  NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, version 2.1: Owner manual , 1992 .

[7]  L. Toumi,et al.  Allozyme variation in cork oak (Quercus suber L.): the role of phylogeography and genetic introgression by other Mediterranean oak species and human activities , 1998, Theoretical and Applied Genetics.

[8]  J. P. Martín,et al.  Fingerprinting and genetic variability in cork oak (Quercus suber L.) elite trees using ISSR and SSR markers , 2007, Annals of Forest Science.

[9]  H. Pereira,et al.  Caractérisation de la croissance et de la qualité du liège dans une région de production , 2000 .

[10]  I. Vogiatzakis,et al.  Mapping the distribution and extent of Quercus suber habitats in Sardinia : a literature review and a proposed methodology , 2003 .

[11]  R. Jorgensen,et al.  Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[12]  L. R. Dice Measures of the Amount of Ecologic Association Between Species , 1945 .

[13]  J. Francisco Duque-Carrillo,et al.  Cork quality classification system using a unified image processing and fuzzy-neural network methodology , 1997, IEEE Trans. Neural Networks.

[14]  D. Dettori,et al.  Performance di recenti imboschimenti con quercia da sughero in ex-coltivi , 2006 .

[15]  L. Excoffier,et al.  Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. , 1992, Genetics.

[16]  S. Dettori,et al.  Quantità e qualità delle produzioni sughericole regionali , 2008 .

[17]  A. Cravador,et al.  Genetic Diversity of Two Evergreen Oaks [Quercus suber (L.) and Quercus ilex subsp. rotundifolia (Lam.)] in Portugal using AFLP Markers , 2006 .

[18]  B. Schirone,et al.  Utility of ITS sequence data for phylogenetic reconstruction of Italian Quercus spp. , 2005, Molecular phylogenetics and evolution.

[19]  Helena Pereira,et al.  The Evaluation of the Quality of Cork Planks by Image Analysis , 1996 .

[20]  C. Vallejos Enzyme Activity Staining , 1983 .

[21]  K. Oyama,et al.  Genetic variation and differentiation of populations within the Quercus affinis Quercus laurina (Fagaceae) complex analyzed with RAPD markers , 2005 .

[22]  Tabor oak in Israel, genetic diversity within and between populations , 2004 .

[23]  Norman Louat,et al.  The evaluation of , 1974 .

[24]  R. Yang,et al.  POPGENE Version 1.32 Microsoft Windows-based freeware for populations genetic analysis. University of Alberta, Edmonton , 1999 .

[25]  J. Chemnitius,et al.  Identification of isoenzymes in cholinesterase preparations using kinetic data of organophosphate inhibition. , 1982, Analytical biochemistry.

[26]  R. Lewontin The Apportionment of Human Diversity , 1972 .

[27]  P. V. Arrigoni FITOCLIMATOLOGIA DELLA SARDEGNA , 1968 .

[28]  R. Dodd,et al.  Genetic differentiation of two California red oak species, Quercus parvula var. shreveii and Q. wislizeni , based on AFLP genetic markers , 2002 .

[29]  A. Newton,et al.  Genetic variation in Fitzroya cupressoides (alerce), a threatened South American conifer , 1999, Molecular ecology.

[30]  M. Zarek RAPD analysis of genetic structure in four natural populations of Taxus baccata from southern Poland , 2009 .

[31]  Donato Salvatore La Mela Veca,et al.  Differences of raw cork quality in productive cork oak woods in Sicily in relation to stand density , 2010 .

[32]  P. Corona,et al.  Site quality evaluation by classification tree: an application to cork quality in Sardinia , 2005, European Journal of Forest Research.

[33]  SSR markers for Quercus suber tree identification and embryo analysis. , 2001, The Journal of heredity.

[34]  K. Livak,et al.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. , 1990, Nucleic acids research.